LOCUS       sequence-318555-       11128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_PflaBS-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..11128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        3661..3736
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(5179..5193,5194..5199,5200..5216,5217..5223)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    5199..5215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5231..5247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5266..5277
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(5284..5310,5311..5313,5314..7800,7801..7803,
                     7804..9390)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9560..10351
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10474..11062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10963..10982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcactag atccccggca agttcatcct tagggttttc atatctctta aaaagtcaat
       61 ttctgcttga ttaatttctt gtggattatt gtttttgcgg tttttcattt tttatctcct
      121 tgagtattga attttttctt atatataaat atatagcaaa aactattttt gccaactttt
      181 ttacaaaaaa ttttttatta aaacacttag ggctttgcta aattctcttt taaaagaact
      241 tagtaaagcc ctatggattt aagaactgat tatacttacg tagtaaaaat actacagata
      301 ttaatagtat agaccatatc aaaatcataa tcaatttata tttatctaaa attgtagtcc
      361 atacaaaata caaaaaaatt aggtttttct aaattctatt ttaaaagaac ttagtaacac
      421 ctaatctcta aattacttac aaaattctaa aattagattt ttagttcttc atatttcttt
      481 aaaagttttt taagaaaatc tttttgattt tcaaaaattt catgcatcat aaaactagta
      541 aatttggatt tttttttgta aaaatcataa ctttcttggg ttttaagttg aaatcttaat
      601 ggttttattg gattttgcct agattttttt aacgccggac tttctttatc ttttataaaa
      661 ttataagaat ttttaatacc attttctatt aaataatctt cttttataag cccctcttgc
      721 agtgcagttg ctaatcttaa atacttatac gcttgagttt ttgctacatc aaagtctttt
      781 ataaaatcaa gaaaagtttt ataaccatca aatttataat attgattatc ttttatttct
      841 tttaaaattt taatattttc caccctgtgg aatatttctt ttttgaaatt gtcatttaac
      901 ttttccttta gtgtcttgta ataagaaatt ttactttcat taatcattaa attttgttca
      961 acataagtat ctatcttttt tgttaaaact ttaacaacac gttttttttc cataaaaatt
     1021 ttcctttatt tttgcaacat tttccttaat tgatataatt gttactattt tagatttttt
     1081 aatatccact taagaattaa tccctcacta gaatattttt tattatagaa ttcatataca
     1141 tgtttttcta aatctataaa tttatcaaca attgctttat tatagggtgt atctatcttt
     1201 tcttttctta ataatcgtct catattcttt aaataacaac aaacactgcc ggttttaaac
     1261 ctaaattcta tgtaatatat ttttgaaaaa gtatatacat tttcaacccc gtttatttca
     1321 tactttacaa aaacattatt tattggtttt ctataaccat agtaaatgcc taaaaatttg
     1381 tccccgtctt tcataggata tagataaagt ttttgaatct ctgatttatc taataatttt
     1441 tcaaaaaaaa ttatacaccg cttttgtttg ttactaacta ctctatgcaa tgcctttaat
     1501 atttttgtgt gatatatatt tttatcacct tttttttctt tcagcaggaa gatactttct
     1561 tttggaaatt taattttttg ttcatttatt cttgcagcct taagtccgat tttcatattt
     1621 aatccttata tagcctttct atttaattct tttaaactca ataaactttt ttggtttttc
     1681 attagttcta ataattcgca ataatatgtg caaaacactt tattgtattc tattttcttt
     1741 tggttattca aatattcttt tataattggc tttagaagtt caatatttac ttcttttttt
     1801 agttgttcaa taaggatatt gaaaatgttt gtttttaaat cttgataatt ttgttgagaa
     1861 ttttcttttt ttctttcaat tgacttctct aatttacgct ttatattgtt taaatcgcta
     1921 tatttatgac tttcaataat aaaatgaggc ttaaatttgt aattttcgta tattttttgg
     1981 aaacttgttt ctaattgttt agaattatat tcattttttt caaattcttt ttgagtgttg
     2041 catagaattt cctttaattt ttcttgtttt tctttcatac aagatttagt aaaatgtata
     2101 tttttatttt ttagtaattt aatttctatt atttttatga ttttaagtat tttgattata
     2161 ttatctttat caatatttaa attgaataaa attgtaagaa aaattgaaag aattttttta
     2221 cataaaaagt tacatttatt gaagtaattt cttacttgat acttttctat ttcattaatc
     2281 tttctttctt cttttatatt atttttatta tttaaacact cctttaaatc tacactccca
     2341 tttttagtaa atctatcttt aaggcccctt gaaactctat tttgaaatct taaatctttt
     2401 ttttcttgaa agtatttata gattttttgg taacattctt tttttgaaaa attaagttga
     2461 tagtatattt cggtccccaa atttacccct aagtgtttgt gataattagt tgtgacttta
     2521 aattcttttt ctaatttgta aaggtaattt tgcagtgttt ttagtttaac gggtgtttga
     2581 ctatttcttt taaggttttc attaaagtaa tagagtatgt ttttttgtgt gtatttctta
     2641 tatgttttat ttatgtacgc tagtgttgag ataaggacta ctaatttata ctgtaattta
     2701 ttgtagcaag ttgtggtttt actggttttg tttatattag ataagttttc catttatagc
     2761 tccttgaata ccggattttt tatctataaa gtatatatat cacaattttg atttaaaagt
     2821 aaattctttt tttaaaaata caaaaatttt ttataaatta tatagaccat gatattgtat
     2881 taatactaat tgattttaat ttaaggttga actaaaataa acatagtttt gtagaaaata
     2941 atttttcatt attttgctac ttgatattgg atcgtaaaaa tattagggct ttacaaagtt
