LOCUS       sequence-318562-       11623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(RBSmut)_Psyn-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..11623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  80..847
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     promoter        1927..1961
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        1983..2058
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(3501..3515,3516..3521,3522..3538,3539..3545)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    3521..3537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3553..3569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(3606..3632,3633..3635,3636..6122,6123..6125,
                     6126..7712)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7882..8673
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8796..9384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9285..9304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatttaaatt gaataaaatt gtaagaaaaa ttgaaagaat ttttttacat aaaaagttac
       61 atttattgaa gtaatttctg gtgatgctgc caacttactg atttagtgta tgatggtgtt
      121 tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg
      181 gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc tctcactgcc
      241 gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg caccagaaaa
      301 tcattgatat ggccatgaat ggcgttggat gccgggcaac agcccgcatt atgggcgttg
      361 gcctcaacac gattttacgt cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat
      421 acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg
      481 ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac agtctccgga agacggttgt
      541 tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga gcctgctgtc
      601 accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat cccgcctgaa
      661 gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc ataacctgaa
      721 tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga
      781 gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcaat aagttggagt
      841 cattaccgta atttcttact tgatactttt ctatttcatt aatctttctt tcttctttta
      901 tattattttt attatttaaa cactccttta aatctacact cccattttta gtaaatctat
      961 ctttaaggcc ccttgaaact ctattttgaa atcttaaatc ttttttttct tgaaagtatt
     1021 tatagatttt ttggtaacat tctttttttg aaaaattaag ttgatagtat atttcggtcc
     1081 ccaaatttac ccctaagtgt ttgtgataat tagttgtgac tttaaattct ttttctaatt
     1141 tgtaaaggta attttgcagt gtttttagtt taacgggtgt ttgactattt cttttaaggt
     1201 tttcattaaa gtaatagagt atgttttttt gtgtgtattt cttatatgtt ttatttatgt
     1261 acgctagtgt tgagataagg actactaatt tatactgtaa tttattgtag caagttgtgg
     1321 ttttactggt tttgtttata ttagataagt tttccattta tagctccttg aataccggat
     1381 tttttatcta taaagtatat atatcacaat tttgatttaa aagtaaattc tttttttaaa
     1441 aatacaaaaa ttttttataa attatataga ccatgatatt gtattaatac taattgattt
     1501 taatttaagg ttgaactaaa ataaacatag ttttgtagaa aataattttt cattattttg
     1561 ctacttgata ttggatcgta aaaatattag ggctttacaa agttctttta aaagagaatt
     1621 tagtaaagcc ctaagtcttt taataaaaaa ttttttgtaa aaaagttggc aaaaatagtt
     1681 tttgctatat atttatatat aagaaaaaat tcaatactca aggagataaa aaatggaaaa
     1741 cttaaacaat aatccacaaa aaattaatca aataaataag caaactaatt ttattggttt
     1801 tgaaaatttt atagaacttg ataatcaagt taatcaaact aaagaaaaaa gtaaattaag
     1861 taagataggt aagaaattac ccggaataag cagtcaagtc tagtgcggcc ggaattctaa
     1921 agatctttga cagctagctc agtcctaggt ataatactag tgaaaattta aatttctgac
     1981 ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     2041 gtggcaccga gtcggtgctt tttttggcgc gccaaataat gcccgtgtcc ctgttgaata
     2101 gggacacggt tttttaacaa tttttttatt cctaggcctt agtggccggt acccggggat
     2161 ccttattact ggccgctttc tagcctggat acttgtctgg ctagttgcat tagagagtca
     2221 gctagagccc tagggctagc tgtttgtgta ttcggagcca gtgtagtttt tctttttact
