LOCUS       sequence-318595-       14035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor 
            DNA; non-targeting crRNA with BsaI sites. Reverse (R) order 
            minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14035)
  AUTHORS   Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg SH
  TITLE     CRISPR RNA-guided integrases for high-efficiency, multiplexed 
            bacterial genome engineering.
  JOURNAL   Nat Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y.
  PUBMED    33230293
REFERENCE   2  (bases 1 to 14035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y."
COMMENT     Sequence Label: pSL1765 (pSPIN-R, R*MmeI)
FEATURES             Location/Qualifiers
     source          1..14035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      122..893
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2373..3167
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2427..2446)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3037..3056
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3611..3628
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3639..3673
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             5795..5806
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12108..12126)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12122..12169
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             12321..12980
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(12387..12406)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(13180..13197)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13180..13196)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13189..13211)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(join(13494..14035,1..121))
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 ccgccgtggg tttcctcgca ataagcccgg cccacgcctc atgcgctttg cgttccgttt
       61 gcacccagtg accgggcttg ttcttggctt gaatgccgat ttctctggac tgcgtggcca
      121 tgcttatctc catgcggtag gggtgccgca cggttgcggc accatgcgca atcagctgca
      181 acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa gtggcagtca attacagatt
      241 ttctttaacc tacgcaatga gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc
      301 ccttttttaa gttgttgatt tttaagtctt tcgcatttcg ccctatatct agttctttgg
      361 tgcccaaaga agggcacccc tgcggggttc ccccacgcct tcggcgcggc tccccctccg
      421 gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc ggccttcggc cttgcccaag
      481 gtggcgctgc ccccttggaa cccccgcact cgccgccgtg aggctcgggg ggcaggcggg
      541 cgggcttcgc ccttcgactg cccccactcg cataggcttg ggtcgttcca ggcgcgtcaa
      601 ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt ggtgtccaac
      661 cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa attaaaaaaa
      721 ttgatggggc aaggccgcag gccgcgcagt tggagccggt gggtatgtgg tcgaaggctg
      781 ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct gtccatcagc
      841 ttgtccagca gggttgtcca cgggccgagc gaagcgagcc agccggtggc cgctcgcggc
      901 catcgtccac atatccacgg gctggcaagg gagcgcagcg accgcgcagg gcgaagcccg
      961 gagagcaagc ccgtagggcg ccgcagccgc cgtaggcggt cacgactttg cgaagcaaag
     1021 tctagtgagt atactcaagc attgagtggc ccgccggagg caccgccttg cgctgccccc
     1081 gtcgagccgg ttggacacca aaagggaggg gcaggcatgg cggcatacgc gatcatgcga
     1141 tgcaagaagc tggcgaaaat gggcaacgtg gcggccagtc tcaagcacgc ctaccgcgag
     1201 cgcgagacgc ccaacgctga cgccagcagg acgccagaga acgagcactg ggcggccagc
     1261 agcaccgatg aagcgatggg ccgactgcgc gagttgctgc cagagaagcg gcgcaaggac
     1321 gctgtgttgg cggtcgagta cgtcatgacg gccagcccgg aatggtggaa gtcggccagc
     1381 caagaacagc aggcggcgtt cttcgagaag gcgcacaagt ggctggcgga caagtacggg
     1441 gcggatcgca tcgtgacggc cagcatccac cgtgacgaaa ccagcccgca catgaccgcg
     1501 ttcgtggtgc cgctgacgca ggacggcagg ctgtcggcca aggagttcat cggcaacaaa
