LOCUS       sequence-318598-       11860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Effector expression for V. cholerae CAST system, encodes all protein
            and RNA components. Entry vector encodes non-targeting crRNA, with 
            BsaI sites for spacer cloning. pCDF backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11860)
  AUTHORS   Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg SH
  TITLE     CRISPR RNA-guided integrases for high-efficiency, multiplexed 
            bacterial genome engineering.
  JOURNAL   Nat Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y.
  PUBMED    33230293
REFERENCE   2  (bases 1 to 11860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y."
COMMENT     Sequence Label: pSL1777 (pEffector)
FEATURES             Location/Qualifiers
     source          1..11860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(157..1239)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1201..1220
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1240..1317)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1426..1443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1454..1488
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             8470..8481
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9916..9934)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9930..9977
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(10023..10040)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10023..10039)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10032..10054)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(10138..10929)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(10930..11021)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11069..11807)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc
       61 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
      121 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      181 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      241 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
      301 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
      361 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
      421 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
      481 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
      541 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
      601 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
      661 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
      721 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
      781 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
      841 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
      901 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
      961 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     1021 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
     1081 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     1141 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     1201 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     1261 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
     1321 gatctcgacg ctctccctta tgcgactcct gcattaggaa tggataaccg tattaccgcc
     1381 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1441 gaggacacaa gctttgacag ctagctcagt cctaggtata atactagcgt cgacgtggag
     1501 atataccatg ggtgaactgc cgagtaggta gctgataacg agacctctgt cttgtcagct
     1561 agggtggtct cgtgaactgc cgagtaggta gctgataacg gatccgaatt cgagcgaagg
     1621 agatatacat atgtttttgc aaagacctaa accttacagc gatgaaagtt tagaaagttt
     1681 ctttatccga gtggctaaca aaaatggcta cggtgatgtc catcgcttcc tagaagccac
     1741 taaacgattc cttcaagaca ttgaccataa tggctatcaa acctttccga ctgatataac
     1801 tcggataaac ccatactcag ctaaaaacag ttccagcgca cgaactgcgt cattcctgaa
     1861 acttgcacaa ttgacattta atgaaccgcc agagctactt gggttggcaa ttaacagaac
     1921 aaacatgaaa tactcgccgt caactagcgc ggttgttaga ggtgcagaag tctttcctcg
     1981 cagtttacta cggacgcact ccatcccctg ctgtcctttg tgtctgcgag aaaatggcta
