LOCUS       sequence-318509-        7062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 1 luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC1CB0453
FEATURES             Location/Qualifiers
     source          1..7062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..632
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    724..748
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    1028..1083
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             1085..2734
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1151..1169)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             2798..2821
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2832..3539
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    3588..3612
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(3768..4916)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(5252..5840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5332..5351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6011..6802,6803..6871))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6634..6653
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6872..6976)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc
       61 acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa
      121 agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc
      181 ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct
      241 caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc
      301 gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg
      361 cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc
      421 cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc
      481 atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc
      541 tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc
      601 cagcggcgag ggcaaccagc ccggtgagcg tcgcaaagga gatcctgatc tgactgatgg
      661 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
      721 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
      781 gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtga agacaatgcc
      841 gaattcggat ccggagggag gcgtgtcgtt ttagtgaggt tatgaccccc gccgatgacg
      901 cgggattatg acccccgccg atgacgcggg attatgaccc ccgccgatga cgcgggacta
      961 tataaggttt tgctattcat tgaaagcagt agtgactgat ttgtatataa tgtactgtat
     1021 ttttacaaca attaccaaca acaacaaaca acaaacaaca ttacaattac tatttacaat
     1081 tacaatggag gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga
     1141 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
     1201 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat
     1261 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
     1321 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
     1381 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg
     1441 catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
     1501 gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca
     1561 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
     1621 cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc
     1681 tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt
     1741 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
     1801 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
     1861 atttcgagtc gtcttaatgt atagatttga agaagaactg tttctgagga gccttcagga
     1921 ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac
     1981 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct
     2041 ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg
     2101 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     2161 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     2221 gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc
     2281 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     2341 ttctggagac atagcttact gggacgagga cgaacacttc ttcatcgttg accgcctgaa
     2401 gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct
     2461 ccaacacccc aacatcttcg acgcaggtgt cgcaggtctg cccgacgatg acgccggtga
     2521 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     2581 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     2641 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     2701 cataaaggcc aagaagggcg gaaagatcgc cgtgggttcg gattataagg accatgacgg
     2761 agactataag gaccatgacc tcgacgctgc agcagcggat tataaggacg atgacgataa
     2821 gtgagcttgt cctgctttaa tgagatatgc gagaagccta tgatcgcatg atatttgctt
     2881 tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc
     2941 ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta tgaataatat
     3001 tctccgttca atttactgat tgtaccctac tacttatatg tacaatatta aaatgaaaac
     3061 aatatattgt gctgaatagg tttatagcga catctatgat agagcgccac aataacaaac
     3121 aattgcgttt tattattaca aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta
     3181 aaagactgat tacataaatc ttattcaaat ttcaaaagtg ccccaggggc tagtatctac
     3241 gacacaccga gcggcgaact aataacgctc actgaaggga actccggttc cccgccggcg
     3301 cgcatgggtg agattccttg aagttgagta ttggccgtcc gctctaccga aagttacggg
     3361 caccattcaa cccggtccag cacggcggcc gggtaaccga cttgctgccc cgagaattat
     3421 gcagcatttt tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga
     3481 cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg
     3541 accgctgcaa ttgtcttctg cacgaagtgg tttaaactat cagtgtttga caggatatat
     3601 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     3661 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttccccagat
     3721 caggcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa
     3781 tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg
     3841 caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg
     3901 aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac
     3961 atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg
     4021 tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc
     4081 ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg
     4141 cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc
     4201 gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag
     4261 gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt
     4321 tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg
     4381 tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac
     4441 agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata
     4501 tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc
     4561 tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc
     4621 gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttca
     4681 agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc
     4741 acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag
     4801 gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa
     4861 aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac
     4921 cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac
     4981 ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag
     5041 accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc
     5101 gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg
     5161 aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggatctgcca
     5221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5881 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5941 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6001 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6061 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6121 catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct ccagatttat
     6181 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6241 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6301 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6361 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6421 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6481 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6541 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6601 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6661 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6721 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6781 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6841 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6901 tttatcaagg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6961 aaataggggt tccgcgcacg aattggccag cgctgccatt tttggggtga ggccgttcgc
     7021 ggccgagggg cgcagcccct ggggggatgg gaggcccgcg tt
//
