LOCUS       sequence-318520-       13263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express fulllength of mouse p150 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Cheng L, Zhang X, Wang Y, Gan H, Xu X, Lv X, Hua X, Que J, Ordog T, 
            Zhang Z
  TITLE     Chromatin Assembly Factor 1 (CAF-1) facilitates the establishment of
            facultative heterochromatin during pluripotency exit.
  JOURNAL   Nucleic Acids Res. 2019 Dec 2;47(21):11114-11131. doi: 
            10.1093/nar/gkz858.
  PUBMED    31586391
REFERENCE   2  (bases 1 to 13263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-12-2- 2"; volume: "47"; issue: 
            "21"; pages: "11114-11131"
COMMENT     Sequence Label: pWPXL-p150
FEATURES             Location/Qualifiers
     source          1..13263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5324..5335
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     regulatory      6290..6299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6296..6298,6299..6301,6302..7012)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6296..6298,6299..6301,6302..7012)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6341..6362)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6602..6621)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6949..6970
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7094..7682
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7147..7167)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7565..7576)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7826..7859)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7879..8059
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8084..8102)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8085..8102)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8392..8411)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8625..8644)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8744..8766
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8804..8822)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8890..8994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8995..9063,9064..9855)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9213..9232)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10026..10614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10515..10534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10768..10785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10855..10875)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10860..11189
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11040..11175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12323..12388
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12518..12538
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12963..13097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13000..13019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13054..13073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttaaac tacgggatcc cgatgttgga
     3541 ggagccggag gccgcgacgc ggacagccgc ggccgtggat tgcaaagaca gacccgggtt
     3601 cccagtgaag aggttaatac aagcccgctt gcccttcaaa cgcctgaacc ttgttcccaa
     3661 ggagaaagtt gaggaagaca catcaccaaa agcagctgtg gaaagcaaag tcccggatct
     3721 acaattgtct ttgggcactt ttgaaagcca gtgtcacaca ggctctcacg taggcttgag
     3781 tacaaagttg gtcggtggcc agggccccat tgatagcttc ttgagggcaa caatcaagcc
     3841 agtaccgagt gtggtcatta tcgacctgac ggagaactgc agtgacattc cagacagccc
     3901 tgagggccac agtgaactaa gtcctgacac tgctggtgtg gtcaccacag tggaaggagc
     3961 tgccaagcag caggagcact ctgcagcaga gctgtgtctc ctagagaccc cttcagacat
     4021 cacttgtcac atggaagagg agcctgggag tccaggagac ccaaagagga cgggtgactg
     4081 tcaggctggt tcactacaga gctgtccgga gctgacccca ggttctagga catgccccac
     4141 caaggagtta tcaagctgga gcaaggcagg ggatctccta tttatagaga aagtgcctgt
     4201 ggtggtgcta gaggacatcc tggccactaa gccctccatt gcctctcttc ccatgatgtc
     4261 cctggatagg agtgtcactt cagagagtga aatactggag tcctgccccg aggatgactc
     4321 catactgagc cactcctcca ccaactcctc ttctccaacc agctctcctg agggaccatc
     4381 cacaccccca gaacatcggg gtgggagaag ctccccctcc accccagcct gcagagttgc
     4441 caagaacttt gtcaaaggct ccacagagaa gggcaggagc aaactgcaca gagatagaga
     4501 acagcagagg gaagagaagg aaaagctgag ggaagagata cgacgagcca aggaggaggc
     4561 acggaagaag aaggaggagg agaaggagct gaaggaaaag gagcggaggg aaaagcggga
     4621 gaaagatgag aaggagaagg cggagaagca gcggctaaag gaggaaaaac gcaaggagcg
     4681 gcaggaagct ctggaggcta aactggagga gaagaggaag aaggaggaag agaagcggtt
     4741 acgggaggaa gagaagcggt tacgggaaga agagaagcgc attaaggcag aaaaggcaga
     4801 gatcactcga ttcttccaga aaccgaagac tccgcaggct cccaagaccc tggccggttc
     4861 ctgtggtaag tttgccccct ttgaaatcaa agaacacatg gtgctggctc cacggtgccg
     4921 agctgccttg gatcaagacc tgtgcgatca gctggaccag ctcctgcagc agcagagtgt
     4981 ggcaagcacc ttcctcagtg acctaaaaag ccggctgccc ttgagatcgg ggcccacccg
     5041 ggtctgtggc catgacacag acatcatgaa cagggatgtg gtcattgtgg agagcagcaa
     5101 ggtggacggt gtgtctgaaa ggaagaagtt tggccgcatg aagcttctgc agttctcaga
     5161 gaaccaccgg ccagcatact gggggacgtg gaacaagaag acagccatca tccgcccccg
     5221 gaacccctgg gcccaggaca aagaccttct tgactatgag gtagacagtg acgatgagtg
     5281 ggaagaggag gaacctgggg agtctttgtc ccacagtgag ggggatgagg atgacgatgt
     5341 gggggaagat gaggatgagg acgatggctt ctttgtgccc catgggtact tatctgaaga
     5401 tgaaggcgtg actgaggagt gtgcagaccc tgagaaccac aaagtacacc agaagctgaa
     5461 ggccaaggag tgggatgagc tcctagccaa gggcaagcgc tttcgtgtgc tgcagcctgt
     5521 gcacgtggga tgtgtatggg catcagaggc agccaactgc acgagttccg acttgaagct
     5581 gctgcagcag ttcactgcat gcctgctgga tgtggcatcc cctgatgagc cagaacccgg
     5641 ggcctccagg agggaaaaga gggaccagca tatcctggcc cagttgcttc cgctgctaca
     5701 tggcaatgtg aatgggagca aggtgatcat ccatgaattc caggagcaat gccgcagggg
     5761 cctgctgacc cttccaagtc ccaccccaca cctgcagatg cccaacttgg aggatgctgt
     5821 tgctgtgcca tccaaggcca ggctgaagcg ccttatctct gagaactctg cctatgagaa
     5881 gcggcccaac ttccgtatgt gctggtatgt gcacccagag gtgctcaaga gctttggcca
     5941 ggagtgcctg ccagtgccct gccagtggac ctacatcacc accatgccct cagcacccag
     6001 ggaagacagt ggcagtgctt ctactgaggg gcctggccag agcacaccta tgctactcaa
     6061 gcggaagcca gcggccacca tgtgcatcac gcagtttatg aagaagcggc gctatgatgg
     6121 acaggttggg tctggggaca tggatggctt ccaggcagac acagaagagg atgaagagga
     6181 tgacacagac tgtatgatca tagatgtgcc agatgttggg agtgacgtgt cagaagctcc
     6241 catacctgct cccacacttt gcaaggcacg cgtcctagcg ctaccggtcg ccaccatggt
     6301 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     6361 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     6421 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     6481 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     6541 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     6601 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     6661 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     6721 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     6781 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     6841 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     6901 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     6961 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
