LOCUS       sequence-31854-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Uttamapinant C, White KA, Baruah H, Thompson S, Fernandez-Suarez M, 
            Puthenveetil S, Ting AY
  TITLE     A fluorophore ligase for site-specific protein labeling inside 
            living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 15. 107(24):10914-9.
  PUBMED    20534555
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Jun 15. 107(24):10914-9."
COMMENT     Sequence Label: pcDNA3-LplA(W37V) Coumarin PRIME ligase
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             118..135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             139..171
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(175..183,184..198)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             205..228
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttggtttagc gactctctgg ctaactagag aacccactgc ttactggctt atcgaaatta
       61 atacgactca ctatagggag acccaagctt gcggccgcca ccatggtgcg gggttctcat
      121 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
      181 tacgacgatg acgataagga tccagactac aaggatgacg acgataagat gagcaccctg
      241 agactgctga tcagcgacag ctacgacccc tggttcaacc tggccgtgga ggagtgcatc
      301 ttcagacaga tgcccgccac ccagagagtg ctgttcctgg tgagaaacgc cgacaccgtg
      361 gtgatcggca gagcccagaa cccctggaag gagtgcaaca ccagaagaat ggaggaggac
      421 aacgtgagac tggccagaag aagcagcggc ggcggcgccg tgttccacga cctgggcaac
      481 acctgcttca ccttcatggc cggcaagccc gagtacgaca agaccatcag caccagcatc
      541 gtgctgaacg ccctgaacgc cctgggcgtg agcgccgagg ccagcggcag aaacgacctg
      601 gtggtgaaga ccgtggaggg cgacagaaag gtgagcggca gcgcctacag agagaccaag
      661 gacagaggct tccaccacgg caccctgctg ctgaacgccg acctgagcag actggccaac
      721 tacctgaacc ccgacaagaa gaagctggcc gccaagggca tcaccagcgt gagaagcaga
      781 gtgaccaacc tgaccgagct gctgcccggc atcacccacg agcaggtgtg cgaggccatc
      841 accgaggcct tcttcgccca ctacggcgag agagtggagg ccgagatcat cagccccaac
      901 aagacccccg acctgcccaa cttcgccgag a
//