     2041 cgcctcctac ctttggcact ttcaggggta cgaatactgc cacagccata acgtaccttt
     2101 aattaccact tgtagctgtg gtaaggagtt tgactaccga gtatctgggt taaagggcat
     2161 ttgctgcaaa tgcaaggagc ctatcacctt aaccagcagg gagaacggtc atgaggcagc
     2221 gtgtactgtt tcaaactggc ttgctggcca tgaatctaaa cctctgccaa atcttcctaa
     2281 aagctaccga tggggtttag ttcattggtg gatgggtatt aaagatagcg agttcgatca
     2341 cttttcgttc gttcaatttt tctcaaactg gccaaggtca ttccactcga taatcgaaga
     2401 tgaagtagag ttcaaccttg agcatgctgt tgtcagcacg tctgaattac gactaaaaga
     2461 tcttcttggt cgattgtttt tcggttcaat tcggttacct gagcggaatc ttcaacacaa
     2521 tatcatcctt ggtgagcttc tctgctattt agaaaatcgt ttatggcaag acaagggatt
     2581 aatcgccaac ctcaaaatga acgcgttaga ggcgactgta atgttaaatt gtagcctcga
     2641 tcagattgca tcaatggttg aacaacgcat cttgaagcca aatcgaaaaa gcaagcccaa
     2701 cagccctctt gatgttaccg attatctatt tcatttcggc gatattttct gtctttggtt
     2761 agctgagttc caaagcgatg agtttaaccg ttcgttttat gtgtcgaggt ggtaaatgca
     2821 aactctgaaa gaactaatcg catccaatcc tgacgactta acaactgaac ttaagagagc
     2881 atttcgtcca ctcacaccgc atattgcaat tgatggtaat gaacttgacg cactgacgat
     2941 attagtcaat ttaaccgata agactgatga tcagaaagac ctgctcgatc gagccaaatg
     3001 caagcaaaaa cttcgagatg aaaaatggtg ggctagctgc ataaattgcg ttaattacag
     3061 acaaagccat aacccaaaat tcccggatat acgttctgaa ggcgtgattc gaacccaagc
     3121 cctgggtgaa ttaccgagtt tcctactctc gtcctcaaaa atcccaccat accattggtc
     3181 atatagccat gattcaaaat acgtcaacaa aagcgcattc ctcaccaatg agttttgttg
     3241 ggatggtgag atctcatgtt tgggtgagct tcttaaagat gcagatcacc cactttggaa
     3301 tactttgaaa aagttaggtt gttctcaaaa aacttgcaaa gcaatggcaa aacaactagc
     3361 tgatattact ctcacgacta tcaatgtcac actcgcacca aattacctga ctcaaatatc
     3421 tctccccgat agtgatacat cctacatttc actctctcct gttgcatcgc tatcgatgca
     3481 aagccacttt catcagaggc tccaagatga aaatcggcat agcgcgataa cgcggtttag
     3541 ccgaactacc aacatggggg tcactgcgat gacatgtggc ggcgcattta gaatgttaaa
     3601 gtctggcgct aagttttcca gcccccctca tcaccgatta aacagtaaac gaagttggtt
     3661 gacgtcagag catgttcagt cattaaaaca gtaccagcgc ctcaacaaaa gcctcatacc
     3721 tgaaaactct cggattgcac tccgtagaaa atacaaaatc gagcttcaaa atatggtcag
     3781 atcttggttt gcaatgcaag accataccct tgattcgaac atacttatcc aacatttgaa
     3841 tcatgaccta tcttacttag gagccacaaa acgttttgca tacgatccag cgatgaccaa
     3901 gctctttact gagcttttga aacgagagtt atcaaattca atcaataatg gtgagcaaca
     3961 cactaatgga tcgtttttag tcctaccgaa tatcagggtt tgtggcgcaa cagctttaag
     4021 ctccccggta acggtgggga ttccatcact tacagctttc tttggcttcg ttcacgcatt
     4081 tgaacggaat ataaatcgca ccacctcatc gtttcgtgtt gaatcctttg cgatatgcgt
     4141 ccatcaacta catgtcgaga agcgaggttt gacagcagag tttgtggaaa aaggcgacgg
     4201 gactatatcc gctcccgcga cccgggatga ctggcagtgt gatgtcgtat ttagccttat
     4261 tttgaacacc aactttgctc aacatattga ccaagatacg ttagttacat cactaccaaa
     4321 gcgattggct cggggttcag caaaaattgc gattgatgac tttaaacata tcaactcatt
     4381 ctcgacatta gaaacagcga tcgaatctct gccaatagaa gctggtaggt ggttatcact
     4441 ttacgcacag tcaaacaata atctaagtga tctattagca gccatgacag aggaccatca
     4501 gctcatggca agctgcgtcg gttaccactt gttagaagag cccaaagata aaccaaactc
     4561 cctcagaggt tacaaacacg ctatcgccga gtgcatcatt ggactcatta actcaatcac
     4621 ctttagctca gagactgatc ccaacacaat cttttggtcg ctaaagaact atcaaaacta
     4681 cctagtggta cagccaagga gtatcaacga tgaaactacc gacaaatcta gcctatgagc
     4741 gctctatcga cccatcagat gtctgttttt ttgtcgtctg gcccgatgat agaaaaacac
     4801 ctttaaccta caattctcgt actctgctcg ggcaaatgga agcggcatca ttagcctatg
     4861 atgtctcagg tcaaccaata aaaagtgcca ccgctgaggc gttagctcaa gggaaccctc
     4921 atcaagttga tttctgccac gttccatacg gtgcgagtca tattgaatgc agtttctccg
     4981 tctcgttttc ttctgaacta cgtcaaccat ataagtgtaa ctcaagcaaa gttaaacaaa
     5041 cgctagtgca attagtcgag ctctacgaaa cgaaaatcgg ctggactgag ctagcaaccc
     5101 gatatttgat gaacatttgc aacggtaaat ggctgtggaa aaatacccgt aaagcctatt
     5161 gctggaacat tgtacttaca ccttggcctt ggaacgggga aaaggttgga tttgaagata