     1561 gcgcagatga cccgcgacca gaccacgttt gcggccgctg tggccgatct agggctgcaa
     1621 cggggcatcg agggcagcaa ggcacgtcac acgcgcattc aggcgttcta cgaggccctg
     1681 gagcggccac cagtgggcca cgtcaccatc agcccgcaag cggtcgagcc acgcgcctat
     1741 gcaccgcagg gattggccga aaagctggga atctcaaagc gcgttgagac gccggaagcc
     1801 gtggccgacc ggctgacaaa agcggttcgg caggggtatg agcctgccct acaggccgcc
     1861 gcaggagcgc gtgagatgcg caagaaggcc gatcaagccc aagagacggc ccgtgacctt
     1921 cgggagcgcc tgaagcccgt tctggacgcc ctggggccgt tgaatcggga tatgcaggcc
     1981 aaggccgccg cgatcatcaa ggccgtgggc gaaaagctgc tgacggaaca gcgggaagtc
     2041 cagcgccaga aacaggccca gcgccagcag gaacgcgggc gcgcacattt ccccgaaaag
     2101 tgccacctgg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     2161 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     2221 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     2281 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     2341 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     2401 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     2461 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     2521 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     2581 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     2641 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     2701 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     2761 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     2821 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     2881 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     2941 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3001 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3061 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3121 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3181 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     3241 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     3301 ccagcgcggg gatctcatgc tggagttctt cgcccacccc catgggcaaa tattatacgc
     3361 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     3421 tccatgtcgg cagaatgctt aatgaattac aacagttttt atgcatgcgc ccaatacgca
     3481 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3541 actggtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3601 gaccgagcgc agcgagtcag tgagcgagga cacaagcttt gacagctagc tcagtcctag
     3661 gtataatact agcgtcgaca ggatccgaat tcgagctcgg tataaggaga tataccatgg
     3721 cgacaagttt acctacgccc tcagcaatta cgacttcggc gttagagtat gcattccata
     3781 ctcccgctcg caatctaacg aaatctcgcg gaaaaaacat tcatcgttat gtcagtgtaa
     3841 agatgagtaa gaggattacg gtagaatcta ctctagagtg tgatgcctgc tatcactttg
     3901 attttgagcc aagtattgtt cgcttttgcg ctcaaccgat tcgattttta tattatctca
     3961 atggtcagtc tcactcctat gttcctgact ttctagttca atttgatacc aacgagtttg
     4021 ttctatatga agtaaagtca gcttatgcta agaacaaacc tgattttgat gttgaatggg
     4081 aggcgaaagt aaaagcagca actgaactag ggctagaatt ggagcttgtt gaagagagtg
     4141 atattaggga tacggttgta ttaaataatc ttaagcgcat gcatcgttat gcttcgaaag
     4201 atgagctgaa taacgtacat aactctctct taaaaataat aaagtacaat ggcgcccaat
     4261 ctgcaagatg cttgggagaa cagttgggtt taaaaggccg aactgtttta ccaattttgt
     4321 gcgatttgct gtcaaggtgt ttactcgata cacgtttgga taagcctcta tctcttgaat
     4381 ctcgatttga gttggccagt tatggctaag aaagggttct caagtttcca tagaaaagca
     4441 gtctcttccc aagatacgct tgaatcgata gagcttgtct ctagcgctaa ttgtctagaa
     4501 agtgttacgt atcaagatat atcagcattt cccgaaacaa ttgcggtaga gattaatttc
     4561 cgattaagca ttcttcgctt tttagcgcga aagtgcgaaa ccattgtggc caaatcaatt
     4621 gaaccacatc gtgtagagct acagcaaaac tatagtagaa aaatacccag tgcaataacg
     4681 atatatcgat ggtggcttgc ttttcgaaaa tcagactaca accccattag cttagcacct
     4741 aatatcaagg atagaggtaa tagagagaca aaagtgtcaa cagttgttga ttctattatg