     3001 cttttaaaag agaatttagt aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa
     3061 gttggcaaaa atagtttttg ctatatattt atatataaga aaaaattcaa tactcaagga
     3121 gataaaaaat ggaaaactta aacaataatc cacaaaaaat taatcaaata aataagcaaa
     3181 ctaattttat tggttttgaa aattttatag aacttgataa tcaagttaat caaactaaag
     3241 aaaaaagtaa attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt
     3301 gcggccggaa acagctatga catgattacg aattcgagct ctgtctgtcg cctcttgtgg
     3361 cttccggagg agttatttat aataaaataa agaattacga cttgaaaatt tttaatcaaa
     3421 aaaataaagt tgaagattat aaaaagaagt acgaagatag agagagaaaa gtggaaggtg
     3481 aacttaatac cttggatttt accgttaagc gcatgaaaga tcaagaaaat acattaaagg
     3541 cttttgattt taatcaaaga aataaataat aataattatt tttaatgcta ttgctatttg
     3601 cgtttctttt tttttaattt ttgtgctatt ctttttaacg aaaatttaaa tttctgactt
     3661 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     3721 ggcaccgagt cggtgctttt tttggcgcgc caaataatgc ccgtgtccct gttgaatagg
     3781 gacacggttt tttaacaatt tttttattcc taggccttag tggccggtac ccggggatcc
     3841 ttattactgg ccgctttcta gcctggatac ttgtctggct agttgcatta gagagtcagc
     3901 tagagcccta gggctagctg tttgtgtatt cggagccagt gtagtttttc tttttactaa
     3961 cgatacgggt aataattgat tgccttttac agcctgacct tgagacaatt gcaaaagtct
     4021 gtccacagag gtttgaccta ataatctaaa atcttgtttg atagtagtaa gtggaggtat
     4081 gtagcaacta gaatcttctg tatcatcata acccacaact gaaatatcag ccccaactct
     4141 aagtccactt tcagtaatag ctcgcatagc tcctaaagcc atttgatcat tagcaactaa
     4201 catagcagta gggacaatgc cttcatttaa catttgcatt gtttgctgaa atcctgacat
     4261 agcagaccaa tctccttctc tttctgctat tggttgtatt tgatttcttg ttaaatactt
     4321 gtgccatcct gctaatctca atctggcact tactgaactt aagggacctg caagtagtgc
     4381 aatttgttga tggcctaatg caacaaggtg ttcaactcct aaccttgttc catcttcgtg
     4441 agaaaaaatt atagagttta ttggggtttg gtccgacaca tccaaaaaca atgctggaac
     4501 attagtacat gctgcttcaa ctgcaatcgc atcttggtca tcaagaggat aattaattat
     4561 aagtccacta acacgttgag ctaataaatt atgtactgct gccttacaag cctctacacc
     4621 tgatctctca accattgata caacaacgct tgcaccaagt tgatcagctc tcgatttaat
     4681 tgcggctaca atttgactag gcgcatgtaa tgcaagtgat gatgtggcaa ctccaattaa
     4741 aagtgattgt ttccccgcaa gttgctgagc aacgcgattt ggtatataat tcaactctgc
     4801 catagctgct tcaaccttct ctcttgtttt tgcagataca tgacttgctt ggttcacaac
     4861 tctcgaaacc gtttggtagc taaccccggc atattcagca acatcataca aagtaacagg
     4921 tttcatggat cattcctcca tgataaaatt taaatttctg actttggcaa atccttttgc
     4981 ctgttaaaaa gaatagcaca aaaattaaaa aaaaagaaac gcaaatagca atagcattaa
     5041 aaataattat tattatttat ttctttgatt aaaatcaaaa gcctttaatg tattttcttg
     5101 atctttcatg cgcttaacgg taaaatccaa ggtattaagt tcaccttcca cttttctctc
     5161 tctatcttct ctagaaaatc ataaaaaatt tatttgcttt gtgagcggat aacaattata
     5221 atagattcaa ttgtgagcgg ataacaattt cacacagaat tcattaaaga ggagaaatta
     5281 catatggata agaaatactc aataggctta gctatcggca caaatagcgt cggatgggcg
     5341 gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac
     5401 cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg
     5461 gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt
     5521 tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat
     5581 cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt
     5641 ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga
     5701 aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg
     5761 catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt
     5821 gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac
     5881 cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca
     5941 agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg
     6001 aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca
     6061 gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg
     6121 gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct
     6181 attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct
     6241 tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt
     6301 cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat
     6361 gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt
     6421 ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg
     6481 cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt gggtgagctg
     6541 catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag
     6601 attgaaaaaa tcttgacttt tcgaattcct tattatgttg gtccattggc gcgtggcaat
     6661 agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa
     6721 gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat
     6781 aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg
     6841 gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt
     6901 ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta
     6961 accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa
     7021 atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa
     7081 attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt
     7141 gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat
     7201 gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg
     7261 ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata
     7321 ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat
     7381 gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt
     7441 ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag
     7501 actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc
     7561 gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag
     7621 cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat
     7681 cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga
     7741 agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat
     7801 gccattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt
     7861 tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg
     7921 aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat
     7981 ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc
     8041 caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg
     8101 aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa
     8161 tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac
     8221 aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag
     8281 aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt
     8341 aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac
     8401 tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa
     8461 cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat
     8521 tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa
     8581 gtccagacag gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt
     8641 attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta
     8701 gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc
     8761 gttaaagagt tactagggat cacaattctg gaaagaagtt cctttgaaaa aaatccgatt
     8821 gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct
     8881 aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa
     8941 ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct
     9001 agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg
     9061 gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt
     9121 gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac
     9181 aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga
     9241 gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca
     9301 aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc
     9361 attgatttga gtcagctagg aggtgactaa taaaagcttg atcagatctc agcttttttt
     9421 tgaagtgcct ggcagtaagt tgaaaaaatt tgaaataaac ttggactatg ttaataaaaa
     9481 aatacaaatt aacgaacaaa aagatttgaa ttattatata atttagttaa aagcaatttt
     9541 aaatgaggga ggtttccata tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     9601 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     9661 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     9721 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     9781 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     9841 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     9901 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     9961 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
    10021 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
    10081 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
    10141 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
    10201 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
    10261 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
    10321 aggcgagatc accaaggtag tcggcaaata atgacgtcgc cggagcggtc gagttctgga
    10381 ccgaccggct cgggttctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    10441 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    10501 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    10561 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    10621 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    10681 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    10741 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    10801 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    10861 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    10921 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    10981 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    11041 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    11101 ttgctggcct tttgctcaca tgttcttt
//