     5221 ttcgtactaa ctacacctca cggcaagact ttaaaaataa taaaaattgg tctgctatag
     5281 ttgaaatgat caaaaccgca ttttctagta ctgatgggct ggcgatattt gaagtcaggg
     5341 ccaccttgca cttgccaacg aatgctatgg tgcggccaag ccaagttttc acagaaaaag
     5401 aaagtggcag taaaagtaaa tctaaaactc aaaacagtcg agtttttcag agtacaacta
     5461 ttgatggtga acgatcgcca atactagggg cctttaaaac gggagcagct attgcaacca
     5521 ttgacgactg gtatcctgaa gccactgagc cactaagggt cggacggttt ggggttcatc
     5581 gcgaagatgt cacttgctac cgtcatccgt ctaccggaaa agattttttc tcgatattac
     5641 aacaagcaga gcactatatt gaagtgttga gcgccaacaa aactcccgct caagaaacta
     5701 tcaacgacat gcacttttta atggctaacc tgattaaggg tgggatgttc cagcataaag
     5761 gagactgact gtgaaatggt attataagac aatcaccttt ctgccagagt tgtgcaacaa
     5821 cgagtcactg gctgcaaagt gtctccgcgt tctgcatgga tttaactatc agtatgagac
     5881 acgaaatatc ggcgtttcat ttccgctttg gtgtgatgca acggttggaa aaaagatttc
     5941 atttgtcagc aagaacaaga tagaactcga cttactactt aaacaacact atttcgtcca
     6001 aatggaacaa cttcaatact ttcatatatc caacactgtt ctcgtcccag aagattgtac
     6061 atacgtttcc tttagacgct gtcaatctat agataagctc acagcagcag ggctggcaag
     6121 gaaaatcaga cgcctggaga aacgtgctct atctagaggc gagcaatttg acccatcatc
     6181 ttttgctcaa aaagagcata ctgcaatagc gcactaccac tcacttgggg agtccagcaa
     6241 acagacgaac cgcaactttc gactcaatat caggatgctc tcggagcaac cccgtgaggg
     6301 gaactcgatt tttagtagct atggcttatc aaattcagaa aactcgtttc agcctgtacc
     6361 cttaatctga cctttaataa ggagatatac catggcgaca agtttaccta cgccctcagc
     6421 aattacgact tcggcgttag agtatgcatt ccatactccc gctcgcaatc taacgaaatc
     6481 tcgcggaaaa aacattcatc gttatgtcag tgtaaagatg agtaagagga ttacggtaga
     6541 atctactcta gagtgtgatg cctgctatca ctttgatttt gagccaagta ttgttcgctt
     6601 ttgcgctcaa ccgattcgat ttttatatta tctcaatggt cagtctcact cctatgttcc
     6661 tgactttcta gttcaatttg ataccaacga gtttgttcta tatgaagtaa agtcagctta
     6721 tgctaagaac aaacctgatt ttgatgttga atgggaggcg aaagtaaaag cagcaactga
     6781 actagggcta gaattggagc ttgttgaaga gagtgatatt agggatacgg ttgtattaaa
     6841 taatcttaag cgcatgcatc gttatgcttc gaaagatgag ctgaataacg tacataactc
     6901 tctcttaaaa ataataaagt acaatggcgc ccaatctgca agatgcttgg gagaacagtt
     6961 gggtttaaaa ggccgaactg ttttaccaat tttgtgcgat ttgctgtcaa ggtgtttact
     7021 cgatacacgt ttggataagc ctctatctct tgaatctcga tttgagttgg ccagttatgg
     7081 ctaagaaagg gttctcaagt ttccatagaa aagcagtctc ttcccaagat acgcttgaat
     7141 cgatagagct tgtctctagc gctaattgtc tagaaagtgt tacgtatcaa gatatatcag
     7201 catttcccga aacaattgcg gtagagatta atttccgatt aagcattctt cgctttttag
     7261 cgcgaaagtg cgaaaccatt gtggccaaat caattgaacc acatcgtgta gagctacagc
     7321 aaaactatag tagaaaaata cccagtgcaa taacgatata tcgatggtgg cttgcttttc
     7381 gaaaatcaga ctacaacccc attagcttag cacctaatat caaggataga ggtaatagag
     7441 agacaaaagt gtcaacagtt gttgattcta ttatggaaca ggcagttgaa agagttatat
     7501 ctggacgaaa agtcaatgtt agctctgcat ataaacgtgt tcgacgaaaa gttcgtcaat
     7561 acaatctaac tcatggaacg aaatacacgt atcctaagta cgaatctgta agaaagcgag
     7621 taaaaaagaa aaccccattt gagttattag ccgcagggaa aggggagaga gtagctaaga
     7681 gagagtttcg ccgaatggga aaaaagatcc tcacgtctag cgtgctagag agggttgaaa
     7741 tagatcacac tgtcgttgac ctttttgcag tacacgaaga gtatcgaatc ccattgggcc
     7801 gaccttggct tactcaattg gttgattgtt acagtaaagc tgtaatcggt ttttatttag
     7861 gtttcgagcc tcctagctat gtgtcggttt cccttgcact taagaatgca atacaacgca
     7921 aagatgactt aatctcctcg tatgaatcga tcgagaatga atggctatgt tatggcatcc
     7981 cagacctact cgtaactgat aatggtaaag agtttttgtc gaaagcattt gatcaagcat
     8041 gtgaatcact attgatcaat gtgcatcaaa ataaagttga gacgcccgac aacaaacctc
     8101 atgttgaacg taactacggg actattaata cttctctgtt agacgattta cctgggaaat
     8161 ccttcagcca gtaccttcaa agagaagggt acgactctgt gggagaagct acccttacac
     8221 tcaatgagat tagagaaatt tacttaattt ggttggtgga tatttatcat aaaaaaccca
     8281 atcagagagg cactaattgt cctaatgttg cgtggaaaaa gggttgtcaa gaatgggaac
     8341 cagaggagtt ctctggttct aaagacgaat tagactttaa atttgctatt gttgattaca
     8401 aacaacttac taaagtaggg ataactgtct acaaagaact gagttatagc aatgaccgtt