     4801 gaacaggcag ttgaaagagt tatatctgga cgaaaagtca atgttagctc tgcatataaa
     4861 cgtgttcgac gaaaagttcg tcaatacaat ctaactcatg gaacgaaata cacgtatcct
     4921 aagtacgaat ctgtaagaaa gcgagtaaaa aagaaaaccc catttgagtt attagccgca
     4981 gggaaagggg agagagtagc taagagagag tttcgccgaa tgggaaaaaa gatcctcacg
     5041 tctagcgtgc tagagagggt tgaaatagat cacactgtcg ttgacctttt tgcagtacac
     5101 gaagagtatc gaatcccatt gggccgacct tggcttactc aattggttga ttgttacagt
     5161 aaagctgtaa tcggttttta tttaggtttc gagcctccta gctatgtgtc ggtttccctt
     5221 gcacttaaga atgcaataca acgcaaagat gacttaatct cctcgtatga atcgatcgag
     5281 aatgaatggc tatgttatgg catcccagac ctactcgtaa ctgataatgg taaagagttt
     5341 ttgtcgaaag catttgatca agcatgtgaa tcactattga tcaatgtgca tcaaaataaa
     5401 gttgagacgc ccgacaacaa acctcatgtt gaacgtaact acgggactat taatacttct
     5461 ctgttagacg atttacctgg gaaatccttc agccagtacc ttcaaagaga agggtacgac
     5521 tctgtgggag aagctaccct tacactcaat gagattagag aaatttactt aatttggttg
     5581 gtggatattt atcataaaaa acccaatcag agaggcacta attgtcctaa tgttgcgtgg
     5641 aaaaagggtt gtcaagaatg ggaaccagag gagttctctg gttctaaaga cgaattagac
     5701 tttaaatttg ctattgttga ttacaaacaa cttactaaag tagggataac tgtctacaaa
     5761 gaactgagtt atagcaatga ccgtttagct gaatatagag ggaagaaagg aaaccataaa
     5821 gttcagttca agtataaccc tgagtgtatg gcagttattt gggtgttgga tgaggatatg
     5881 aatgagtact ttacagttaa tgcgattgac tacgaatatg caagtagagt atcactttgg
     5941 caacataaat ataacatgaa atatcaagca gaactaaatt cagcagaata tgatgaggac
     6001 aaggaaattg atgcagaaat aaaaattgaa gaaatcgcag atcgttcaat tgttaagact
     6061 aacaaaatca gagctcggag gcgtggcgct aggcatcaag agaatagcgc aagggctaag
     6121 tcaatcagta atgcgaaccc ggcctcgata caaaaacatg aagatgaaat cgttagtgca
     6181 gataatgacg attgggatat tgattatgtc tgagaaatcg tcaaatgagt gaaacgcgtg
     6241 aggctcgtat atcaagagct aaaagggcat ttgtatccac accgagcgtt aggaaaatct
     6301 tgagttacat ggatagatgt agagatctat cagacctaga gtctgagcct acatgcatga
     6361 tggtctatgg tgcttcgggt gtaggtaaaa cgaccgtcat caagaaatac ttaaatcaaa
     6421 acagaagaga gtccgaagcc gggggcgata taataccggt tttgcatatt gagttgccag
     6481 acaatgcgaa gccagtagat gcagcaaggg aattgctggt tgaaatgggt gacccgctag
     6541 cactttatga aactgactta gctagattga cgaaaagact gactgaatta atccctgcgg
     6601 tcggcgtgaa gctgattatt atcgatgagt tccaacattt ggtggaagaa aggtcaaatc
     6661 gggttcttac ccaagtaggt aattggctaa aaatgatact taacaaaacg aaatgtccaa
     6721 ttgttatatt tggtatgcca tactcaaaag ttgtactgca agcaaactcg caacttcacg
     6781 ggcgattttc cattcaggtt gaactgcgcc cctttagcta ccagggaggt agaggtgtat
     6841 ttaaaacttt tttggaatac cttgataaag ccctaccttt tgaaaaacag gctggcttag
     6901 ccaacgaaag tttgcagaaa aaattgtatg cattctctca gggaaacatg cgttcgttga
     6961 gaaaccttat ttatcaagca tctatcgaag caattgataa tcagcatgag acgataaccg
     7021 aagaagattt cgtttttgca tcgaagttga catcgggcga taaacccaac tcatggaaaa
     7081 atccttttga ggagggtgtt gaggtaacag aagatatgtt acgaccgcca ccaaaagata
     7141 ttggttggga ggactatttg agacattcaa ccccgagagt gagtaaacca ggtagaaata
     7201 aaaacttttt cgaataacct aggctgctgg aaggagatat acatatgttt ttgcaaagac
     7261 ctaaacctta cagcgatgaa agtttagaaa gtttctttat ccgagtggct aacaaaaatg
     7321 gctacggtga tgtccatcgc ttcctagaag ccactaaacg attccttcaa gacattgacc
     7381 ataatggcta tcaaaccttt ccgactgata taactcggat aaacccatac tcagctaaaa
     7441 acagttccag cgcacgaact gcgtcattcc tgaaacttgc acaattgaca tttaatgaac
     7501 cgccagagct acttgggttg gcaattaaca gaacaaacat gaaatactcg ccgtcaacta
     7561 gcgcggttgt tagaggtgca gaagtctttc ctcgcagttt actacggacg cactccatcc
     7621 cctgctgtcc tttgtgtctg cgagaaaatg gctacgcctc ctacctttgg cactttcagg
     7681 ggtacgaata ctgccacagc cataacgtac ctttaattac cacttgtagc tgtggtaagg
     7741 agtttgacta ccgagtatct gggttaaagg gcatttgctg caaatgcaag gagcctatca
     7801 ccttaaccag cagggagaac ggtcatgagg cagcgtgtac tgtttcaaac tggcttgctg
