LOCUS       sequence-318474-       12632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expressing the full paradoxical circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12632)
  AUTHORS   Ma Y, Budde MW, Mayalu MN, Zhu J, Lu AC, Murray RM, Elowitz MB
  TITLE     Synthetic mammalian signaling circuits for robust cell population 
            control.
  JOURNAL   Cell. 2022 Mar 17;185(6):967-979.e12. doi: 
            10.1016/j.cell.2022.01.026. Epub 2022 Mar 1.
  PUBMED    35235768
REFERENCE   2  (bases 1 to 12632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.01.026"; journalName: "Cell"; date: 
            "2022-03-17- 17"; volume: "185"; issue: "6"; pages: "967-979.e12"
COMMENT     Sequence Label: 
            vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-B
            lastR
FEATURES             Location/Qualifiers
     source          1..12632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        158..1336
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          389..1327
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1284..1304
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1359..3083
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             3084..3137
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4545..4598
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5334..5537
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             5556..5876
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(6228..6239)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6753..6779
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6786..6839
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6840..7544
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6982..7000)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7250..7269
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7566..7769
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             7782..7805
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7821..8219
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8221..8342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8258..8277)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8312..8331
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8865..8899
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9390..9406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9390..9406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9403..9425)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9414..9430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9438..9444,9445..9462,9463..9468))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9483..9504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9621..9638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9792..10380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9872..9891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10551..11342,11343..11411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11174..11193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11412..11516)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11542..11997)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11679..11700)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11891..11910
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12124..12146
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12138..12155
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12139..12155
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   12285..12347
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
       61 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
      121 aaggcctcca cggccagcac ctgcgtgacg tacgtgaggc tccggtgccc gtcagtgggc
      181 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      241 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      301 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      361 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      421 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      481 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      541 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      601 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      661 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      721 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      781 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      841 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      901 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      961 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1021 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1081 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1141 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1201 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1261 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1321 atttcaggtg tcgtgaacct tcacgcaggt gggccaccat gacatacttt cctgaagagg
     1381 tcgtcgaaca catttttagc ttcctgcctg cacagagaga tagaaacaca gtgagcctgg
     1441 tctgcaaagt gtggtacgag atcgaacgcc tgagccggag aggagtgttc gtcggcaact
     1501 gctatgctgt gagagcaggc agggtcgccg ctaggtttcc aaatgtgcgc gcactgaccg
     1561 tcaaggggaa accccacttc gccgacttta acctggtgcc ccctgattgg ggaggatacg
     1621 ccggcccttg gatcgaggca gccgctcgcg gctgtcatgg actggaggaa ctgcgcatga
     1681 agcgaatggt ggtctctgac gaaagtctgg agctgctggc tcggagcttc cctaggtttc
     1741 gcgcactggt gctgatttct tgcgaaggct tcagcaccga tggactggca gccgtggcct
     1801 cccactgtaa gctgctgcgg gagctggacc tccaggagaa tgaagtggag gatagaggcc
     1861 ccagatggct gtcttgcttc ccagactcat gtaccagcct ggtgtccctg aactttgcct
     1921 gcatcaaagg cgaagtgaat gctgggtccc tggagcggct ggtctcaaga agccccaacc