     8461 tagctgaata tagagggaag aaaggaaacc ataaagttca gttcaagtat aaccctgagt
     8521 gtatggcagt tatttgggtg ttggatgagg atatgaatga gtactttaca gttaatgcga
     8581 ttgactacga atatgcaagt agagtatcac tttggcaaca taaatataac atgaaatatc
     8641 aagcagaact aaattcagca gaatatgatg aggacaagga aattgatgca gaaataaaaa
     8701 ttgaagaaat cgcagatcgt tcaattgtta agactaacaa aatcagagct cggaggcgtg
     8761 gcgctaggca tcaagagaat agcgcaaggg ctaagtcaat cagtaatgcg aacccggcct
     8821 cgatacaaaa acatgaagat gaaatcgtta gtgcagataa tgacgattgg gatattgatt
     8881 atgtctgaga aatcgtcaaa tgagtgaaac gcgtgaggct cgtatatcaa gagctaaaag
     8941 ggcatttgta tccacaccga gcgttaggaa aatcttgagt tacatggata gatgtagaga
     9001 tctatcagac ctagagtctg agcctacatg catgatggtc tatggtgctt cgggtgtagg
     9061 taaaacgacc gtcatcaaga aatacttaaa tcaaaacaga agagagtccg aagccggggg
     9121 cgatataata ccggttttgc atattgagtt gccagacaat gcgaagccag tagatgcagc
     9181 aagggaattg ctggttgaaa tgggtgaccc gctagcactt tatgaaactg acttagctag
     9241 attgacgaaa agactgactg aattaatccc tgcggtcggc gtgaagctga ttattatcga
     9301 tgagttccaa catttggtgg aagaaaggtc aaatcgggtt cttacccaag taggtaattg
     9361 gctaaaaatg atacttaaca aaacgaaatg tccaattgtt atatttggta tgccatactc
     9421 aaaagttgta ctgcaagcaa actcgcaact tcacgggcga ttttccattc aggttgaact
     9481 gcgccccttt agctaccagg gaggtagagg tgtatttaaa acttttttgg aataccttga
     9541 taaagcccta ccttttgaaa aacaggctgg cttagccaac gaaagtttgc agaaaaaatt
     9601 gtatgcattc tctcagggaa acatgcgttc gttgagaaac cttatttatc aagcatctat
     9661 cgaagcaatt gataatcagc atgagacgat aaccgaagaa gatttcgttt ttgcatcgaa
     9721 gttgacatcg ggcgataaac ccaactcatg gaaaaatcct tttgaggagg gtgttgaggt
     9781 aacagaagat atgttacgac cgccaccaaa agatattggt tgggaggact atttgagaca
     9841 ttcaaccccg agagtgagta aaccaggtag aaataaaaac tttttcgaat aacctaggct
     9901 gctgcgctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     9961 tcttgagggg ttttttgctg aaacctcagg cattgaacct gcacggtacc gagctcgaat
    10021 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    10081 cgccttgcta tcgtggccgg atcttgcggc ccctcggctt gaacgaattg ttagacatta
    10141 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc
    10201 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
    10261 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
    10321 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
    10381 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
    10441 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
    10501 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
    10561 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
    10621 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
    10681 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
    10741 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
    10801 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
    10861 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
    10921 ttccctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10981 gagcggatac atatttgaat gtatttagaa aaataaacaa atagctagct cactcggtcg
    11041 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga
    11101 ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg
    11161 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt ttccggtgca
    11221 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac
    11281 ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg ttccgaccct
    11341 gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc gctttctcat
    11401 agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc
    11461 aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca
    11521 acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg gagttcgcag
    11581 aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac
    11641 tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc
    11701 cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc
    11761 aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac
    11821 cgctctagat ttcagtgcaa tttatctctt caaatgtagc
//