     7861 gccatgaatc taaacctctg ccaaatcttc ctaaaagcta ccgatggggt ttagttcatt
     7921 ggtggatggg tattaaagat agcgagttcg atcacttttc gttcgttcaa tttttctcaa
     7981 actggccaag gtcattccac tcgataatcg aagatgaagt agagttcaac cttgagcatg
     8041 ctgttgtcag cacgtctgaa ttacgactaa aagatcttct tggtcgattg tttttcggtt
     8101 caattcggtt acctgagcgg aatcttcaac acaatatcat ccttggtgag cttctctgct
     8161 atttagaaaa tcgtttatgg caagacaagg gattaatcgc caacctcaaa atgaacgcgt
     8221 tagaggcgac tgtaatgtta aattgtagcc tcgatcagat tgcatcaatg gttgaacaac
     8281 gcatcttgaa gccaaatcga aaaagcaagc ccaacagccc tcttgatgtt accgattatc
     8341 tatttcattt cggcgatatt ttctgtcttt ggttagctga gttccaaagc gatgagttta
     8401 accgttcgtt ttatgtgtcg aggtggtaaa tgcaaactct gaaagaacta atcgcatcca
     8461 atcctgacga cttaacaact gaacttaaga gagcatttcg tccactcaca ccgcatattg
     8521 caattgatgg taatgaactt gacgcactga cgatattagt caatttaacc gataagactg
     8581 atgatcagaa agacctgctc gatcgagcca aatgcaagca aaaacttcga gatgaaaaat
     8641 ggtgggctag ctgcataaat tgcgttaatt acagacaaag ccataaccca aaattcccgg
     8701 atatacgttc tgaaggcgtg attcgaaccc aagccctggg tgaattaccg agtttcctac
     8761 tctcgtcctc aaaaatccca ccataccatt ggtcatatag ccatgattca aaatacgtca
     8821 acaaaagcgc attcctcacc aatgagtttt gttgggatgg tgagatctca tgtttgggtg
     8881 agcttcttaa agatgcagat cacccacttt ggaatacttt gaaaaagtta ggttgttctc
     8941 aaaaaacttg caaagcaatg gcaaaacaac tagctgatat tactctcacg actatcaatg
     9001 tcacactcgc accaaattac ctgactcaaa tatctctccc cgatagtgat acatcctaca
     9061 tttcactctc tcctgttgca tcgctatcga tgcaaagcca ctttcatcag aggctccaag
     9121 atgaaaatcg gcatagcgcg ataacgcggt ttagccgaac taccaacatg ggggtcactg
     9181 cgatgacatg tggcggcgca tttagaatgt taaagtctgg cgctaagttt tccagccccc
     9241 ctcatcaccg attaaacagt aaacgaagtt ggttgacgtc agagcatgtt cagtcattaa
     9301 aacagtacca gcgcctcaac aaaagcctca tacctgaaaa ctctcggatt gcactccgta
     9361 gaaaatacaa aatcgagctt caaaatatgg tcagatcttg gtttgcaatg caagaccata
     9421 cccttgattc gaacatactt atccaacatt tgaatcatga cctatcttac ttaggagcca
     9481 caaaacgttt tgcatacgat ccagcgatga ccaagctctt tactgagctt ttgaaacgag
     9541 agttatcaaa ttcaatcaat aatggtgagc aacacactaa tggatcgttt ttagtcctac
     9601 cgaatatcag ggtttgtggc gcaacagctt taagctcccc ggtaacggtg gggattccat
     9661 cacttacagc tttctttggc ttcgttcacg catttgaacg gaatataaat cgcaccacct
     9721 catcgtttcg tgttgaatcc tttgcgatat gcgtccatca actacatgtc gagaagcgag
     9781 gtttgacagc agagtttgtg gaaaaaggcg acgggactat atccgctccc gcgacccggg
     9841 atgactggca gtgtgatgtc gtatttagcc ttattttgaa caccaacttt gctcaacata
     9901 ttgaccaaga tacgttagtt acatcactac caaagcgatt ggctcggggt tcagcaaaaa
     9961 ttgcgattga tgactttaaa catatcaact cattctcgac attagaaaca gcgatcgaat
    10021 ctctgccaat agaagctggt aggtggttat cactttacgc acagtcaaac aataatctaa
    10081 gtgatctatt agcagccatg acagaggacc atcagctcat ggcaagctgc gtcggttacc
    10141 acttgttaga agagcccaaa gataaaccaa actccctcag aggttacaaa cacgctatcg
    10201 ccgagtgcat cattggactc attaactcaa tcacctttag ctcagagact gatcccaaca
    10261 caatcttttg gtcgctaaag aactatcaaa actacctagt ggtacagcca aggagtatca
    10321 acgatgaaac taccgacaaa tctagcctat gagcgctcta tcgacccatc agatgtctgt
    10381 ttttttgtcg tctggcccga tgatagaaaa acacctttaa cctacaattc tcgtactctg
    10441 ctcgggcaaa tggaagcggc atcattagcc tatgatgtct caggtcaacc aataaaaagt
    10501 gccaccgctg aggcgttagc tcaagggaac cctcatcaag ttgatttctg ccacgttcca
    10561 tacggtgcga gtcatattga atgcagtttc tccgtctcgt tttcttctga actacgtcaa
    10621 ccatataagt gtaactcaag caaagttaaa caaacgctag tgcaattagt cgagctctac
    10681 gaaacgaaaa tcggctggac tgagctagca acccgatatt tgatgaacat ttgcaacggt
    10741 aaatggctgt ggaaaaatac ccgtaaagcc tattgctgga acattgtact tacaccttgg
    10801 ccttggaacg gggaaaaggt tggatttgaa gatattcgta ctaactacac ctcacggcaa
