LOCUS       sequence-318591-       10565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Myc-tagged mouse Lrrk2 without kinase domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10565)
  TITLE     Lrrk2 expression plasmids
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc
FEATURES             Location/Qualifiers
     source          1..10565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      665..674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             680..703
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             1842..1850
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6284..6313
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6342..6359)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6348..6572
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6705..6724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(6748..6768)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6753..7082
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6933..7068
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7175..7774
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7175..7194)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7671..7691
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8004..8125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8041..8060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8095..8114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8174..8190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8174..8190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8187..8209)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8198..8214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8222..8228,8229..8246,8247..8252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8267..8288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8405..8422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8576..9164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8656..8675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9335..10126,10127..10195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9958..9977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10196..10300)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10375..10394)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagccacc atggcaagct ggagccaccc gcagttcgaa aaagccggca tggccagtgg
      721 cgcctgtcag ggctgcgaag aggaagagga ggaggaggct ctgaagaagt tgatagtcag
      781 gctgaataat gtccaggaag gcaagcagat cgagacgttg cttcagctcc tggaggacat
      841 gctggtgttc acctactcgg accgcgcctc caagttattt gaagataaaa atttccacgt
      901 gcctctgttg attgtcctgg actcctacat gagagttgcc agtgtacagc aggcggggtg
      961 gtcacttctg tgcaaattaa tagaagtctg tccagggaca ttgcaaagct taataggacc
     1021 ccaggatatt ggaaatgatt gggaagtcct tggtattcac cggctgattc ttaaaatgtt
     1081 aactgttcat cacgccaatg taaacctgtc aatagttgga ctaaaagcct tggatctcct
     1141 cctagattca ggtaaactca ccttgctgat actggatgaa gaatgtgata ttttcttgtt
     1201 aatttttgat gccatgcaca gatattcagc caatgatgaa gtccaaaaac tgggatgcaa
     1261 agctttacac gtgctttttg agagagtttc cgaggaacag ctgactgagt ttgtggagaa
     1321 caaagattac acgatactgc tgagtacgtt cggcagcttc agaagggaca aggagattgt
     1381 gtaccacgta ctttgctgct tgcattccct ggcggttaca tgcagcaatg tagaggtcct
     1441 catgagtggg aatgtccggt gctacaatct tgtggtggag gccatgaaag ccttccccac
     1501 caatgaaaac atccaagagg tgagctgctc cttgttccag aagcttacat taggtaactt
     1561 tttcaacatc ctggtgttga acgaagtgca tgtctttgtg gtgaaagcgg tccgacagta
     1621 tcctgagaac gcagccttac agatctctgc actcagctgt ttagcactcc tcactgagac
     1681 tattttctta aaccaagact tggaggaaag aagtgagact caagagcaga gcgaagagga
     1741 agacagtgag aagcttttct ggctggaacc ctgctataaa gccctggtgc gccatcgaaa
     1801 ggacaaacac gtgcaggagg ctgcctgctg ggcactaaat aacctcctta tgtaccagaa
     1861 cagtttgcat gagaagatcg gagatgaaga tggccagttc cctgcgcaca gggaagtgat
     1921 gctgtctatg ctgatgcact cttcttccaa agatgtcttc caagcagctg cacatgctct
     1981 gtccactctc ttggaacaaa atgttaattt caggaaaatc ctgctggcaa aaggagtata
     2041 cctgaatgtc ttggaattga tgcagaagca tgcccatgcg cctgaggtgg cagagagtgg
     2101 ctgcaagatg ctgagtcacc tgtttgaagg aagtaaccct tctctggata caatggcagc
     2161 agtggtccct aaaatactaa cagtgatgaa agcccacgga acgtctctgt cagtccagct
     2221 ggaggcgctg cgagctatct tgcatttcgt tgtgccagga ctattggaag aatccaggga
     2281 ggactctcaa tgcagaccaa atgtgctcag aaaacagtgt ttcaggactg acatccacaa
     2341 gctggttcta gtcgctctga acaggttcat tgggaatcct gggattcaga aatgtggatt
     2401 gaaagtaatc tcttctctcg cgcaccttcc tgatgccaca gagacattgt ccctgcaagg
