LOCUS       sequence-318480-        3740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLX series: RK2-based T-DNA binary vector for plant transformation 
            (alpha1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins.
  JOURNAL   ACS Synth Biol. 2017 Oct 20;6(10):1962-1968. doi: 
            10.1021/acssynbio.6b00354. Epub 2017 Jun 28.
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00354"; journalName: "ACS Synth Biol"; date: 
            "2017-10-20- 20"; volume: "6"; issue: "10"; pages: "1962-1968"
COMMENT     Sequence Label: pLX-Z4 (GB3375)
FEATURES             Location/Qualifiers
     source          1..3740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(56..62,63..80,81..86))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(381..914)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1103..1108,1109..1125,1126..1131))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             complement(1206..2354)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      2747..3278
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    3410..3434
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tcattgttgt actccggtta ggacggattg ggaactggct aactcaaaat ccacacatta
       61 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg tctctcgctg
      121 tcaggagacg ggacaaggat gcgcctgcag gttgttgatg atgtgatgac tgatggcagg
      181 atatatgtgg ttgtaattca tttctaccgt gtaatttact gtattttttt gtttgttcgt
      241 tcgtttgtaa aaatattttt tggaagcaaa aaattagcgc aagaagacaa aaatcacctt
      301 gcgctaatgc tctgttacag gcgcgccaat ttacccaaca actccgcggc cgggaagccg
      361 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
      421 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
      481 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
      541 gcggtggcaa tgccctgcct ccggtgctct ccggagactg cgagatcata gatatagatc
      601 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
      661 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
      721 tcggagtccg gctgatgttg ggagtaggtg gctacatcgc cgaactcacg accgaaaaga
      781 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
      841 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
      901 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
      961 ttgctgcttg gatgcccgag gcataggctg tacaaaaaaa cagtcataac aagccatgaa
     1021 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1081 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca agatcggatt
     1141 ggcggttatg cggttgcgat gcaggtggct gctgaacccc cagccggaac tgaccccaca
     1201 aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg
     1261 ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg
     1321 gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc
     1381 gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat
     1441 atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc
     1501 tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc
     1561 gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac
     1621 aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa
     1681 agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac
     1741 acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc
     1801 ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc
     1861 ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg
     1921 tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg
     1981 tgtttcagca acgcggcctg cttggcttcg ctgacctgtt ttgccaggtc ctcgccggcg
     2041 gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa
     2101 cctgccgcct cctgttctag ccgacgcgaa cgctccacgg cggccgatgg cgcgggcagg
     2161 gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggactatc
     2221 gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt
     2281 tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca
     2341 aacgtccgat tcattcaccc tccttgcggg attgccccgg aattaattcc ccggatcgat
     2401 ccgtcgatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     2461 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg
     2521 ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct
     2581 ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     2641 gtcagcaccg tttctgcgga ctggctttct acgtggctgc catttttggg gtgaggccgt
     2701 tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg
     2761 gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc
     2821 tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg
     2881 tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc
     2941 tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat
     3001 cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc
     3061 cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt
     3121 tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac
     3181 gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg
     3241 gccaagtttt ccgcgaggta tccacaacgc cggcggccct acatggctct gctgtagtga
     3301 gtgggttgcg ctccggcagc ggtcctgatc ccccgcagaa aaaaaggatc tcaagaagat
     3361 cctttgatct tttctacagg cctgctggta atcgcaggcc tttttatttt ggcaggatat
     3421 attgtggtgt aaacacttac cgcacctctg cagcagcggc aggatatatg gcagtgtaaa
     3481 ctccattttc gaacgcgtta attaagtagc ctggtcgaaa ccgtctcagg agagagacca
     3541 aaagcaaaaa cccgccgaag cgggttacta gcgccattcg ccattcagag agcggagctg
     3601 ctgcgacgga cgatcggtac gcgcctcttc gctattacgc caactggcga aaggtggatg
     3661 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtagtac
     3721 cacggcaagg ctatctgtaa
//
