LOCUS       sequence-318617-        1379 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modified lentiviral pKLV2-U6gRNA(BbsI)-PGKpuro2ABFP-W (#67974) 
            CRISPR gRNA expression vector which includes the ccdB gene flanked 
            by BbsI sites, to improve efficiency of oligonucleotide cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Goncalves E, Thomas M, Behan FM, Picco G, Pacini C, Allen F, Vinceti
            A, Sharma M, Jackson DA, Price S, Beaver CM, Dovey O, Parry-Smith D,
            Iorio F, Parts L, Yusa K, Garnett MJ
  TITLE     Minimal genome-wide human CRISPR-Cas9 library.
  JOURNAL   Genome Biol. 2021 Jan 21;22(1):40. doi: 10.1186/s13059-021-02268-4.
  PUBMED    33478580
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2021-01-21"; volume: "22(1):40. doi"; pages: " 
            10.1186/s13059-021-02268-4"
COMMENT     Sequence Label: pKLV2-U6gRNA(BbsI)-ccdB-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..1379
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(760..780)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gnnggctgat taaggagaac atgcacatga agctgtacat ggtagggcac cngtggacaa
       61 ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
      121 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag
      181 cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa
      241 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgagg acgggggcgt
      301 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
      361 cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
      421 ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc
      481 cctgaagctc gtgggcggga gccatctgat cgcaaacatc aagaccacat atagatccaa
      541 gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag
      601 aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata
      661 ctgcgacctc cctagcaaac tggggcacaa gcttaattga gcggccgctc aacctctgga
      721 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
      781 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
      841 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
      901 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
      961 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     1021 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     1081 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgcccc
     1141 tggattcggg cggggacgtc cttctgctac gtcccttcgg ccctcattca agggaacttt
     1201 ctttccgggg ctggtggcgg gtctgggggc tcttcngggt taagccttcg ccccagaaaa
     1261 aatcggaatc ccctttgggc cccccccccg ggggtccttt aaacaaatgg ctttaagggg
     1321 gngggaaatt tatccttttt aaaaaaaggg gggggggggg gtnntccccc caaagatta
//
