LOCUS       sequence-318469-        9390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expressing iaaH to convert auxin precursors (IAM or NAM) to 
            auxin (IAA or NAA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Ma Y, Budde MW, Mayalu MN, Zhu J, Lu AC, Murray RM, Elowitz MB
  TITLE     Synthetic mammalian signaling circuits for robust cell population 
            control.
  JOURNAL   Cell. 2022 Mar 17;185(6):967-979.e12. doi: 
            10.1016/j.cell.2022.01.026. Epub 2022 Mar 1.
  PUBMED    35235768
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.01.026"; journalName: "Cell"; date: 
            "2022-03-17- 17"; volume: "185"; issue: "6"; pages: "967-979.e12"
COMMENT     Sequence Label: vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hy...
FEATURES             Location/Qualifiers
     source          1..9390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             306..326
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             330..350
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             354..374
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(390..392,393..1103)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(432..453)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(693..712)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1040..1061
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1104..1157
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(2582..2599)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2588..2795
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2828..2848)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2833..3162
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3013..3148
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3075..3094
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3211..4236
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3211..4236
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(4296..4316)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    4358..4479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4395..4414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4449..4468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5242..5276
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5767..5783)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5767..5783)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5780..5802)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5791..5807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5815..5821,5822..5839,5840..5845))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5860..5881
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5998..6015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6169..6757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6249..6268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6928..7719,7720..7788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7551..7570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7789..7893)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7919..8374)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8056..8077)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8268..8287
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8501..8523
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8515..8532
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8516..8532
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8662..8724
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ggggctagca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacaccgg
       61 ttgagttgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgtgtc cgccgtctag
      121 gtaagtttaa agctcaggtt gagaccgggc ctttgtccgg tgctcccttg gagcctacct
      181 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
      241 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac ctgggcctac ggatccgcca
      301 ccatgcccaa aaaaaagcga aaagtgggtc ctaagaagaa acggaaggtt ggccccaaaa
      361 agaaacggaa ggtcggtagt ggtggcagtg tgagcaaggg cgaggagctg ttcaccgggg
      421 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      481 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      541 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct
      601 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      661 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      721 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      781 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      841 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      901 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      961 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     1021 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1081 tcggcatgga cgagctgtac aaggagggca gggggagcct cctaacttgc ggagatgtcg
     1141 aggagaatcc tggcccaggt ggagtagcca tcacgtcact ggcccaatct ttggagcatc
     1201 ttaaacgaaa agactattct tgtcttgaat tggttgagac acttattgcc agatgcgagg
     1261 ccgcgaaaag tcttaatgct cttctcgcaa cggattggga cgggttgagg agatccgcta
     1321 agaagataga ccggcatgga aatgctgggg ttggactctg tgggataccg ctgtgtttca
     1381 aggcaaacat cgccacgggt gtgttcccca cgagtgcggc cacgccagcc ttgattaacc
     1441 accttcctaa aatcccatcc cgcgtagcag aaaggctgtt ttccgcaggc gctcttccag
     1501 gggcttccgg aaatatgcac gagcttagtt ttggcattac tagcaacaac tatgctactg
     1561 gggccgtgcg caacccatgg aacccagacc tcattccggg tgggagttct ggcggtgtcg
     1621 ccgccgcggt agcgtcacgc ttgatgctgg ggggaatcgg aaccgacaca ggggccagcg
     1681 taaggttgcc agcagccctg tgtggcgtcg taggcttccg acctacactc ggacgctacc
     1741 cgggcgaccg cattatccct gttagtccca ctcgcgatac tcctggtatt atcgctcaat
     1801 gtgtggctga cgtcgtcatc ctggatcgga tcataagtgg tacaccagaa cgaatcccac
     1861 cggttccgct taaaggcctc cggattgggc tgcccacgac ctatttttat gatgacctcg
     1921 atgccgatgt cgccttggcg gcggagacta cgattagact tcttgcgaac aaaggggtca
     1981 cgtttgttga ggctaacata ccgcacttgg acgagttgaa taaaggggcg tcttttcccg
     2041 tggcactgta tgaatttccg cacgctctta aacagtacct ggacgatttc gttaaaacgg
     2101 ttagcttttc agatgtaata aagggcatcc gatcaccaga tgtggctaat atagctaatg
     2161 cccaaattga cggtcatcaa atttccaaag ccgagtatga actcgcccgc cacagctttc
     2221 ggccaagact tcaggcgaca tatcgaaatt actttaagct taatcgcttg gatgcgatac
     2281 ttttccccac cgcccctctg gtagccagac cgattggtca agactcttca gttatacaca
     2341 atggaactat gcttgataca tttaagatat atgtacggaa cgtagaccca agctcaaatg
     2401 cagggctccc gggcttgtca attccagttt gccttacgcc ggaccggctt ccagtgggga
     2461 tggaaataga tggacttgcg gactctgacc aacgccttct cgctataggc ggtgccctgg
     2521 aagaagcgat tgggtttaga tatttcgctg gacttccgaa cacctagcga atttaaatcg
     2581 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     2641 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     2701 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     2761 gaggattggg aagacaatag caggcatgct ggggacgcgg ccgcaattta acgcgaatta
     2821 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2881 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     2941 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3001 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3061 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     3121 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     3181 atatccattt tcggatctga tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct
     3241 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     3301 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     3361 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     3421 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     3481 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     3541 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     3601 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     3661 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     3721 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     3781 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     3841 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     3901 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     3961 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     4021 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     4081 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     4141 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     4201 cgacgcccca gcactcgtcc gagggcaaag gaataggtcg acaatcaacc tctggattac
     4261 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4321 tacgctgctt taatgccttt gtatcatgcg ttaactaaac ttgtttattg cagcttataa
     4381 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4441 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa
     4501 atgatgtcaa ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc
     4561 ctgtttaata tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc
     4621 aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag
     4681 ccaggtttag ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt
     4741 ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag
     4801 cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg
     4861 cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg
     4921 gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca
     4981 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
     5041 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
     5101 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
     5161 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
     5221 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
     5281 tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct
     5341 ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg
     5401 catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt
     5461 ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc
     5521 aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc
     5581 cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc
     5641 agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct
     5701 ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta
     5761 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5821 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5881 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5941 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6001 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6061 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6601 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6901 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7261 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7321 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7381 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7441 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7501 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7561 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7621 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7681 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7741 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7801 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7861 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     7921 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     7981 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     8041 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8101 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8161 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8221 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8281 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8341 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     8401 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     8461 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     8521 acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga ggacgggcag
     8581 actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg
     8641 cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta cgttaaagat
     8701 aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt ttatttatta
     8761 atttgaatag atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca
     8821 aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa tttcttctat
     8881 aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg atgtaattac
     8941 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
     9001 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
     9061 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
     9121 tacggggaaa aggcctggat ctgccattgc tccagtgccc ttcagtgggc agagagcaca
     9181 tcacccacag tccccaagaa gttgggggga ggggtgggca attgaactgg tgcctagaga
     9241 aggtggcgca gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     9301 ggtgggggag aaccatatat aagtgcagta gtccccctga acgttctttt ttgcaacggg
     9361 tttgccgcca gaacacagct gaagctttga
//