     2281 aacgatacgg gtaataattg attgcctttt acagcctgac cttgagacaa ttgcaaaagt
     2341 ctgtccacag aggtttgacc taataatcta aaatcttgtt tgatagtagt aagtggaggt
     2401 atgtagcaac tagaatcttc tgtatcatca taacccacaa ctgaaatatc agccccaact
     2461 ctaagtccac tttcagtaat agctcgcata gctcctaaag ccatttgatc attagcaact
     2521 aacatagcag tagggacaat gccttcattt aacatttgca ttgtttgctg aaatcctgac
     2581 atagcagacc aatctccttc tctttctgct attggttgta tttgatttct tgttaaatac
     2641 ttgtgccatc ctgctaatct caatctggca cttactgaac ttaagggacc tgcaagtagt
     2701 gcaatttgtt gatggcctaa tgcaacaagg tgttcaactc ctaaccttgt tccatcttcg
     2761 tgagaaaaaa ttatagagtt tattggggtt tggtccgaca catccaaaaa caatgctgga
     2821 acattagtac atgctgcttc aactgcaatc gcatcttggt catcaagagg ataattaatt
     2881 ataagtccac taacacgttg agctaataaa ttatgtactg ctgccttaca agcctctaca
     2941 cctgatctct caaccattga tacaacaacg cttgcaccaa gttgatcagc tctcgattta
     3001 attgcggcta caatttgact aggcgcatgt aatgcaagtg atgatgtggc aactccaatt
     3061 aaaagtgatt gtttccccgc aagttgctga gcaacgcgat ttggtatata attcaactct
     3121 gccatagctg cttcaacctt ctctcttgtt tttgcagata catgacttgc ttggttcaca
     3181 actctcgaaa ccgtttggta gctaaccccg gcatattcag caacatcata caaagtaaca
     3241 ggtttcatgg atcattcctc catgataaaa tttaaatttc tgactttggc aaatcctttt
     3301 gcctgttaaa aagaatagca caaaaattaa aaaaaaagaa acgcaaatag caatagcatt
     3361 aaaaataatt attattattt atttctttga ttaaaatcaa aagcctttaa tgtattttct
     3421 tgatctttca tgcgcttaac ggtaaaatcc aaggtattaa gttcaccttc cacttttctc
     3481 tctctatctt ctctagaaaa tcataaaaaa tttatttgct ttgtgagcgg ataacaatta
     3541 taatagattc aattgtgagc ggataacaat ttcacacaga attcattaaa gaagagaaat
     3601 tacatatgga taagaaatac tcaataggct tagctatcgg cacaaatagc gtcggatggg
     3661 cggtgatcac tgatgaatat aaggttccgt ctaaaaagtt caaggttctg ggaaatacag
     3721 accgccacag tatcaaaaaa aatcttatag gggctctttt atttgacagt ggagagacag
     3781 cggaagcgac tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg aagaatcgta
     3841 tttgttatct acaggagatt ttttcaaatg agatggcgaa agtagatgat agtttctttc
     3901 atcgacttga agagtctttt ttggtggaag aagacaagaa gcatgaacgt catcctattt
     3961 ttggaaatat agtagatgaa gttgcttatc atgagaaata tccaactatc tatcatctgc
     4021 gaaaaaaatt ggtagattct actgataaag cggatttgcg cttaatctat ttggccttag
     4081 cgcatatgat taagtttcgt ggtcattttt tgattgaggg agatttaaat cctgataata
     4141 gtgatgtgga caaactattt atccagttgg tacaaaccta caatcaatta tttgaagaaa
     4201 accctattaa cgcaagtgga gtagatgcta aagcgattct ttctgcacga ttgagtaaat
     4261 caagacgatt agaaaatctc attgctcagc tccccggtga gaagaaaaat ggcttatttg
     4321 ggaatctcat tgctttgtca ttgggtttga cccctaattt taaatcaaat tttgatttgg
     4381 cagaagatgc taaattacag ctttcaaaag atacttacga tgatgattta gataatttat
     4441 tggcgcaaat tggagatcaa tatgctgatt tgtttttggc agctaagaat ttatcagatg
     4501 ctattttact ttcagatatc ctaagagtaa atactgaaat aactaaggct cccctatcag
     4561 cttcaatgat taaacgctac gatgaacatc atcaagactt gactctttta aaagctttag
     4621 ttcgacaaca acttccagaa aagtataaag aaatcttttt tgatcaatca aaaaacggat
     4681 atgcaggtta tattgatggg ggagctagcc aagaagaatt ttataaattt atcaaaccaa
     4741 ttttagaaaa aatggatggt actgaggaat tattggtgaa actaaatcgt gaagatttgc
     4801 tgcgcaagca acggaccttt gacaacggct ctattcccca tcaaattcac ttgggtgagc
     4861 tgcatgctat tttgagaaga caagaagact tttatccatt tttaaaagac aatcgtgaga
     4921 agattgaaaa aatcttgact tttcgaattc cttattatgt tggtccattg gcgcgtggca
     4981 atagtcgttt tgcatggatg actcggaagt ctgaagaaac aattacccca tggaattttg
     5041 aagaagttgt cgataaaggt gcttcagctc aatcatttat tgaacgcatg acaaactttg
     5101 ataaaaatct tccaaatgaa aaagtactac caaaacatag tttgctttat gagtatttta
     5161 cggtttataa cgaattgaca aaggtcaaat atgttactga aggaatgcga aaaccagcat
     5221 ttctttcagg tgaacagaag aaagccattg ttgatttact cttcaaaaca aatcgaaaag
