LOCUS       sequence-318478-        4288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mobius Assembly cloning and expression vector for plants with 
            high-copy number origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: pMAP L1A
FEATURES             Location/Qualifiers
     source          1..4288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      54..642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     543..562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2164..2188
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    2215..2239
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2288..2322
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2331..2342
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3031..3102
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3118..3145
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3375..4190)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4182..4201
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 caagatcgga ttggcggtta tgcggttccc gtagaaagga tcaaaggatc ttcttgagat
       61 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      181 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      421 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgccgatcc gccgccagtt
      781 caggccgtcc ggccccggat tctgaccata atttcatcga aaaaaggggc gcagcccttc
      841 ttgttctaat agttctataa gttcaggcga aaatcgtgca gcaattacaa aaggttgcgc
      901 gtctataagt ggggaatcca gacgcaaaag tggggaatcc agccgcaaaa gtggggaatc
      961 cagccgaaat cgcggattga cgagtgggat ttcccgccca ataaatccac catgggaaag
     1021 acactcgacg ttgcccgcga ccgggccttt gaccagaccg cgaccgtgct gcccgccgaa
     1081 atggcgcggg gggtctatat gcgcaacgcc cccagcctcg cggccctgaa gctgatgcat
     1141 ctgatgatcg ccacggcggg cgggcgcatg gccgatgacg tgcgccacga aatgcggctg
     1201 gccgacatcc gcaagatcga cggtatggat aaccacaccc gggccagcct gaccccgctc
     1261 tttgcggaac tggcggcggc ggtgctgacc cacgacgacc cggaaaagcg ggtcgtgacc
     1321 atcggtggcc tgctggacga agcccggatc gattaccggc acgaggtcag cggcgatctt
     1381 ctggtgtcgt ggaccttccg cagcatgttc cgccgcatgg cggcggaatc gaaccattgg
     1441 gcgattctcg accggcagac cgtgttccac ctcggcagca agtattccgt gctgttgttc
     1501 cagcacatcg ccagcttcaa ggaatacgac cacattaccg gcaagacctt taccgtgccg
     1561 gagttgcggg ctgtgtttgg tatccccgag ggcaaaatca agcgtttcgc agacctcaac
     1621 agagacgtgc tgacgcccgc cattgccgaa atcaaccagc tttcccgcct gactctgacc
     1681 gccacgccga acaagatcgg gcgcaccgtg gccagcgtga cgattgcttg ggaagaaaag
     1741 cccctcgaag gcaagcgctc gaccaaggcc gaactggacc gcccgaaggt gggccggaag
     1801 gcccggcgcg acggcaccgc cgagacggtg gcacgggcct tcccggcatc gggcgggatc
     1861 gagttcgacc agcattggcg cgacctcaag cgggcggcgg gctgcaacat ggacaacacc
     1921 atgatcgccg acaaattccg ggcatggtgc gccgggaagg gcctcgctct cgatgcccgg
     1981 aacatcgaac aggcgttcag cagcttctgc accaaggtgg gccgggtctg accatcatca
     2041 gttcggtggt cttccgacga acaataaggc cgcaaatcgc ggcctttttt attgataaca
     2101 aaaccggctc agttctgcgt agaaaccaac atgcaagctc caccgggtgc aaagcggcag
     2161 cggcggcagg atatattcaa ttgtaaatgg cttcatgtcc gggaaatcta ctggtggcag
     2221 gatatattgt ggtgtaaaca atggagaaaa agagaattgg aattccacct gcatatggag
     2281 agagaccttt acggctagct cagtcctagg tatagtgcta gctactagag aaagaggaga
     2341 aatactaaat gtcgcactca aaacaagcac tggcagatac gatgaaaatg acgtggctga
     2401 tggaaggttc ggtcaacggt cacgccttta cgattgaagg cgaaggtacc ggcaaaccgt
     2461 atgaaggtaa acagagtggc acctttcgtg ttacgaaagg cggtccgctg ccgtttgcgt
     2521 ttgatattgt cgctccgacc ctgaaatacg gtttcaaatg cttcatgaaa tacccggcgg
     2581 atattccgga ctactttaaa ctggccttcc cggaaggcct gacgtacgat cgtaaaattg
     2641 cgtttgaaga cggcggttgt gcgaccgcca cggtcgaaat gagcctgaag ggtaacaccc
     2701 tggtgcataa aacgaacttt cagggcggta atttcccgat tgatggtccg gtgatgcaaa
     2761 aacgcaccct gggctgggaa ccgacctctg aaaaaatgac gccgtgcgat ggtattatca
     2821 aaggcgacac catcatgtac ctgatggttg aaggcggtaa aacgctgaaa tgtcgttatg
     2881 aaaacaatta ccgcgccaac aaaccagtgc tgatgccgcc gagccacttt gtggatctgc
     2941 gtctgacccg cacgaatctg gataaagaag gtctggcgtt caaactggaa gaatatgctg
     3001 ttgcccgtgt gctggaagtg taataagctt caaataaaac gaaaggctca gtcgaaagac
     3061 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     3121 tcaccttcgg gtgggccttt ctgcgggtct cacgctcaga atatgcaggt gctgcaggaa
     3181 ttgttgattt tgtgatgact gatggcagga tatatgcggt tgtaattcat ttttattgtc
     3241 taaatttctg tatttgtttg tttgttcggt tgtaaatttt tttggaagaa caagaaaaga
     3301 aaaaacaccc gttagggtgt ttttagttag tgtggcgcgc cgacttgcga catgcggtcc
     3361 tttgcaatca actattagaa aaattcatcc agcatcagat gaaattgcag tttgttcata
     3421 tccggattat caatgccata tttctgaaac agacgttttt gcaggctcgg gctaaattcg
     3481 cccaggcagt tccacagaat ggccagatcc tgataacgat ccgcaatgcc cacacggccc
     3541 acatcaatgc agccaatcag tttgccttca tcgaaaatca ggttatccag gctaaaatcg
     3601 ccgtgggtca ccacgctatc cgggctaaac ggcagcagtt tatgcatttc tttccacacc
     3661 tgttccaccg gccagccgtt acgttcatca tcaaaatcgc tcgcatccac caggccgttg
     3721 ttcatacggc tctgcgcctg ggccagacga aacacacgat cgctgttaaa cgggcagttg
     3781 cacaccggaa tgctatgcag acgacgcaga aacacggcca gcgcatccac aatgttttcg
     3841 ccgctatccg gatattcttc cagcacctga aacgcggttt tgcccggaat cgcggtggtc
     3901 agcagccacg catcatccgg ggtgcgaata aaatgtttaa tggtcggcag cggcataaat
     3961 tcggtcagcc agttcagacg caccatttca tcggtcacat cgttcgccac gctgcctttg
     4021 ccatgtttca gaaacagttc cggcgcatcc ggtttgccat acagacgata aatggtcgcg
     4081 ccgctctgac ccacgttatc acgcgcccat ttatagccat acagatccgc atccatgttg
     4141 ctgttcagac gcggacggct acagctcgtt tcacgctgaa tatggctcat aacacccctt
     4201 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     4261 gcaatgtaac atcagagatt ttgagaca
//
