LOCUS       sequence-318483-        3287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLX series: pBBR1-based T-DNA binary vector for plant transformation
            (alpha2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins.
  JOURNAL   ACS Synth Biol. 2017 Oct 20;6(10):1962-1968. doi: 
            10.1021/acssynbio.6b00354. Epub 2017 Jun 28.
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00354"; journalName: "ACS Synth Biol"; date: 
            "2017-10-20- 20"; volume: "6"; issue: "10"; pages: "1962-1968"
COMMENT     Sequence Label: pLX-B2alpha2 (GB1696)
FEATURES             Location/Qualifiers
     source          1..3287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    737..761
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    788..812
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        complement(join(1135..1141,1142..1159,1160..1165))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(1429..2244)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2236..2255
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(2370..3032)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      join(3033..3287,1..517)
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 cccctgcggg gttcccccac gccttcggcg cggctccccc tccggcaaaa agtggcccct
       61 ccggggcttg ttgatcgact gcgcggcctt cggccttgcc caaggtggcg ctgccccctt
      121 ggaacccccg cactcgccgc cgtgaggctc ggggggcagg cgggcgggct tcgcccttcg
      181 actgccccca ctcgcatagg cttgggtcgt tccaggcgcg tcaaggccaa gccgctgcgc
      241 ggtcgctgcg cgagccttga cccgccttcc acttggtgtc caaccggcaa gcgaagcgcg
      301 caggccgcag gccggaggct tttccccaga gaaaattaaa aaaattgatg gggcaaggcc
      361 gcaggccgcg cagttggagc cggtgggtat gtggtcgaag gctgggtagc cggtgggcaa
      421 tccctgtggt caagctcgtg ggcaggcgca gcctgtccat cagcttgtcc agcagggttg
      481 tccacgggcc gagcgaagcg agccagccgg tggccgctcg cggccatcgt ccacatatcc
      541 acgggctggc aagggagcgc agcgaccgcg cagggcgaag cccggagagc aagcccgtag
      601 ggggccatca tcagttcggt ggtcttccga cgaacaataa ggccgcaaat cgcggccttt
      661 tttattgata acaaaaccgg ctcagttctg cgtagaaacc aacatgcaag ctccaccggg
      721 tgcaaagcgg cagcggcggc aggatatatt caattgtaaa tggcttcatg tccgggaaat
      781 ctactggtgg caggatatat tgtggtgtaa acaatggaga aaaagattaa ttaagtacta
      841 gtagcctggt cgaaaccgtc tcagtcagga gagagaccaa aagcaaaaac ccgccgaagc
      901 gggttactag cgccattcgc cattcagaga gcggagctgc tgcgacggac gatcggtacg
      961 cgcctcttcg ctattacgcc aactggcgaa aggtggatgt gctgcaaggc gattaagttg
     1021 ggtaacgcca gggttttccc agtcacgacg ttgtagtacc acggcaaggc tatctgtaat
     1081 cattgttgta ctccggttag gacggattgg gaactggcta actcaaaatc cacacattat
     1141 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt ctctcgctgg
     1201 agacgggaca aggatgcgag ctagcctgca ggaattgttg attttgtgat gactgatggc
     1261 aggatatatg cggttgtaat tcatttttat tgtctaaatt tctgtatttg tttgtttgtt
     1321 cggttgtaaa tttttttgga agaacaagaa aagaaaaaac acccgttagg gtgtttttag
     1381 ttagtgtggc gcgccgactt gcgacatgcg gtcctttgca atcaactatt agaaaaattc
     1441 atccagcatc agatgaaatt gcagtttgtt catatccgga ttatcaatgc catatttctg
     1501 aaacagacgt ttttgcaggc tcgggctaaa ttcgcccagg cagttccaca gaatggccag
     1561 atcctgataa cgatccgcaa tgcccacacg gcccacatca atgcagccaa tcagtttgcc
     1621 ttcatcgaaa atcaggttat ccaggctaaa atcgccgtgg gtcaccacgc tatccgggct
     1681 aaacggcagc agtttatgca tttctttcca cacctgttcc accggccagc cgttacgttc
     1741 atcatcaaaa tcgctcgcat ccaccaggcc gttgttcata cggctctgcg cctgggccag
     1801 acgaaacaca cgatcgctgt taaacgggca gttgcacacc ggaatgctat gcagacgacg
     1861 cagaaacacg gccagcgcat ccacaatgtt ttcgccgcta tccggatatt cttccagcac
     1921 ctgaaacgcg gttttgcccg gaatcgcggt ggtcagcagc cacgcatcat ccggggtgcg
     1981 aataaaatgt ttaatggtcg gcagcggcat aaattcggtc agccagttca gacgcaccat
     2041 ttcatcggtc acatcgttcg ccacgctgcc tttgccatgt ttcagaaaca gttccggcgc
     2101 atccggtttg ccatacagac gataaatggt cgcgccgctc tgacccacgt tatcacgcgc
     2161 ccatttatag ccatacagat ccgcatccat gttgctgttc agacgcggac ggctacagct
     2221 cgtttcacgc tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     2281 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     2341 cacaagatcg gattggcggt tatgcggttc taccggcgcg gcagcgttac ccgtgtcggc
     2401 ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc
     2461 tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc
     2521 ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc
     2581 ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg
     2641 tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg
     2701 ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc
     2761 acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa
     2821 gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc
     2881 atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg
     2941 gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct
     3001 tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta ggggtgccgc
     3061 acggttgcgg caccatgcgc aatcagctgc aacttttcgg cagcgcgaca acaattatgc
     3121 gttgcgtaaa agtggcagtc aattacagat tttctttaac ctacgcaatg agctattgcg
     3181 gggggtgccg caatgagctg ttgcgtaccc ccctttttta agttgttgat ttttaagtct
     3241 ttcgcatttc gccctatatc tagttctttg gtgcccaaag aagggca
//
