LOCUS       sequence-318536-        5081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term uncleavable CBP tag for insect cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 57 pLIB_C_CBP
FEATURES             Location/Qualifiers
     source          1..5081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(634..639,640..656,657..662))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1058..1091)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1139..1230
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1155..1172
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1393..1470
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     polyA_signal    1600..1734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1637..1656)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1691..1710
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1844..2009
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2193..2647
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2280..2299)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2489..2510
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2673..2777
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2778..2846,2847..3638)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2996..3015)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3809..4397
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4298..4317
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4551..4568
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4702..4926
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(4993..5081,1..445))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
       61 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
      121 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
      181 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
      241 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
      301 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
      361 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
      421 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
      481 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
      541 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
      601 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
      661 aactgggttt gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
      721 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg
      781 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg
      841 cccttgcttc aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc
      901 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag
      961 gactctagct atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa
     1021 ctgctgatca acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta
     1081 tacgaagtta tctggtttta ctagtatcga ttcgcgacct actccggaat attaatagat
     1141 catggagata attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt
     1201 aacagttttg taataaaaaa acctataaat attccggatt attcataccg tcccaccatc
     1261 gggcgcggat cccggtccga catatgagcg cgcggaattc aaaggcctac gtcgacgagc
     1321 tcactagtcg cggccgcttt cgaatctaga gcctgcagtc tcgacaagct tggaagcgga
     1381 tctggatctg gaaagcgacg atggaaaaag aatttcatag ccgtctcagc agccaaccgc
     1441 tttaagaaaa tctcatcctc cggggcactt taataagtcg agaagtacta gaggatcata
     1501 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     1561 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     1621 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1681 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca
     1741 ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     1801 tgccaccgct gagcaataac tatcataacc cctaggagat ccgaaccaga taagtgaaat
     1861 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
     1921 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
     1981 ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta
     2041 atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt tttctctgtc
     2101 acagaatgaa aatttttctg tcatctcttc gttattaatg tttgtaattg actgaatatc
     2161 aacgcttatt tgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     2221 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2281 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2341 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2401 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2461 tttgacgttg gagtccacgt tcttaatagt ggactcttgt tccaaactgg aacaacactc
     2521 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2581 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     2641 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2701 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2761 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2821 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2881 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2941 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3001 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3061 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3121 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3181 tacttctgac aaccatcggt ggaccgaagg aattaaccgc ttttttgcac aacatggggg
     3241 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3301 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3361 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3421 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3481 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3541 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3601 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3661 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3721 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3781 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3841 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3901 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actattcttc
     3961 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4021 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4081 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4141 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4201 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4261 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4321 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4381 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4441 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4501 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4561 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4621 tttcacaccg catagaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
     4681 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
     4741 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
     4801 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
     4861 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ccaagggcat ggtaaagact
     4921 atattcgcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc
     4981 ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg
     5041 tatgcccaac tttgtataga gagccactgc gggatcgtca c
//
