LOCUS       sequence-318560-        6747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Block 1 for AdenoBuilder genome assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Miciak JJ, Hirshberg J, Bunz F
  TITLE     Seamless assembly of recombinant adenoviral genomes from high-copy 
            plasmids.
  JOURNAL   PLoS One. 2018 Jun 27;13(6):e0199563. doi: 
            10.1371/journal.pone.0199563. eCollection 2018.
  PUBMED    29949649
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-06-27"; volume: "13(6):e0199563. doi"; pages: " 
            10.1371/journal.pone.0199563. eCollection 2018"
COMMENT     Sequence Label: pAd5-B1
FEATURES             Location/Qualifiers
     source          1..6747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   379..481
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     569..719
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        complement(join(4579..4585,4586..4603,4604..4609))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    4624..4645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4765..4782)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4936..5524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5016..5035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5695..6486,6487..6555))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6318..6337
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6556..6660)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6728..6746
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcttcgaaca tcatcaataa tataccttat tttggattga agccaatatg
      421 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
      481 gtagtgtggc ggaagtgtga tgttgcaagt gtggcggaac acatgtaagc gacggatgtg
      541 gcaaaagtga cgtttttggt gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt
      601 tttaggcgga tgttgtagta aatttgggcg taaccgagta agatttggcc attttcgcgg
      661 gaaaactgaa taagaggaag tgaaatctga ataattttgt gttactcata gcgcgtaata
      721 tttgtctagg gccgcgggga ctttgaccgt ttacgtggag actcgcccag gtgtttttct
      781 caggtgtttt ccgcgttccg ggtcaaagtt ggcgttttat tattatagtc agctgacgtg
      841 tagtgtattt atacccggtg agttcctcaa gaggccactc ttgagtgcca gcgagtagag
      901 ttttctcctc cgagccgctc cgacaccggg actgaaaatg agacatatta tctgccacgg
      961 aggtgttatt accgaagaaa tggccgccag tcttttggac cagctgatcg aagaggtact
     1021 ggctgataat cttccacctc ctagccattt tgaaccacct acccttcacg aactgtatga
     1081 tttagacgtg acggcccccg aagatcccaa cgaggaggcg gtttcgcaga tttttcccga
     1141 ctctgtaatg ttggcggtgc aggaagggat tgacttactc acttttccgc cggcgcccgg
     1201 ttctccggag ccgcctcacc tttcccggca gcccgagcag ccggagcaga gagccttggg
     1261 tccggtttct atgccaaacc ttgtaccgga ggtgatcgat cttacctgcc acgaggctgg
     1321 ctttccaccc agtgacgacg aggatgaaga gggtgaggag tttgtgttag attatgtgga
     1381 gcaccccggg cacggttgca ggtcttgtca ttatcaccgg aggaatacgg gggacccaga
     1441 tattatgtgt tcgctttgct atatgaggac ctgtggcatg tttgtctaca gtaagtgaaa
     1501 attatgggca gtgggtgata gagtggtggg tttggtgtgg taattttttt tttaattttt
     1561 acagttttgt ggtttaaaga attttgtatt gtgatttttt taaaaggtcc tgtgtctgaa
     1621 cctgagcctg agcccgagcc agaaccggag cctgcaagac ctacccgccg tcctaaaatg
     1681 gcgcctgcta tcctgagacg cccgacatca cctgtgtcta gagaatgcaa tagtagtacg
     1741 gatagctgtg actccggtcc ttctaacaca cctcctgaga tacacccggt ggtcccgctg
     1801 tgccccatta aaccagttgc cgtgagagtt ggtgggcgtc gccaggctgt ggaatgtatc
     1861 gaggacttgc ttaacgagcc tgggcaacct ttggacttga gctgtaaacg ccccaggcca
     1921 taaggtgtaa acctgtgatt gcgtgtgtgg ttaacgcctt tgtttgctga atgagttgat
     1981 gtaagtttaa taaagggtga gataatgttt aacttgcatg gcgtgttaaa tggggcgggg
     2041 cttaaagggt atataatgcg ccgtgggcta atcttggtta catctgacct catggaggct
     2101 tgggagtgtt tggaagattt ttctgctgtg cgtaacttgc tggaacagag ctctaacagt
     2161 acctcttggt tttggaggtt tctgtggggc tcatcccagg caaagttagt ctgcagaatt
     2221 aaggaggatt acaagtggga atttgaagag cttttgaaat cctgtggtga gctgtttgat
     2281 tctttgaatc tgggtcacca ggcgcttttc caagagaagg tcatcaagac tttggatttt
     2341 tccacaccgg ggcgcgctgc ggctgctgtt gcttttttga gttttataaa ggataaatgg
     2401 agcgaagaaa cccatctgag cggggggtac ctgctggatt ttctggccat gcatctgtgg
     2461 agagcggttg tgagacacaa gaatcgcctg ctactgttgt cttccgtccg cccggcgata
     2521 ataccgacgg aggagcagca gcagcagcag gaggaagcca ggcggcggcg gcaggagcag
     2581 agcccatgga acccgagagc cggcctggac cctcgggaat gaatgttgta caggtggctg
     2641 aactgtatcc agaactgaga cgcattttga caattacaga ggatgggcag gggctaaagg
