LOCUS       sequence-318512-        2326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0417
FEATURES             Location/Qualifiers
     source          1..2326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     161..179
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(183..226)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      528..585
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(609..626)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(780..1368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(860..879)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1551..1645)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1666..2325)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2240..2259
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
       61 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
      121 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
      181 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcggtctc
      241 aggagggagg cgtgtcgttt tagtgaggat tgcgataaag gaaaggcatg aagataagat
      301 aataatgttg aagataagac tgctaggagg acctgacgta agggatgacg cacaggaaag
      361 attgatgaaa agtcaaaaac aaaaatcaat tatgtaatcc ttaccgctat gggtaagatt
      421 acctcacatg taggctatct tttcaacaag gctcactgct atataaggtt ttgctattca
      481 ttgaaagcag tagtgactga tttgtatata tactcgagac cctgcagtcc ggcaaaaaag
      541 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
      601 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      661 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      721 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      781 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      841 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      901 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      961 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1021 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1081 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1141 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1201 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1261 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1321 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1381 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1441 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1501 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
     1561 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1621 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1681 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1741 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1801 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1861 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     1921 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1981 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     2041 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     2101 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2161 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     2221 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2281 ttgggatata tcaacggtgg tatatccagt gatttttttc tccatt
//