     1981 tgaggtctct gcggctgaac cggagcgtga gcgtggacac tctggctaag attctgctga
     2041 gaacccctaa cctggaggat ctgggaaccg gcaatctgac agacgatttc cagacagaat
     2101 cctactttaa actgacttct gccctggaga agtgtaaaat gctgaggagt ctgtcaggat
     2161 tctgggatgc ttcacccgtg tgcctgagct ttatctaccc tctgtgtgca cagctgacag
     2221 gcctgaacct gagctatgca ccaaccctgg acgccagtga tctgacaaag atgatctcac
     2281 gctgcgtgaa actccagcga ctgtgggtgc tggactgtat ttccgataag gggctccagg
     2341 tggtcgccag ctcctgcaag gacctccagg agctgagagt gttcccatct gatttttacg
     2401 tggccggata tagtgctgtc actgaggaag gcctggtggc agtctcactg ggatgcccaa
     2461 agctgaacag cctgctgtat ttctgtcatc agatgactaa tgctgcactg gtgaccgtcg
     2521 ccaagaactg ccctaatttc acccgatttc ggctgtgtat tctggaacca ggcaaacccg
     2581 acgtggtcac atcccagcca ctggatgaag ggtttggagc tatcgtgaga gagtgcaagg
     2641 gactccagag gctgagcatt tccggcctgc tgacagacaa agtgttcatg tacatcggca
     2701 agtatgctaa gcagctggag atgctgagca ttgcatttgc cggagactcc gataagggca
     2761 tgatgcacgt gatgaacggg tgtaagaatc tgcgaaaact ggaaatccgg gacagccctt
     2821 tcggggatgc cgctctgctg ggaaactttg ccagatacga gacaatgagg agcctgtgga
     2881 tgtctagttg caatgtgact ctgaagggct gtcaggtcct ggctagtaaa atgcctatgc
     2941 tgaacgtgga agtcattaat gagcgggacg ggtctaacga aatggaggaa aatcatggcg
     3001 acctgccaaa ggtggagaaa ctgtatgtgt atcggaccac cgcaggggca agagatgatg
     3061 ctcccaactt tgtgaagatt ctggagggca gggggagcct cctaacttgc ggagacgtcg
     3121 aggagaatcc tggcccaggt ggagtagcca tcacgtcact ggcccaatct ttggagcatc
     3181 ttaaacgaaa agactattct tgtcttgaat tggttgagac acttattgcc agatgcgagg
     3241 ccgcgaaaag tcttaatgct cttctcgcaa cggattggga cgggttgagg agatccgcta
     3301 agaagataga ccggcatgga aatgctgggg ttggactctg tgggataccg ctgtgtttca
     3361 aggcaaacat cgccacgggt gtgttcccca cgagtgcggc cacgccagcc ttgattaacc
     3421 accttcctaa aatcccatcc cgcgtagcag aaaggctgtt ttccgcaggc gctcttccag
     3481 gggcttccgg aaatatgcac gagcttagtt ttggcattac tagcaacaac tatgctactg
     3541 gggccgtgcg caacccatgg aacccagacc tcattccggg tgggagttct ggcggtgtcg
     3601 ccgccgcggt agcgtcacgc ttgatgctgg ggggaatcgg aaccgacaca ggggccagcg
     3661 taaggttgcc agcagccctg tgtggcgtcg taggcttccg acctacactc ggacgctacc
     3721 cgggcgaccg cattatccct gttagtccca ctcgcgatac tcctggtatt atcgctcaat
     3781 gtgtggctga cgtcgtcatc ctggatcgga tcataagtgg tacaccagaa cgaatcccac
     3841 cggttccgct taaaggcctc cggattgggc tgcccacgac ctatttttat gatgacctcg
     3901 atgccgatgt cgccttggcg gcggagacta cgattagact tcttgcgaac aaaggggtca
     3961 cgtttgttga ggctaacata ccgcacttgg acgagttgaa taaaggggcg tcttttcccg
     4021 tggcactgta tgaatttccg cacgctctta aacagtacct ggacgatttc gttaaaacgg
     4081 ttagcttttc agatgtaata aagggcatcc gatcaccaga tgtggctaat atagctaatg
     4141 cccaaattga cggtcatcaa atttccaaag ccgagtatga actcgcccgc cacagctttc
     4201 ggccaagact tcaggcgaca tatcgaaatt actttaagct taatcgcttg gatgcgatac
     4261 ttttccccac cgcccctctg gtagccagac cgattggtca agactcttca gttatacaca
     4321 atggaactat gcttgataca tttaagatat atgtacggaa cgtagaccca agctcaaatg
     4381 cagggctccc gggcttgtca attccagttt gccttacgcc ggaccggctt ccagtgggga
     4441 tggaaataga tggacttgcg gactctgacc aacgccttct cgctataggc ggtgccctgg
     4501 aagaagcgat tgggtttaga tatttcgctg gacttccgaa caccgaggga cgcggatcac
     4561 tgcttacttg cggggatgtg gaggagaatc ccggtcccat ggctagcaaa ggtgaggagc
     4621 tttttactgg tgtcgttccc atcctcgtag aactggacgg agatgtcaac ggccataagt
     4681 tttcagtttc cggtgaaggc gagggtgacg caacatacgg taagctgaca ctgaaattca
     4741 tttgtacaac cgggaaactc cccgtcccct ggcccaccct ggttaccacc ttcggttatg
     4801 gagtacagtg ctttgctaga tatcctgatc acatgaagca acatgatttc tttaaaagcg
     4861 ccatgccaga gggctatgtg caagagagga ctatattctt taaagacgac ggaaactata
     4921 aaactagagc agaggtaaag tttgaggggg atacacttgt aaatcggata gaactgaagg
     4981 ggatcgactt taaagaggat ggaaatattc tcggtcacaa actggaatat aactacaact
     5041 cccacaatgt ctatatcatg gctgacaagc agaaaaacgg gattaaagtg aacttcaaga
     5101 tccgccacaa catcgaagac ggcagtgtgc aacttgcaga ccactaccaa caaaacacac
     5161 ctattggcga cggtccagtt ttgctgcccg acaatcatta tctttctact caaagcaaac
     5221 tttcaaaaga ccccaatgaa aagcgggacc acatggttct tctggaattt gtaaccgctg
     5281 ccgggataac acatggaatg gatgagttgt acaagggatc cggtgcaggc gccaaggaga
     5341 agagtgcttg tcctaaagat ccagccaaac ctccggccaa ggcacaagtt gtgggatggc
     5401 caccggtgag atcataccgg aagaacgtga tggtttcctg ccaaaaatca agcggtggcc
     5461 cggaggcggc ggcgttcgtg aaggtatcaa tggacggagc accgtacttg aggaaaatcg
     5521 atttgaggat gtataaaggt ggatccggac tcgagggagt gcaggtggag actatctccc
     5581 caggagacgg gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga
     5641 tgcttgaaga tggaaagaaa gttgattcct cccgggacag aaacaagccc tttaagttta
     5701 tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg
     5761 gtcagagagc caaactgact atatctccag attatgccta tggtgccact gggcacccag
     5821 gcatcatccc accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaatctg
     5881 gcggtggatc cggagtcgac ggatttggtg atgtcggtgc tcttgagagt ttgaggggaa
     5941 atgcagattt ggcttacatc ctgagcatgg agccctgtgg ccactgcctc attatcaaca
     6001 atgtgaactt ctgccgtgag tccgggctcc gcacccgcac tggctccaac atcgactgtg
     6061 agaagttgcg gcgtcgcttc tcctcgctgc atttcatggt ggaggtgaag ggcgacctga
     6121 ctgccaagaa aatggtgctg gctttgctgg agctggcgcg gcaggaccac ggtgctctgg
     6181 actgctgcgt ggtggtcatt ctctctcacg gctgtcaggc cagccacctg cagttcccag
     6241 gggctgtcta cggcacagat ggatgccctg tgtcggtcga gaagattgtg aacatcttca
     6301 atgggaccag ctgccccagc ctgggaggga agcccaagct ctttttcatc caggcctgtg
     6361 gtggggagca gaaagaccat gggtttgagg tggcctccac ttcccctgaa gacgagtccc
     6421 ctggcagtaa ccccgagcca gatgccaccc cgttccagga aggtttgagg accttcgacc
     6481 agctggacgc catatctagt ttgcccacac ccagtgacat ctttgtgtcc tactctactt