     2701 gggtaaagag ggagcggggg gcttgtgagg ctacagagga ggctaggaat ctagctttta
     2761 gcttaatgac cagacaccgt cctgagtgta ttacttttca acagatcaag gataattgcg
     2821 ctaatgagct tgatctgctg gcgcagaagt attccataga gcagctgacc acttactggc
     2881 tgcagccagg ggatgatttt gaggaggcta ttagggtata tgcaaaggtg gcacttaggc
     2941 cagattgcaa gtacaagatc agcaaacttg taaatatcag gaattgttgc tacatttctg
     3001 ggaacggggc cgaggtggag atagatacgg aggatagggt ggcctttaga tgtagcatga
     3061 taaatatgtg gccgggggtg cttggcatgg acggggtggt tattatgaat gtaaggttta
     3121 ctggccccaa ttttagcggt acggttttcc tggccaatac caaccttatc ctacacggtg
     3181 taagcttcta tgggtttaac aatacctgtg tggaagcctg gaccgatgta agggttcggg
     3241 gctgtgcctt ttactgctgc tggaaggggg tggtgtgtcg ccccaaaagc agggcttcaa
     3301 ttaagaaatg cctctttgaa aggtgtacct tgggtatcct gtctgagggt aactccaggg
     3361 tgcgccacaa tgtggcctcc gactgtggtt gcttcatgct agtgaaaagc gtggctgtga
     3421 ttaagcataa catggtatgt ggcaactgcg aggacagggc ctctcagatg ctgacctgct
     3481 cggacggcaa ctgtcacctg ctgaagacca ttcacgtagc cagccactct cgcaaggcct
     3541 ggccagtgtt tgagcataac atactgaccc gctgttcctt gcatttgggt aacaggaggg
     3601 gggtgttcct accttaccaa tgcaatttga gtcacactaa gatattgctt gagcccgaga
     3661 gcatgtccaa ggtgaacctg aacggggtgt ttgacatgac catgaagatc tggaaggtgc
     3721 tgaggtacga tgagacccgc accaggtgca gaccctgcga gtgtggcggt aaacatatta
     3781 ggaaccagcc tgtgatgctg gatgtgaccg aggagctgag gcccgatcac ttggtgctgg
     3841 cctgcacccg cgctgagttt ggctctagcg atgaagatac agattgaggt actgaaatgt
     3901 gtgggcgtgg cttaagggtg ggaaagaata tataaggtgg gggtcttatg tagttttgta
     3961 tctgttttgc agcagccgcc gccgccatga gcaccaactc gtttgatgga agcattgtga
     4021 gctcatattt gacaacgcgc atgcccccat gggccggggt gcgtcagaat gtgatgggct
     4081 ccagcattga tggtcgcccc gtcctgcccg caaactctac taccttgacc tacgagattc
     4141 gaacatcttt ctagaagatc tcctacaata ttctcagctg ccatggaaaa tcgatgttct
     4201 tcttttattc tctcaagatt ttcaggctgt atattaaaac ttatattaag aactatgcta
     4261 accacctcat caggaaccgt tgtaggtggc gtgggttttc ttggcaatcg actctcatga
     4321 aaactacgag ctaaatattc aatatgttcc tcttgaccaa ctttattctg catttttttt
     4381 gaacgaggtt tagagcaagc ttcaggaaac tgagacagga attttattaa aaatttaaat
     4441 tttgaagaaa gttcagggtt aatagcatcc attttttgct ttgcaagttc ctcagcattc
     4501 ttaacaaaag acgtctcttt tgacatgttt aaagtttaaa cctcctgtgt gaaattatta
     4561 tccgctcata attccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
     4621 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgccaattg ctttccagtc
     4681 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4741 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4801 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4861 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4921 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4981 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5041 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5101 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5161 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5221 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5281 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5341 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     5401 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5461 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5521 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5581 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5641 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5701 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5761 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5821 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5881 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5941 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6001 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6061 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6121 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6181 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6241 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6301 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6361 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6421 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6481 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6541 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6601 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6661 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6721 ctataaaaat aggcgtatca cgaggcc
//
