LOCUS       sequence-318535-        5171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term 3C cleavable His-HA tag for insect cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 59 pLIB_HIS_HA
FEATURES             Location/Qualifiers
     source          1..5171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(298..1089,1090..1158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     921..940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1159..1263)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1289..1743)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1426..1447)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1637..1656
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  1927..2092
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    2202..2336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2226..2245)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2280..2299
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2565..2588)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(2601..2627)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2634..2660)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2667..2693)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2706..2735)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(2709..2738)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(2712..2741)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(2796..2887)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(2854..2871)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     protein_bind    2935..2968
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(3364..3369,3370..3386,3387..3392)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3581..4114
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSIGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  4181..4405
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(4539..4556)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4710..5171,1..127))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4790..4809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
       61 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      181 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      241 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      301 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      361 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      421 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      481 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      541 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      601 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      661 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      721 taattccttc ggtccaccga tggttgtcag aagtaagttg gccgcagtgt tatcactcat
      781 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      841 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      901 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      961 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1021 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1081 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1141 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1201 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1261 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     1321 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     1381 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     1441 agtccactat taagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     1501 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     1561 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     1621 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     1681 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     1741 cgtcccattc gccattcagg ctgcaaataa gcgttgatat tcagtcaatt acaaacatta
     1801 ataacgaaga gatgacagaa aaattttcat tctgtgacag agaaaaagta gccgaagatg
     1861 acggtttgtc acatggagtt ggcaggatgt ttgattaaaa acataacagg aagaaaaatg
     1921 ccccgctgtg ggcggacaaa atagttggga actgggaggg gtggaaatgg agtttttaag
     1981 gattatttag ggaagagtga caaaatagat gggaactggg tgtagcgtcg taagctaata
     2041 cgaaaattaa aaatgacaaa atagtttgga actagatttc acttatctgg ttcggatctc
     2101 ctaggggtta tgatagttat tgctcagcgg tggcagcagc caactcagct tcctttcggg
     2161 ctttgttagc agccggatct tctaggctca agcagtgatc agatccagac atgataagat
     2221 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2281 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2341 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     2401 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagta cttctcgaca
     2461 agcttgtcga gactgcaggc tctagattcg aaagcggccg cgactagtga gctcgtcgac
     2521 gtaggccttt gaattccgcg cgctcatatg tcggaccggg atccgggccc ctggaacaga
     2581 acttccaggg aacctgaact tgcatagtct ggcacatcgt atggataaga tcccgcataa
     2641 tcggggacat cgtaaggata gctaccagca tagtctggca cgtcatacgg ataggagcca
     2701 gagctgtgat gatgatgatg atggtgatga tgatgatgat ggctgctgcc catagatccg
     2761 cgcccgatgg tgggacggta tgaataatcc ggaatattta taggtttttt tattacaaaa
     2821 ctgttacgaa aacagtaaaa tacttattta tttgcgagat ggttatcatt ttaattatct
     2881 ccatgatcta ttaatattcc ggagtaggtc gcgaatcgat actagtaaaa ccagataact
     2941 tcgtataatg tatgctatac gaagttatgg taccgcggcc gcgtagagga tctgttgatc
     3001 agcagttcaa cctgttgata gtacaaacaa agctgtagcc aaccactaga actatagcta
     3061 gagtcctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg aaccacttca
     3121 tccggggtca gcaccaccgg caagcgccgc gacggccgag gtctaccgat ctcctgaagc
     3181 aagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc caatgcctga
     3241 cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact
     3301 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacaaacc
     3361 cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat gcgctcacgc
     3421 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     3481 gcttgttatg actgtttttt tgtacagtct atgcctcggg catccaagca gcaagcgcgt
     3541 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagcaacga
     3601 tgttacgcag cagggcagtc gccctaaaac aaagttaggt ggctcaagta ttggcatcat
     3661 tcgcacatgt aggctcggcc ctgaccaagt caaatccatg cgggctgctc ttgatctttt
     3721 cggtcgtgag ttcggagacg tagccaccta ctcccaacat cagccggact ccgattacct
     3781 cgggaacttg ctccgtagta agacattcat cgcgcttgct gccttcgacc aagaagcggt
     3841 tgttggcgct ctcgcggctt acgttctgcc caagtttgag cagccgcgta gtgagatcta
     3901 tatctatgat ctcgcagtct ccggcgagca ccggaggcag ggcattgcca ccgcgctcat
     3961 caatctcctc aagcatgagg ccaacgcgct tggtgcttat gtgatctacg tgcaagcaga
     4021 ttacggtgac gatcccgcag tggctctcta tacaaagttg ggcatacggg aagaagtgat
     4081 gcactttgat atcgacccaa gtaccgccac ctaacaattc gttcaagccg agatcggctt
     4141 cccggccgcg gagttgttcg gtaaattgtc acaacgccgc gaatatagtc tttaccatgc
     4201 ccttggccac gcccctcttt aatacgacgg gcaatttgca cttcagaaaa tgaagagttt
     4261 gctttagcca taacaaaagt ccagtatgct ttttcacagc ataactggac tgatttcagt
     4321 ttacaactat tctgtctagt ttaagacttt attgtcatag tttagatcta ttttgttcag
     4381 tttaagactt tattgtccgc ccacacccgc ttacgcaggg catccattta ttactcaacc
     4441 gtaaccgatt ttgccaggtt acgcggctgg tctatgcggt gtgaaatacc gcacagatgc
     4501 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4561 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4621 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4741 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aatagtattt
     5161 ggtatctgcg c
//