     5281 taaccgttaa gcaattaaaa gaagattatt tcaaaaaaat agaatgtttt gatagtgttg
     5341 aaatttcagg agttgaagat agatttaatg cttcattagg tacctaccat gatttgctaa
     5401 aaattattaa agataaagat tttttggata atgaagaaaa tgaagatatc ttagaggata
     5461 ttgttttaac attgacctta tttgaagata gggagatgat tgaggaaaga cttaaaacat
     5521 atgctcacct ctttgatgat aaggtgatga aacagcttaa acgtcgccgt tatactggtt
     5581 ggggacgttt gtctcgaaaa ttgattaatg gtattaggga taagcaatct ggcaaaacaa
     5641 tattagattt tttgaaatca gatggttttg ccaatcgcaa ttttatgcag ctgatccatg
     5701 atgatagttt gacatttaaa gaagacattc aaaaagcaca agtgtctgga caaggcgata
     5761 gtttacatga acatattgca aatttagctg gtagccctgc tattaaaaaa ggtattttac
     5821 agactgtaaa agttgttgat gaattggtca aagtaatggg gcggcataag ccagaaaata
     5881 tcgttattga aatggcacgt gaaaatcaga caactcaaaa gggccagaaa aattcgcgag
     5941 agcgtatgaa acgaatcgaa gaaggtatca aagaattagg aagtcagatt cttaaagagc
     6001 atcctgttga aaatactcaa ttgcaaaatg aaaagctcta tctctattat ctccaaaatg
     6061 gaagagacat gtatgtggac caagaattag atattaatcg tttaagtgat tatgatgtcg
     6121 atgccattgt tccacaaagt ttccttaaag acgattcaat agacaataag gtcttaacgc
     6181 gttctgataa aaatcgtggt aaatcggata acgttccaag tgaagaagta gtcaaaaaga
     6241 tgaaaaacta ttggagacaa cttctaaacg ccaagttaat cactcaacgt aagtttgata
     6301 atttaacgaa agctgaacgt ggaggtttga gtgaacttga taaagctggt tttatcaaac
     6361 gccaattggt tgaaactcgc caaatcacta agcatgtggc acaaattttg gatagtcgca
     6421 tgaatactaa atacgatgaa aatgataaac ttattcgaga ggttaaagtg attaccttaa
     6481 aatctaaatt agtttctgac ttccgaaaag atttccaatt ctataaagta cgtgagatta
     6541 acaattacca tcatgcccat gatgcgtatc taaatgccgt cgttggaact gctttgatta
     6601 agaaatatcc aaaacttgaa tcggagtttg tctatggtga ttataaagtt tatgatgttc
     6661 gtaaaatgat tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt
     6721 actctaatat catgaacttc ttcaaaacag aaattacact tgcaaatgga gagattcgca
     6781 aacgccctct aatcgaaact aatggggaaa ctggagaaat tgtctgggat aaagggcgag
     6841 attttgccac agtgcgcaaa gtattgtcca tgccccaagt caatattgtc aagaaaacag
     6901 aagtccagac aggcggattc tccaaggagt caattttacc aaaaagaaat tcggacaagc
     6961 ttattgctcg taaaaaagac tgggatccaa aaaaatatgg tggttttgat agtccaacgg
     7021 tagcttattc agtcctagtg gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat
     7081 ccgttaaaga gttactaggg atcacaattc tggaaagaag ttcctttgaa aaaaatccga
     7141 ttgacttttt agaagctaaa ggatataagg aagttaaaaa agacttaatc attaaactac
     7201 ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg gatgctggct agtgccggag
     7261 aattacaaaa aggaaatgag ctggctctgc caagcaaata tgtgaatttt ttatatttag
     7321 ctagtcatta tgaaaagttg aagggtagtc cagaagataa cgaacaaaaa caattgtttg
     7381 tggagcagca taagcattat ttagatgaga ttattgagca aatcagtgaa ttttctaagc
     7441 gtgttatttt agcagatgcc aatttagata aagttcttag tgcatataac aaacatagag
     7501 acaaaccaat acgtgaacaa gcagaaaata ttattcattt atttacgttg acgaatcttg
     7561 gagctcccgc tgcttttaaa tattttgata caacaattga tcgtaaacga tatacgtcta
     7621 caaaagaagt tttagatgcc actcttatcc atcaatccat cactggtctt tatgaaacac
     7681 gcattgattt gagtcagcta ggaggtgact aataaaagct tgatcagatc tcagcttttt
     7741 tttgaagtgc ctggcagtaa gttgaaaaaa tttgaaataa acttggacta tgttaataaa
     7801 aaaatacaaa ttaacgaaca aaaagatttg aattattata taatttagtt aaaagcaatt
     7861 ttaaatgagg gaggtttcca tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     7921 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     7981 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8041 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8101 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8161 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8221 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8281 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8341 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8401 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8461 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8521 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     8581 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     8641 aaaggcgaga tcaccaaggt agtcggcaaa taatgacgtc gccggagcgg tcgagttctg
