LOCUS       sequence-318487-        3167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term Myc 3C cleavable Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 16 pCOLI_A_Myc
FEATURES             Location/Qualifiers
     source          1..3167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      484..523
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      554..1142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1043..1062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1296..1313
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1328..1467
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1393..1412)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1553..1575
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1569..1760)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     1997..2016
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1997..2015
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2053..2075
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2095..2112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2125..2154
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2161..2190
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2197..2226
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2233..2262
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2269..2298
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2305..2334
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2353..2376
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(2569..2587)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2583..2630
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2695..2786
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agtgataaca ctgcggcgaa cttacttctg acaaccatcg gtggaccgaa ggaattaacc
       61 gcttttttgc acaatatggg agaccatgta actcgccttg accgttggga accagaactg
      121 aatgaagcca taccaaacga cgagcgagac accacaatgc ctgcggcaat ggcaacaaca
      181 ttacgcaaac tattaactgg cgaactactt actctggctt cacggcaaca attaatagac
      241 tggcttgaag cggataaagt tgcaggacca ctactgcgtt cggcacttcc tgctggctgg
      301 tttattgctg ataaatctgg ggcaggagag cgtggttcac ggggtatcat tgccgcactt
      361 ggaccagatg gtaagccttc ccgtatcgta gttatctaca cgacgggtag tcaggcaact
      421 atggacgaac gaaatagaca gattgctgaa ataggggctt cactgattaa gcattggtaa
      481 accgatacaa ttaaaggctc cttttggagc cttttttttt ggacggaccg gtagaaaaga
      541 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      601 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      661 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
      721 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      781 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      841 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      901 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      961 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1021 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1081 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1141 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1201 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1261 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1321 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg
     1381 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     1441 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     1501 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     1561 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc
     1621 tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg
     1681 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg
     1741 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catggggtaa
     1801 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     1861 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     1921 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ggcaccatgc
     1981 atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctccggc
     2041 tccggaaata attttgttta actttaagaa ggagatatac atatgggaag ttctcatcac
     2101 caccatcatc atagttcggg ctccgagcag aaacttatct ctgaggaaga cttaaatggg
     2161 gagcagaagt tgatctcgga ggaagatctg aatggtgagc aaaagttaat ctccgaagaa
     2221 gacttgaatg gcgaacaaaa actgatctca gaggaagatt tgaacggcga acagaagtta
     2281 atctctgagg aggatctgaa cggagaacag aaacttattt cggaagaaga tttgaacggg
     2341 tcgagtggta gtctggaagt tctgttccag gggcccggat cccggtccga catatgagcg
     2401 cgcggaattc aaaggcctac gtcgacgagc tcactagtcg cggccgcttt cgaatctaga
     2461 gcctgcagtc tcgacaagct tgtcgagaag tactagagga tgtacaacgc gtgcgcgctg
     2521 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     2581 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     2641 gaactatatc gggattagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
     2701 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2761 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2821 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctc gtgaaagtaa
     2881 aagacgcaga ggaccaattg ggggcacgag tgggatacat agaactggac ttgaatagcg
     2941 gtaaaatcct tgagagtttt cgccctgaag agcgttttcc aatgatgagc actttcaaag
     3001 ttctgctatg tggagcagta ttatcccgtg tagatgcggg gcaagagcaa ctcggacgac
     3061 gaatacacta ttcgcagaat gacttggttg aatactcccc agtgacagaa aagcacctta
     3121 cggacggaat gacggtaaga gaattatgta gtgccgccat aacgatg
//
