LOCUS       sequence-318567-        6627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Block 3 for AdenoBuilder genome assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Miciak JJ, Hirshberg J, Bunz F
  TITLE     Seamless assembly of recombinant adenoviral genomes from high-copy 
            plasmids.
  JOURNAL   PLoS One. 2018 Jun 27;13(6):e0199563. doi: 
            10.1371/journal.pone.0199563. eCollection 2018.
  PUBMED    29949649
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-06-27"; volume: "13(6):e0199563. doi"; pages: " 
            10.1371/journal.pone.0199563. eCollection 2018"
COMMENT     Sequence Label: pAd5-B3
FEATURES             Location/Qualifiers
     source          1..6627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3761..3772)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(join(4459..4465,4466..4483,4484..4489))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    4504..4525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4645..4662)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4816..5404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4896..4915)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5575..6366,6367..6435))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6198..6217
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6436..6540)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6608..6626
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcttcgaacg aaagcattaa gtggctcgct ccctgtagcc ggagggttat
      421 tttccaaggg ttgagtcgcg ggacccccgg ttcgagtctc ggaccggccg gactgcggcg
      481 aacgggggtt tgcctccccg tcatgcaaga ccccgcttgc aaattcctcc ggaaacaggg
      541 acgagcccct tttttgcttt tcccagatgc atccggtgct gcggcagatg cgcccccctc
      601 ctcagcagcg gcaagagcaa gagcagcggc agacatgcag ggcaccctcc cctcctccta
      661 ccgcgtcagg aggggcgaca tccgcggttg acgcggcagc agatggtgat tacgaacccc
      721 cgcggcgccg ggcccggcac tacctggact tggaggaggg cgagggcctg gcgcggctag
      781 gagcgccctc tcctgagcgg cacccaaggg tgcagctgaa gcgtgatacg cgtgaggcgt
      841 acgtgccgcg gcagaacctg tttcgcgacc gcgagggaga ggagcccgag gagatgcggg
      901 atcgaaagtt ccacgcaggg cgcgagctgc ggcatggcct gaatcgcgag cggttgctgc
      961 gcgaggagga ctttgagccc gacgcgcgaa ccgggattag tcccgcgcgc gcacacgtgg
     1021 cggccgccga cctggtaacc gcatacgagc agacggtgaa ccaggagatt aactttcaaa
     1081 aaagctttaa caaccacgtg cgtacgcttg tggcgcgcga ggaggtggct ataggactga
     1141 tgcatctgtg ggactttgta agcgcgctgg agcaaaaccc aaatagcaag ccgctcatgg
     1201 cgcagctgtt ccttatagtg cagcacagca gggacaacga ggcattcagg gatgcgctgc
     1261 taaacatagt agagcccgag ggccgctggc tgctcgattt gataaacatc ctgcagagca
     1321 tagtggtgca ggagcgcagc ttgagcctgg ctgacaaggt ggccgccatc aactattcca
     1381 tgcttagcct gggcaagttt tacgcccgca agatatacca taccccttac gttcccatag
     1441 acaaggaggt aaagatcgag gggttctaca tgcgcatggc gctgaaggtg cttaccttga
     1501 gcgacgacct gggcgtttat cgcaacgagc gcatccacaa ggccgtgagc gtgagccggc
     1561 ggcgcgagct cagcgaccgc gagctgatgc acagcctgca aagggccctg gctggcacgg
     1621 gcagcggcga tagagaggcc gagtcctact ttgacgcggg cgctgacctg cgctgggccc
     1681 caagccgacg cgccctggag gcagctgggg ccggacctgg gctggcggtg gcacccgcgc
     1741 gcgctggcaa cgtcggcggc gtggaggaat atgacgagga cgatgagtac gagccagagg
     1801 acggcgagta ctaagcggtg atgtttctga tcagatgatg caagacgcaa cggacccggc
     1861 ggtgcgggcg gcgctgcaga gccagccgtc cggccttaac tccacggacg actggcgcca
     1921 ggtcatggac cgcatcatgt cgctgactgc gcgcaatcct gacgcgttcc ggcagcagcc
     1981 gcaggccaac cggctctccg caattctgga agcggtggtc ccggcgcgcg caaaccccac
     2041 gcacgagaag gtgctggcga tcgtaaacgc gctggccgaa aacagggcca tccggcccga
     2101 cgaggccggc ctggtctacg acgcgctgct tcagcgcgtg gctcgttaca acagcggcaa
     2161 cgtgcagacc aacctggacc ggctggtggg ggatgtgcgc gaggccgtgg cgcagcgtga
     2221 gcgcgcgcag cagcagggca acctgggctc catggttgca ctaaacgcct tcctgagtac
     2281 acagcccgcc aacgtgccgc ggggacagga ggactacacc aactttgtga gcgcactgcg
     2341 gctaatggtg actgagacac cgcaaagtga ggtgtaccag tctgggccag actatttttt
     2401 ccagaccagt agacaaggcc tgcagaccgt aaacctgagc caggctttca aaaacttgca
     2461 ggggctgtgg ggggtgcggg ctcccacagg cgaccgcgcg accgtgtcta gcttgctgac
     2521 gcccaactcg cgcctgttgc tgctgctaat agcgcccttc acggacagtg gcagcgtgtc
     2581 ccgggacaca tacctaggtc acttgctgac actgtaccgc gaggccatag gtcaggcgca
     2641 tgtggacgag catactttcc aggagattac aagtgtcagc cgcgcgctgg ggcaggagga
