LOCUS       sequence-318484-        3349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLX series: pBBR1-based T-DNA binary vector for plant transformation
            (omega2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins.
  JOURNAL   ACS Synth Biol. 2017 Oct 20;6(10):1962-1968. doi: 
            10.1021/acssynbio.6b00354. Epub 2017 Jun 28.
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00354"; journalName: "ACS Synth Biol"; date: 
            "2017-10-20- 20"; volume: "6"; issue: "10"; pages: "1962-1968"
COMMENT     Sequence Label: pLX-B3omega2 (GB1695)
FEATURES             Location/Qualifiers
     source          1..3349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    762..786
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    813..837
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        complement(join(1160..1166,1167..1184,1185..1190))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(1444..2235)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(2457..3119)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      join(3120..3349,1..542)
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 gttctttggt gcccaaagaa gggcacccct gcggggttcc cccacgcctt cggcgcggct
       61 ccccctccgg caaaaagtgg cccctccggg gcttgttgat cgactgcgcg gccttcggcc
      121 ttgcccaagg tggcgctgcc cccttggaac ccccgcactc gccgccgtga ggctcggggg
      181 gcaggcgggc gggcttcgcc cttcgactgc ccccactcgc ataggcttgg gtcgttccag
      241 gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg
      301 gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa
      361 ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt
      421 cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg
      481 tccatcagct tgtccagcag ggttgtccac gggccgagcg aagcgagcca gccggtggcc
      541 gctcgcggcc atcgtccaca tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg
      601 cgaagcccgg agagcaagcc cgtagggggc catcatcagt tcggtggtct tccgacgaac
      661 aataaggccg caaatcgcgg ccttttttat tgataacaaa accggctcag ttctgcgtag
      721 aaaccaacat gcaagctcca ccgggtgcaa agcggcagcg gcggcaggat atattcaatt
      781 gtaaatggct tcatgtccgg gaaatctact ggtggcagga tatattgtgg tgtaaacaat
      841 ggagaaaaag attaattaag tactagtagc ctggtcgaaa cggtctcagt caggagagag
      901 acgaaaagca aaaacccgcc gaagcgggtt actagcgcca ttcgccattc agagagcgga
      961 gctgctgcga cggacgatcg gtacgcgcct cttcgctatt acgccaactg gcgaaaggtg
     1021 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     1081 gtaccacggc aaggctatct gtaatcattg ttgtactccg gttaggacgg attgggaact
     1141 ggctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1201 cctaatgagt gacgtctctc gctggagacc ggacaaggat gcgagctagc ctgcaggaat
     1261 tgttgatttt gtgatgactg atggcaggat atatgcggtt gtaattcatt tttattgtct
     1321 aaatttctgt atttgtttgt ttgttcggtt gtaaattttt ttggaagaac aagaaaagaa
     1381 aaaacacccg ttagggtgtt tttagttagt gtggcgcgcc gacttgcgac atgcggtcct
     1441 ttgttatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa
     1501 ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc
     1561 aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc
     1621 cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac
     1681 atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag
     1741 cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac
     1801 caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt
     1861 ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc
     1921 gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac
     1981 agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa
     2041 agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact
     2101 gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg
     2161 ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat
     2221 caccgcttcc ctcatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc
     2281 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     2341 ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     2401 gccgttacca ccgctgcgtt cggtcaaggt tcgatcggat tggcggttat gcggttctac
     2461 cggcgcggca gcgttacccg tgtcggcggc tccaacggct cgccatcgtc cagaaaacac
     2521 ggctcatcgg gcatcggcag gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc
     2581 aaggctggca ggtctggttc catgcccgga atgccgggct ggctgggcgg ctcctcgccg
     2641 gggccggtcg gtagttgctg ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca
     2701 tgccccaaca gcgattcgtc ctggtcgtcg tgatcaacca ccacggcggc actgaacacc
     2761 gacaggcgca actggtcgcg gggctggccc cacgccacgc ggtcattgac cacgtaggcc
     2821 gacacggtgc cggggccgtt gagcttcacg acggagatcc agcgctcggc caccaagtcc
     2881 ttgactgcgt attggaccgt ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg
     2941 ctgaccacca cggcgttctg gtggcccatc tgcgccacga ggtgatgcag cagcattgcc
     3001 gccgtgggtt tcctcgcaat aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc
     3061 acccagtgac cgggcttgtt cttggcttga atgccgattt ctctggactg cgtggccatg
     3121 cttatctcca tgcggtaggg gtgccgcacg gttgcggcac catgcgcaat cagctgcaac
     3181 ttttcggcag cgcgacaaca attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt
     3241 ctttaaccta cgcaatgagc tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc
     3301 ttttttaagt tgttgatttt taagtctttc gcatttcgcc ctatatcta
//