    10861 gactttaaaa ataataaaaa ttggtctgct atagttgaaa tgatcaaaac cgcattttct
    10921 agtactgatg ggctggcgat atttgaagtc agggccacct tgcacttgcc aacgaatgct
    10981 atggtgcggc caagccaagt tttcacagaa aaagaaagtg gcagtaaaag taaatctaaa
    11041 actcaaaaca gtcgagtttt tcagagtaca actattgatg gtgaacgatc gccaatacta
    11101 ggggccttta aaacgggagc agctattgca accattgacg actggtatcc tgaagccact
    11161 gagccactaa gggtcggacg gtttggggtt catcgcgaag atgtcacttg ctaccgtcat
    11221 ccgtctaccg gaaaagattt tttctcgata ttacaacaag cagagcacta tattgaagtg
    11281 ttgagcgcca acaaaactcc cgctcaagaa actatcaacg acatgcactt tttaatggct
    11341 aacctgatta agggtgggat gttccagcat aaaggagact gactgtgaaa tggtattata
    11401 agacaatcac ctttctgcca gagttgtgca acaacgagtc actggctgca aagtgtctcc
    11461 gcgttctgca tggatttaac tatcagtatg agacacgaaa tatcggcgtt tcatttccgc
    11521 tttggtgtga tgcaacggtt ggaaaaaaga tttcatttgt cagcaagaac aagatagaac
    11581 tcgacttact acttaaacaa cactatttcg tccaaatgga acaacttcaa tactttcata
    11641 tatccaacac tgttctcgtc ccagaagatt gtacatacgt ttcctttaga cgctgtcaat
    11701 ctatagataa gctcacagca gcagggctgg caaggaaaat cagacgcctg gagaaacgtg
    11761 ctctatctag aggcgagcaa tttgacccat catcttttgc tcaaaaagag catactgcaa
    11821 tagcgcacta ccactcactt ggggagtcca gcaaacagac gaaccgcaac tttcgactca
    11881 atatcaggat gctctcggag caaccccgtg aggggaactc gatttttagt agctatggct
    11941 tatcaaattc agaaaactcg tttcagcctg tacccttaat ctgacgatat accatgggtg
    12001 aactgccgag taggtagctg ataacgagac ctctgtcttg tcagctaggg tggtctcgtg
    12061 aactgccgag taggtagctg ataacggatc cgaattcgct gctgccaccg ctgagcaata
    12121 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca
    12181 ggcatgttgg aacaaccata aaatgataat tacacccata aattgataat tatcacaccc
    12241 ataaattgat attgcctctt catggtctaa acttcagtaa gtttacgaca ttttcatgaa
    12301 gaggtcattt ccggggatcc atggagaaaa aaatcactgg atataccacc gttgatatat
    12361 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
    12421 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
    12481 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggagttcc
    12541 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
    12601 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
    12661 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
    12721 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
    12781 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcactatgg
    12841 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
    12901 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
    12961 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttct agagtcctta
    13021 ctgcagtagt tttgctgaaa tactcgattc acaaaaatat caacttatgg ttgttttgtg
    13081 agatatcaat atatggttgt tttgtggtta agttgctgat tataaataat tattaaatat
    13141 cactttatgg ttgcatcaac aggtaccgag ctcgaattca ctggccgtcg ttttacaacg
    13201 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcggcca gtttgctcag
    13261 gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc ccagagcctg
    13321 ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc
    13381 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgaggcggc
    13441 tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa gtgctaccgg
    13501 cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc
    13561 tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag
    13621 gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg
    13681 ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc
    13741 cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac
    13801 aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac
    13861 acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg
    13921 actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg
    13981 accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattg
//