     6541 tcccaggttt tgtttcctgg agggacccca agagtggctc ctggtacgtt gagaccctgg
     6601 acgacatctt tgagcagtgg gctcactctg aagacctgca gtccctcctg cttagggtcg
     6661 ctaatgctgt ttcggtgaaa gggatttata aacagatgcc tggttgcttt aatttcctcc
     6721 ggaaaaaact tttctttaaa acatcagtcg actatccgta cgacgtacca gactacgcac
     6781 tcgacgaggg aaggggctcc ttgcttactt gcggagacgt cgaggagaat cctggcccag
     6841 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     6901 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     6961 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     7021 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     7081 ccgacattcc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     7141 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     7201 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     7261 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     7321 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     7381 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     7441 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     7501 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtctgga tccggtgcag
     7561 gcgccaagga gaagagtgct tgtcctaaag atccagccaa acctccggcc aaggcacaag
     7621 ttgtgggatg gccaccggtg agatcatacc ggaagaacgt gatggtttcc tgccaaaaat
     7681 caagcggtgg cccggaggcg gcggcgttcg tgaaggtatc aatggacgga gcaccgtact
     7741 tgaggaaaat cgatttgagg atgtataaag gaggatccgg tgactataag gacgatgacg
     7801 acaagggtgg tggagggtcc atggccaagc ctttgtctca agaagaatcc accctcattg
     7861 aaagagcaac ggctacaatc aacagcatcc ccatctctga agattacagc gtcgccagcg
     7921 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     7981 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     8041 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     8101 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     8161 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctgac
     8221 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8281 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8341 taggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc
     8401 agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc
     8461 cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg
     8521 ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg
     8581 ggctgtccct gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga
     8641 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa
     8701 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat
     8761 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga
     8821 cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac
     8881 gcagactatc tttctagggt taatctagct gcatcaggat catatcgtcg ggtctttttt
     8941 ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc
     9001 cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat
     9061 tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc
     9121 ctttaacggt gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg
     9181 gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag
     9241 ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt
     9301 cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt
     9361 acccggcggg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9421 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9481 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    10021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    10081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    10141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    10201 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    10261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    10321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    10381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    10441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10681 atgataccgc gagatccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10861 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    11041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    11101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    11161 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    11221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    11281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    11341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    11401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    11461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11521 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
    11581 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
    11641 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
    11701 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
    11761 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
    11821 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
    11881 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
    11941 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
    12001 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    12061 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    12121 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
    12181 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
    12241 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
    12301 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
    12361 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
    12421 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
    12481 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
    12541 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
    12601 gggctccgct ccggtccggc gctccccccg ca
//
