LOCUS       sequence-318578-        3742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified block 1, with deletion in E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Miciak JJ, Hirshberg J, Bunz F
  TITLE     Seamless assembly of recombinant adenoviral genomes from high-copy 
            plasmids.
  JOURNAL   PLoS One. 2018 Jun 27;13(6):e0199563. doi: 
            10.1371/journal.pone.0199563. eCollection 2018.
  PUBMED    29949649
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-06-27"; volume: "13(6):e0199563. doi"; pages: " 
            10.1371/journal.pone.0199563. eCollection 2018"
COMMENT     Sequence Label: pAd5-B1-deltaE1-MCS
FEATURES             Location/Qualifiers
     source          1..3742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   379..481
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     569..719
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        complement(join(1574..1580,1581..1598,1599..1604))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1619..1640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1760..1777)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1931..2519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2011..2030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2690..3481,3482..3550))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3313..3332
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3551..3655)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3723..3741
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcttcgaaca tcatcaataa tataccttat tttggattga agccaatatg
      421 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
      481 gtagtgtggc ggaagtgtga tgttgcaagt gtggcggaac acatgtaagc gacggatgtg
      541 gcaaaagtga cgtttttggt gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt
      601 tttaggcgga tgttgtagta aatttgggcg taaccgagta agatttggcc attttcgcgg
      661 gaaaactgaa taagaggaag tgaaatctga ataattttgt gttactcata gcgcgtaata
      721 tttgtctagg gccgcgggga ctttgaccgt ttacgtggag actcgcccag gtgtttttct
      781 caggtgtttt ccgcgttccg ggtcaaagtt ggcgttttat tattatagtc agctgacgtg
      841 tagtgtattt atacccggga attcggatcc accggtgata tcttaattaa gtcgacacta
      901 gttggcttaa gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt
      961 tttgcagcag ccgccgccgc catgagcacc aactcgtttg atggaagcat tgtgagctca
     1021 tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat gggctccagc
     1081 attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga gattcgaaca
     1141 tctttctaga agatctccta caatattctc agctgccatg gaaaatcgat gttcttcttt
     1201 tattctctca agattttcag gctgtatatt aaaacttata ttaagaacta tgctaaccac
     1261 ctcatcagga accgttgtag gtggcgtggg ttttcttggc aatcgactct catgaaaact
     1321 acgagctaaa tattcaatat gttcctcttg accaacttta ttctgcattt tttttgaacg
     1381 aggtttagag caagcttcag gaaactgaga caggaatttt attaaaaatt taaattttga
     1441 agaaagttca gggttaatag catccatttt ttgctttgca agttcctcag cattcttaac
     1501 aaaagacgtc tcttttgaca tgtttaaagt ttaaacctcc tgtgtgaaat tattatccgc
     1561 tcataattcc acacattata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1621 gagtgagcta actcacatta attgcgttgc gctcactgcc aattgctttc cagtcgggaa
     1681 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1741 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1801 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1861 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1921 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1981 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2041 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2101 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2161 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2221 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2281 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2341 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2401 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2461 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2521 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2581 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2641 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2701 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2761 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2821 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2881 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2941 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3001 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3061 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3121 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3181 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3241 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3301 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3361 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3421 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3481 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3541 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3601 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3661 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3721 aaaataggcg tatcacgagg cc
//