     8701 gaccgaccgg ctcgggttct catgaccaaa atcccttaac gtgagttttc gttccactga
     8761 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     8821 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     8881 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     8941 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9001 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9061 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9121 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9181 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9241 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9301 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9361 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9421 ttttgctggc cttttgctca catgttcttt cctgcactag atccccggca agttcatcct
     9481 tagggttttc atatctctta aaaagtcaat ttctgcttga ttaatttctt gtggattatt
     9541 gtttttgcgg tttttcattt tttatctcct tgagtattga attttttctt atatataaat
     9601 atatagcaaa aactattttt gccaactttt ttacaaaaaa ttttttatta aaacacttag
     9661 ggctttgcta aattctcttt taaaagaact tagtaaagcc ctatggattt aagaactgat
     9721 tatacttacg tagtaaaaat actacagata ttaatagtat agaccatatc aaaatcataa
     9781 tcaatttata tttatctaaa attgtagtcc atacaaaata caaaaaaatt aggtttttct
     9841 aaattctatt ttaaaagaac ttagtaacac ctaatctcta aattacttac aaaattctaa
     9901 aattagattt ttagttcttc atatttcttt aaaagttttt taagaaaatc tttttgattt
     9961 tcaaaaattt catgcatcat aaaactagta aatttggatt tttttttgta aaaatcataa
    10021 ctttcttggg ttttaagttg aaatcttaat ggttttattg gattttgcct agattttttt
    10081 aacgccggac tttctttatc ttttataaaa ttataagaat ttttaatacc attttctatt
    10141 aaataatctt cttttataag cccctcttgc agtgcagttg ctaatcttaa atacttatac
    10201 gcttgagttt ttgctacatc aaagtctttt ataaaatcaa gaaaagtttt ataaccatca
    10261 aatttataat attgattatc ttttatttct tttaaaattt taatattttc caccctgtgg
    10321 aatatttctt ttttgaaatt gtcatttaac ttttccttta gtgtcttgta ataagaaatt
    10381 ttactttcat taatcattaa attttgttca acataagtat ctatcttttt tgttaaaact
    10441 ttaacaacac gttttttttc cataaaaatt ttcctttatt tttgcaacat tttccttaat
    10501 tgatataatt gttactattt tagatttttt aatatccact taagaattaa tccctcacta
    10561 gaatattttt tattatagaa ttcatataca tgtttttcta aatctataaa tttatcaaca
    10621 attgctttat tatagggtgt atctatcttt tcttttctta ataatcgtct catattcttt
    10681 aaataacaac aaacactgcc ggttttaaac ctaaattcta tgtaatatat ttttgaaaaa
    10741 gtatatacat tttcaacccc gtttatttca tactttacaa aaacattatt tattggtttt
    10801 ctataaccat agtaaatgcc taaaaatttg tccccgtctt tcataggata tagataaagt
    10861 ttttgaatct ctgatttatc taataatttt tcaaaaaaaa ttatacaccg cttttgtttg
    10921 ttactaacta ctctatgcaa tgcctttaat atttttgtgt gatatatatt tttatcacct
    10981 tttttttctt tcagcaggaa gatactttct tttggaaatt taattttttg ttcatttatt
    11041 cttgcagcct taagtccgat tttcatattt aatccttata tagcctttct atttaattct
    11101 tttaaactca ataaactttt ttggtttttc attagttcta ataattcgca ataatatgtg
    11161 caaaacactt tattgtattc tattttcttt tggttattca aatattcttt tataattggc
    11221 tttagaagtt caatatttac ttcttttttt agttgttcaa taaggatatt gaaaatgttt
    11281 gtttttaaat cttgataatt ttgttgagaa ttttcttttt ttctttcaat tgacttctct
    11341 aatttacgct ttatattgtt taaatcgcta tatttatgac tttcaataat aaaatgaggc
    11401 ttaaatttgt aattttcgta tattttttgg aaacttgttt ctaattgttt agaattatat
    11461 tcattttttt caaattcttt ttgagtgttg catagaattt cctttaattt ttcttgtttt
    11521 tctttcatac aagatttagt aaaatgtata tttttatttt ttagtaattt aatttctatt
    11581 atttttatga ttttaagtat tttgattata ttatctttat caa
//
