LOCUS       sequence-318592-       10019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Myc-tagged mouse Lrrk2 without GTPase domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10019)
  TITLE     Lrrk2 expression plasmids
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc
FEATURES             Location/Qualifiers
     source          1..10019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      665..674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             680..703
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             1842..1850
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5738..5767
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(5796..5813)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5802..6026
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6159..6178)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(6202..6222)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6207..6536
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6387..6522
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6629..7228
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6629..6648)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7125..7145
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7458..7579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7495..7514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7549..7568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7628..7644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7628..7644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7641..7663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7652..7668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7676..7682,7683..7700,7701..7706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7721..7742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7859..7876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8030..8618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8110..8129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8789..9580,9581..9649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9412..9431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9650..9754)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9829..9848)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagccacc atggcaagct ggagccaccc gcagttcgaa aaagccggca tggccagtgg
      721 cgcctgtcag ggctgcgaag aggaagagga ggaggaggct ctgaagaagt tgatagtcag
      781 gctgaataat gtccaggaag gcaagcagat cgagacgttg cttcagctcc tggaggacat
      841 gctggtgttc acctactcgg accgcgcctc caagttattt gaagataaaa atttccacgt
      901 gcctctgttg attgtcctgg actcctacat gagagttgcc agtgtacagc aggcggggtg
      961 gtcacttctg tgcaaattaa tagaagtctg tccagggaca ttgcaaagct taataggacc
     1021 ccaggatatt ggaaatgatt gggaagtcct tggtattcac cggctgattc ttaaaatgtt
     1081 aactgttcat cacgccaatg taaacctgtc aatagttgga ctaaaagcct tggatctcct
     1141 cctagattca ggtaaactca ccttgctgat actggatgaa gaatgtgata ttttcttgtt
     1201 aatttttgat gccatgcaca gatattcagc caatgatgaa gtccaaaaac tgggatgcaa
     1261 agctttacac gtgctttttg agagagtttc cgaggaacag ctgactgagt ttgtggagaa
     1321 caaagattac acgatactgc tgagtacgtt cggcagcttc agaagggaca aggagattgt
     1381 gtaccacgta ctttgctgct tgcattccct ggcggttaca tgcagcaatg tagaggtcct
     1441 catgagtggg aatgtccggt gctacaatct tgtggtggag gccatgaaag ccttccccac
     1501 caatgaaaac atccaagagg tgagctgctc cttgttccag aagcttacat taggtaactt
     1561 tttcaacatc ctggtgttga acgaagtgca tgtctttgtg gtgaaagcgg tccgacagta
     1621 tcctgagaac gcagccttac agatctctgc actcagctgt ttagcactcc tcactgagac
     1681 tattttctta aaccaagact tggaggaaag aagtgagact caagagcaga gcgaagagga
     1741 agacagtgag aagcttttct ggctggaacc ctgctataaa gccctggtgc gccatcgaaa
     1801 ggacaaacac gtgcaggagg ctgcctgctg ggcactaaat aacctcctta tgtaccagaa
     1861 cagtttgcat gagaagatcg gagatgaaga tggccagttc cctgcgcaca gggaagtgat
     1921 gctgtctatg ctgatgcact cttcttccaa agatgtcttc caagcagctg cacatgctct
     1981 gtccactctc ttggaacaaa atgttaattt caggaaaatc ctgctggcaa aaggagtata
     2041 cctgaatgtc ttggaattga tgcagaagca tgcccatgcg cctgaggtgg cagagagtgg
     2101 ctgcaagatg ctgagtcacc tgtttgaagg aagtaaccct tctctggata caatggcagc
     2161 agtggtccct aaaatactaa cagtgatgaa agcccacgga acgtctctgt cagtccagct
     2221 ggaggcgctg cgagctatct tgcatttcgt tgtgccagga ctattggaag aatccaggga