     2701 cacgggcagc ctggaggcaa ccctaaacta cctgctgacc aaccggcggc agaagatccc
     2761 ctcgttgcac agtttaaaca gcgaggagga gcgcattttg cgctacgtgc agcagagcgt
     2821 gagccttaac ctgatgcgcg acggggtaac gcccagcgtg gcgctggaca tgaccgcgcg
     2881 caacatggaa ccgggcatgt atgcctcaaa ccggccgttt atcaaccgcc taatggacta
     2941 cttgcatcgc gcggccgccg tgaaccccga gtatttcacc aatgccatct tgaacccgca
     3001 ctggctaccg ccccctggtt tctacaccgg gggattcgag gtgcccgagg gtaacgatgg
     3061 attcctctgg gacgacatag acgacagcgt gttttccccg caaccgcaga ccctgctaga
     3121 gttgcaacag cgcgagcagg cagaggcggc gctgcgaaag gaaagcttcc gcaggccaag
     3181 cagcttgtcc gatctaggcg ctgcggcccc gcggtcagat gctagtagcc catttccaag
     3241 cttgataggg tctcttacca gcactcgcac cacccgcccg cgcctgctgg gcgaggagga
     3301 gtacctaaac aactcgctgc tgcagccgca gcgcgaaaaa aacctgcctc cggcatttcc
     3361 caacaacggg atagagagcc tagtggacaa gatgagtaga tggaagacgt acgcgcagga
     3421 gcacagggac gtgccaggcc cgcgcccgcc cacccgtcgt caaaggcacg accgtcagcg
     3481 gggtctggtg tgggaggacg atgactcggc agacgacagc agcgtcctgg atttgggagg
     3541 gagtggcaac ccgtttgcgc accttcgccc caggctgggg agaatgtttt aaaaaaaaaa
     3601 aaagcatgat gcaaaataaa aaactcacca aggccatggc accgagcgtt ggttttcttg
     3661 tattcccctt agtatgcggc gcgcggcgat gtatgaggaa ggtcctcctc cctcctacga
     3721 gagtgtggtg agcgcggcgc cagtggcggc ggcgctgggt tctcccttcg atgctcccct
     3781 ggacccgccg tttgtgcctc cgcggtacct gcggcctacc ggggggagaa acagcatccg
     3841 ttactctgag ttggcacccc tattcgacac cacccgtgtg tacctggtgg acaacaagtc
     3901 aacggatgtg gcatccctga actaccagaa cgaccacagc aactttctga ccacggtcat
     3961 tcaaaacaat gactacagcc cgggggaggc aagcacacag accatcaatc ttgacgattc
     4021 gaacatcttt ctagaagatc tcctacaata ttctcagctg ccatggaaaa tcgatgttct
     4081 tcttttattc tctcaagatt ttcaggctgt atattaaaac ttatattaag aactatgcta
     4141 accacctcat caggaaccgt tgtaggtggc gtgggttttc ttggcaatcg actctcatga
     4201 aaactacgag ctaaatattc aatatgttcc tcttgaccaa ctttattctg catttttttt
     4261 gaacgaggtt tagagcaagc ttcaggaaac tgagacagga attttattaa aaatttaaat
     4321 tttgaagaaa gttcagggtt aatagcatcc attttttgct ttgcaagttc ctcagcattc
     4381 ttaacaaaag acgtctcttt tgacatgttt aaagtttaaa cctcctgtgt gaaattatta
     4441 tccgctcata attccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
     4501 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgccaattg ctttccagtc
     4561 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4621 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4681 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4741 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4801 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4861 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4921 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4981 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5041 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5101 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5161 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5221 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     5281 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5341 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5401 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5461 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5521 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5581 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5641 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5701 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5761 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5821 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5881 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5941 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6001 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6061 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6121 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6181 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6241 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6301 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6361 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6421 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6481 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6541 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6601 ctataaaaat aggcgtatca cgaggcc
//