     2461 agcagttgac tcagtcctcc acaccttaca gatgtatcca gatgaccaag aaattcagtg
     2521 tctgggctta caccttatgg gatgcttgat gacaaagaag aatttctgca tagggacagg
     2581 gcacctcctg gcaaaaattc tggcttccac tttgcagcgc tttaaagatg ttgctgaggt
     2641 gcagactaca ggattacaga caaccctgtc aatacttgag ctgtcagtat ctttctccaa
     2701 gctgctagtg cactattcct ttgatgtggt gatatttcat cagatgtctt ccagtgttgt
     2761 agaacaaaag gatgagcagt tcctcaatct atgttgcaaa tgctttgcaa aagtggccgt
     2821 ggatgatgag ctgaaaaaca ccatgctaga gagagcctgc gatcagaata acagcatcat
     2881 ggttgaatgt ttgctcctct tgggagctga tgccaaccaa gtgaaggggg caacttcttt
     2941 aatctatcag gtatgtgaga aagagagcag tcctaaattg gtggaactgt tgcttaatgg
     3001 tggttgtcgt gaacaagatg tacggaaggc cctgaccgta agcatccaaa agggcgacag
     3061 ccaggtcatc agcttgctcc tcaggaaact tgccctggac ctggccaaca acagcatttg
     3121 ccttggagga tttggcatag gaaaaattga tccttcttgg cttggtcctt tatttccaga
     3181 taagtcatcc aatttaagga agcaaacaaa cacaggatct gtcctagcga ggaaagtgct
     3241 ccggtatcag atgagaaaca cccttcaaga aggcgtggcc tcaggcagtg acggcaagtt
     3301 ttctgaagac gcgctggcga aatttggaga atggaccttt attcccgact cttctatgga
     3361 cagtgtgttt ggccagagcg atgatctgga tagcgaaggc agcgagagct catttctcgt
     3421 gaagaggaag tccaactcaa ttagtgtagg ggaagtttac agagatctag ctctgcagcg
     3481 ctgctcacca aatgctcaga ggcattccaa ttcgctgggt cctgtttttg accatgaaga
     3541 cttactgaga cgaaaaagaa aaatactgtc ttcagatgag tctctcaggt cctcaaggct
     3601 gccgtcccat atgaggcaat cagatagctc ttcttccctg gcttctgaga gagaacacat
     3661 cacgtcgtta gacctatctg ccaacgaact caaagatatt gatgctctga gccagaagtg
     3721 ttgcctcagt agccacctgg aacatctcac caaactggaa cttcaccaga attcactcac
     3781 gagcttccca cagcagctgt gtgagactct gaagtgtttg atacacttgg atttgcacag
     3841 taacaaattc acctcatttc cctctttcgt gttgaaaatg ccacgtatca ccaacctaga
     3901 tgcctctcga aatgacatcg ggccaacagt agttttagac cctgcgatga agtgtccaag
     3961 cctcaaacag ttgaatctgt cctataacca gctctcttca atcccagaga atcttgccca
     4021 agtggtggag aaacttgagc agctcctact ggaaggaaat aaaatatccg ggatttgctc
     4081 tcccctgagc ctgaaggaac tgaagatttt aaatcttagt aaaaatcaca ttccatccct
     4141 acctggagat tttcttgagg cttgttcaaa agtcgagagt ttcagtgctc gcatgaattt
     4201 tcttgctgca atgcctgcct taccttcttc cataacgagc ttaaaattgt ctcagaactc
     4261 tttcacgtgc attccagaag cgattttcag tcttccgcac ttgcggtcct tggatatgag
     4321 ccacaacaac attgaatgtc tgccgggacc tgcacattgg aagtctctga acttaaggga
     4381 actcattttt agcaagaatc agatcagcac cttagacttt agtgagaacc cacacgtgtg
     4441 gtcaagagta gagaaactgc atctctctca taataaactg aaagagattc ctccagaaat
     4501 tggctgcctt gaaaatctga cgtctctcga cgtcagttac aacttggaac tgaggtcctt
     4561 cccaaatgaa atggggaagt tgagcaagat atgggatctt cccttggacg gactgcatct
     4621 gaattttgac tttaagcacg taggatgcaa ggccaaagac atcataaggt ttctacaaca
     4681 acgtctgaaa aaggctgtac cctacaaccg aatgaagctc atgattgtgg gaaatacggg
     4741 gagcggtaag accactttac tgcaacaact catgaaaatg aagaaaccag aacttggcat
     4801 gcagggtgcc acagtcggca tagacgtgcg agactggtcc atccaaatac ggggcaaaag
     4861 gagaaaggac ctggttctaa acgtgtggga ttttgcaggt cgtgaggaat tctacagcac
     4921 tcacccccac ttcatgaccc agagagccct ctacctggct gtctatgatc tcagcaaggg
     4981 gcaggcagag gtggacgcca tgaagccctg gctcttcaat atcaaggctc gtgcctcttc
     5041 ttccccggtg attctggtgg gcacacattt ggatgtttct gatgagaagc agcggaaagc
     5101 gtgcataagc aaaatcacga aggaactcct aaataagcga ggattcccca ccatccggga
     5161 ctaccacttt gtgaatgcca ccgaggagtc agatgcgctg gcaaagcttc ggaaaaccat
     5221 cataaatgag agccttaatt tcaagatccg agatcagcct gtggttgggc agctaattcc
     5281 agattgctac gtagaactgg agaaaatcat tttatcagag cggaaagctg tgccgactga
     5341 gtttcctgtg attaaccgga aacacctgtt acagctcgtg aacgaacatc agctgcagct
     5401 ggatgagaac gagctcccac acgccgttca cttcctaaat gagtcgggag ttcttctgca
     5461 ttttcaagac cctgccctgc agctaagtga cctgtacttt gtggaaccca agtggctttg
     5521 taaagtcatg gcacagatct tgacagtgaa ggtagacggc tgtctgaaac atcctaaggg
     5581 catcatttcc cggagagatg tggaaaaatt cctttcaaag aagaagcgat tcccgaagaa
     5641 ctatatgatg caatacttta aactattaga aaaatttcag atcgcattgc caatagggga
     5701 agaatatctt ctggttccaa gcagcttgtc tgaccacagg ccagtgatag agctccccca
     5761 ctgtgagaac tctgagatca tcatccggct gtacgaaatg ccgtactttc ccatgggatt
     5821 ttggtcaaga ttgattaacc gattacttga aatctcaccc ttcatgcttt ctggcagaga
     5881 gagagcacta cgccctaaca ggatgtattg gcggcaaggc atctacttga attggtctcc
     5941 agaagcatac tgtctggtag gctctgaagt cttagacaat cgacctgaga gtttcttgaa
     6001 aatcacagtt ccgtcttgta gaaaaggttg tattcttctg ggccgagttg tggatcatat