     2281 ggactctcaa tgcagaccaa atgtgctcag aaaacagtgt ttcaggactg acatccacaa
     2341 gctggttcta gtcgctctga acaggttcat tgggaatcct gggattcaga aatgtggatt
     2401 gaaagtaatc tcttctctcg cgcaccttcc tgatgccaca gagacattgt ccctgcaagg
     2461 agcagttgac tcagtcctcc acaccttaca gatgtatcca gatgaccaag aaattcagtg
     2521 tctgggctta caccttatgg gatgcttgat gacaaagaag aatttctgca tagggacagg
     2581 gcacctcctg gcaaaaattc tggcttccac tttgcagcgc tttaaagatg ttgctgaggt
     2641 gcagactaca ggattacaga caaccctgtc aatacttgag ctgtcagtat ctttctccaa
     2701 gctgctagtg cactattcct ttgatgtggt gatatttcat cagatgtctt ccagtgttgt
     2761 agaacaaaag gatgagcagt tcctcaatct atgttgcaaa tgctttgcaa aagtggccgt
     2821 ggatgatgag ctgaaaaaca ccatgctaga gagagcctgc gatcagaata acagcatcat
     2881 ggttgaatgt ttgctcctct tgggagctga tgccaaccaa gtgaaggggg caacttcttt
     2941 aatctatcag gtatgtgaga aagagagcag tcctaaattg gtggaactgt tgcttaatgg
     3001 tggttgtcgt gaacaagatg tacggaaggc cctgaccgta agcatccaaa agggcgacag
     3061 ccaggtcatc agcttgctcc tcaggaaact tgccctggac ctggccaaca acagcatttg
     3121 ccttggagga tttggcatag gaaaaattga tccttcttgg cttggtcctt tatttccaga
     3181 taagtcatcc aatttaagga agcaaacaaa cacaggatct gtcctagcga ggaaagtgct
     3241 ccggtatcag atgagaaaca cccttcaaga aggcgtggcc tcaggcagtg acggcaagtt
     3301 ttctgaagac gcgctggcga aatttggaga atggaccttt attcccgact cttctatgga
     3361 cagtgtgttt ggccagagcg atgatctgga tagcgaaggc agcgagagct catttctcgt
     3421 gaagaggaag tccaactcaa ttagtgtagg ggaagtttac agagatctag ctctgcagcg
     3481 ctgctcacca aatgctcaga ggcattccaa ttcgctgggt cctgtttttg accatgaaga
     3541 cttactgaga cgaaaaagaa aaatactgtc ttcagatgag tctctcaggt cctcaaggct
     3601 gccgtcccat atgaggcaat cagatagctc ttcttccctg gcttctgaga gagaacacat
     3661 cacgtcgtta gacctatctg ccaacgaact caaagatatt gatgctctga gccagaagtg
     3721 ttgcctcagt agccacctgg aacatctcac caaactggaa cttcaccaga attcatccgg
     3781 agcaccagag tttctcttag gcgatggaag ttttggatcc gtttatcgag ctgcctacga
     3841 aggagaggaa gtggctgtga agatttttaa taagcacaca tctcttaggc tgttaagaca
     3901 agagttggtg gtcctttgtc accttcacca ccccagcctg atatccttgc tggcggctgg
     3961 tattcgtcct cggatgttgg taatggagtt ggcctccaaa ggttccttgg atcgcctgct
     4021 gcagcaggac aaagccagcc tcaccagaac cctccagcac aggatcgcgt tgcatgtggc
     4081 cgacggcctg aggtatctcc actcagccat gattatttac cgtgacctga agccccacaa
     4141 tgtgctgctt tttaccctgt atcccaatgc tgccatcatt gcgaagattg cggactacgg
     4201 gatcgcacag tactgctgca ggatgggaat aaagacatca gagggcaccc cagggttccg
     4261 ggcacctgaa gttgccaggg ggaatgtcat ttataaccaa caggccgatg tttattcttt
     4321 tggcttacta cttcacgata tttggacaac tgggagtagg attatggagg gtttgaggtt
     4381 cccaaatgag tttgatgagt tagccataca agggaagttg ccagatccag ttaaagaata
     4441 tggctgtgcc ccatggccta tggttgagaa gttaattaca aagtgtttga aagaaaatcc
     4501 tcaagaaaga cccacttctg cccaggtctt tgacattttg aattcggctg aattaatttg
     4561 cctcatgcga cacattttaa tacctaagaa catcattgtt gaatgcatgg ttgccacgaa
     4621 tctcaatagc aagagtgcga ctctctggtt gggatgtggg aacacagaaa aaggacagct
     4681 ttccttattt gacttaaaca cggaaagata cagctatgag gaagttgctg atagtagaat
     4741 actgtgcttg gctttggtgc atctcgctgc tgagaaagag agctgggttg tgtgcgggac
     4801 acagtctggg gctctcctgg tcatcaatgt tgaagaggag acaaagagac acaccctgga
     4861 aaagatgact gattctgtca cttgtttgca ttgcaattcc cttgccaagc agagcaagca
     4921 aagtaacttt cttttggtgg gaactgctga tggtaactta atgatatttg aagataaagc
     4981 cgttaagtgt aaaggagctg cccccttgaa gacactacac ataggcgatg tcagtacgcc
     5041 cctgatgtgc ctgagcgagt ccctgaattc atctgaaaga cacatcacat ggggagggtg
     5101 tggcacaaag gtcttctcct tttccaatga tttcaccatt cagaaactca tcgagacaaa
     5161 aaccaaccag ctgttttctt acgcagcttt cagcgattct aacatcatag cgctggcagt
     5221 agacacagcc ctgtatattg ccaagaaaaa cagccctgtc gtagaggtgt gggacaagaa
     5281 aacagaaaag ctctgtgaat taatagactg tgtgcacttc ttaaaggagg tgatggtaaa
     5341 actaaacaag gaatcgaaac atcagctgtc ctactctggg agggtgaagg ccctctgcct
     5401 gcagaagaac acggctctct ggatcggaac tggaggaggc cacatcttac tcctggatct
     5461 ttctactcgg cgagttatcc gcaccattca caatttctgt gattctgtga gagccatggc
     5521 cacagcacaa ttaggaagcc ttaagaatgt catgctggtt ttggggtaca agcggaagag
     5581 tacagagggt atccaagaac aaaaagagat acaatcttgt ttgtctattt gggacctcaa
     5641 tcttccacac gaggtgcaaa atttagaaaa acacattgaa gtaagaacag aattagctga
     5701 taaaatgagg aaaacatctg ttgaaggtac cgccctcgag caaaaactca tctcagaaga
     5761 ggatctgtag gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     5821 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5881 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5941 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6001 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     6061 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6121 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6181 ctttctcgcc acgttcgctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6241 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6301 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6361 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6421 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6481 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6541 ttgcatgcct gcaggtcggc cgccacgacc ggtgccgcca ccatcccctg acccacgccc
     6601 ctgacccctc acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc
     6661 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6721 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6781 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6841 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6901 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6961 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     7021 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     7081 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     7141 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     7201 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     7261 aaggagcgca cgaccccatg gctccgaccg aagccacccg gggcggcccc gccgaccccg
     7321 cacccgcccc cgaggcccac cgactctaga ggatcataat cagccatacc acatttgtag
     7381 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     7441 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7501 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7561 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     7621 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7681 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7741 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7801 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7861 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7921 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7981 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8041 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8101 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8161 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8221 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8281 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8341 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8401 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8461 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8521 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8581 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8641 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8701 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8761 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8821 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8881 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8941 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9001 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9061 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9121 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9181 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9241 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9301 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9361 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9421 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9481 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9541 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9601 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9661 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9721 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9781 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     9841 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     9901 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     9961 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtt
//