     7021 cagatctcga ctagctagta gctagctagc tagtcgagct caagcttcga attcccggga
     7081 ctagtcatat gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7141 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7201 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7261 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7321 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7381 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7441 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7501 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7561 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7621 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7681 gcatcggtac gtatggccag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7741 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7801 aagatgggat caattcacca tgggaataac ttcgtatagc atacattata cgaagttatg
     7861 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7921 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7981 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8041 gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa
     8101 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata
     8161 tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt
     8221 aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc
     8281 cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg
     8341 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     8401 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     8461 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     8521 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     8581 tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat
     8641 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     8701 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     8761 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     8821 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     8881 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8941 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9001 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9061 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9121 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9181 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     9241 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9301 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     9361 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     9421 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     9481 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     9541 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9601 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9661 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9721 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9781 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9841 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9901 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9961 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10021 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10081 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10141 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10201 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10261 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10321 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10381 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10441 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10501 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10561 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10621 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10681 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    10741 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    10801 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg
    10861 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10921 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
    10981 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
    11041 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    11101 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    11161 gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata
    11221 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
    11281 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
    11341 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
    11401 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
    11461 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt
    11521 tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc
    11581 acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca
    11641 tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc
    11701 caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact
    11761 atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat
    11821 tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc
    11881 tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg
    11941 acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg
    12001 acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt
    12061 aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt
    12121 gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc
    12181 gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg
    12241 gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
    12301 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
    12361 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
    12421 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
    12481 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
    12541 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
    12601 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
    12661 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
    12721 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
    12781 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
    12841 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
    12901 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
    12961 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    13021 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    13081 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    13141 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    13201 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    13261 tag
//