     6061 tgactcactc atggaagaat ggtttcccgg gttactggag attgacattt gtggggaagg
     6121 agaaactctg ttgaagaaat gggcattgta cagttttaat gatggtgaag aacatcagaa
     6181 gatcttgctt gatgagttga tgaagaaggc tgaagaagga gacctgttaa taaatccaga
     6241 ccaaccaagg ctcactattc caatatccca gattgctccg gtcgagcaaa aactcatctc
     6301 agaagaggat ctgtaggggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     6361 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6421 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6481 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6541 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6601 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6721 cccttccttt ctcgccacgt tcgctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6841 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6901 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6961 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7021 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     7081 aaaagcttgc atgcctgcag gtcggccgcc acgaccggtg ccgccaccat cccctgaccc
     7141 acgcccctga cccctcacaa ggagacgacc ttccatgacc gagtacaagc ccacggtgcg
     7201 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
     7261 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
     7321 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     7381 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7441 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7501 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7561 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7621 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7681 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7741 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     7801 gaccgaaagg agcgcacgac cccatggctc cgaccgaagc cacccggggc ggccccgccg
     7861 accccgcacc cgcccccgag gcccaccgac tctagaggat cataatcagc cataccacat
     7921 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     7981 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     8041 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8101 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     8161 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8221 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8281 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8341 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8401 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8461 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8521 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8581 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8641 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8701 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8761 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8821 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8881 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8941 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9001 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9061 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9121 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9181 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9241 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9301 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9361 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9421 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9481 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9541 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9601 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9661 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9721 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9781 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9841 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9901 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9961 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10021 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10081 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10141 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10201 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10261 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10321 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
    10381 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
    10441 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
    10501 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
    10561 gcgtt
//
