LOCUS       sequence-31855-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Uttamapinant C, White KA, Baruah H, Thompson S, Fernandez-Suarez M, 
            Puthenveetil S, Ting AY
  TITLE     A fluorophore ligase for site-specific protein labeling inside 
            living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 15. 107(24):10914-9.
  PUBMED    20534555
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Jun 15. 107(24):10914-9."
COMMENT     Sequence Label: pcDNA3-LplA(W37I) Alternative coumarin PRIME l...
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..122
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             126..158
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(162..170,171..185)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             192..215
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gagattaata cgactcacta
       61 tagggagacc caagcttgcg gccgccacca tggtgcgggg ttctcatcat catcatcatc
      121 atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg
      181 ataaggatcc agactacaag gatgacgacg ataagatgag caccctgaga ctgctgatca
      241 gcgacagcta cgacccctgg ttcaacctgg ccgtggagga gtgcatcttc agacagatgc
      301 ccgccaccca gagagtgctg ttcctgatca gaaacgccga caccgtggtg atcggcagag
      361 cccagaaccc ctggaaggag tgcaacacca gaagaatgga ggaggacaac gtgagactgg
      421 ccagaagaag cagcggcggc ggcgccgtgt tccacgacct gggcaacacc tgcttcacct
      481 tcatggccgg caagcccgag tacgacaaga ccatcagcac cagcatcgtg ctgaacgccc
      541 tgaacgccct gggcgtgagc gccgaggcca gcggcagaaa cgacctggtg gtgaagaccg
      601 tggagggcga cagaaaggtg agcggcagcg cctacagaga gaccaaggac agaggcttcc
      661 accacggcac cctgctgctg aacgccgacc tgagcagact ggccaactac ctgaaccccg
      721 acaagaagaa gctggccgcc aagggcatca ccagcgtgag aagcanagtg accaacctga
      781 ccgagctgct gcccggcatc acccacgagc aggtgtgcga ggccatcacc gaggccttct
      841 tcgcccacta ccgcgagaaa gtggaggccg agatcatcag ccccnaacaa gaaccccgaa
      901 cttgcccaac ttcgccgaga ccttccccag gacagagcag ctgggaagtg gaacttcggg
      961 ccaggccccc ggccttcacc acctg
//
