LOCUS       sequence-31852-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  TITLE     A. Sancar lab plasmid
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pfmh-hCry1
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             313..336
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             340..369
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             385..402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(425..442)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    431..655
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gaacagatct atcagcagct ttcacgatat agaggactag gtcttctggc atcagtacct
       61 tctaatccta atgggaatgg aggcttcatg ggatattctg cagaaaatat cccaggttgt
      121 agcagcagtg gaagttgctc tcaagggagt ggtattttac actatgctca tggcgacagt
      181 cagcaaactc acctgttgaa gcaaggaaga agctccatgg gcactggtct cagtggtggg
      241 aaacgtccta gtcaggaaga ggacacacag agtattggtc ctaaagtcca gagacagagc
      301 actaattcta gagactacaa agacgatgac gacaagttcg aacaaaaact catctcagaa
      361 gaggatctga atatgcatac cggtcatcat caccatcacc attgagttta aacccgctga
      421 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      481 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
      541 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
      601 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
      661 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
      721 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctag
      781 cgccgtcttc
//
