LOCUS       sequence-31856-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Uttamapinant C, White KA, Baruah H, Thompson S, Fernandez-Suarez M, 
            Puthenveetil S, Ting AY
  TITLE     A fluorophore ligase for site-specific protein labeling inside 
            living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 15. 107(24):10914-9.
  PUBMED    20534555
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Jun 15. 107(24):10914-9."
COMMENT     Sequence Label: pcDNA3-LAP2-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..24)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(27..47)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(51..71)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(90..800,801..803,804..806))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(132..153)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     481..500
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     740..761
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             878..892
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 gataaccttc ctcttcttct tggggtcacc ttcctcttct tcttggggtc caccttcctc
       61 ttcttcttgg ggtcagccct gctgaattcc ttgtatagct cgtccatgcc gagagtgatc
      121 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      181 ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      241 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
      301 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
      361 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      421 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      481 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
      541 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc
      601 aggccgtagc cgaaggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
      661 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
      721 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
      781 aacagctcct cgcccttgct caccatcgag ctcggatcct tggcgtccag gtcgtaccac
      841 accttgtcga tctcgaagcc gctagccata ccatgatgat gatgatgata aacccac
//
