LOCUS       sequence-318619-        1231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modified lentiviral pKLV2-U6gRNA(BbsI)-PGKpuro2ABFP-W (#67974) 
            CRISPR gRNA expression vector which includes the ccdB gene flanked 
            by BbsI sites, to improve efficiency of oligonucleotide cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Goncalves E, Thomas M, Behan FM, Picco G, Pacini C, Allen F, Vinceti
            A, Sharma M, Jackson DA, Price S, Beaver CM, Dovey O, Parry-Smith D,
            Iorio F, Parts L, Yusa K, Garnett MJ
  TITLE     Minimal genome-wide human CRISPR-Cas9 library.
  JOURNAL   Genome Biol. 2021 Jan 21;22(1):40. doi: 10.1186/s13059-021-02268-4.
  PUBMED    33478580
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2021-01-21"; volume: "22(1):40. doi"; pages: " 
            10.1186/s13059-021-02268-4"
COMMENT     Sequence Label: pKLV2-U6gRNA(BbsI)-ccdB-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..1231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(88..107)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     promoter        complement(join(623..629,630..647,648..653))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(805..828)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cnnnggnang cncgtagnnn tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
       61 cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc
      121 cccgatatgc accaccgggt aaagtttacg ggagacttta tctgacagca gacgtgcact
      181 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac
      241 aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagt
      301 ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc
      361 cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg
      421 ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc
      481 gctgtcaact gtcactgtaa tacgctgctt catagcacac ctctttttga catacttcgg
      541 gtatacatat cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt
      601 tatctggctt ttagtaagcc ggcattatac gagccggaag cataaagtgt aaagaagacc
      661 tgtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
      721 cttgaaaaag tggcaccgag tcggtgcttt ttttggatcc aattctaccg ggtaggggag
      781 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
      841 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
      901 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
      961 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     1021 ctcgtgcaga tggacagcac cgctgaacaa tggaagcggg taggcctttg gggcagcggc
     1081 caatagcagc tttgctcctt cgctttctgg gctcaaaggc tgggaaaggg gtgggtccgg
     1141 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc ccaaaggtcc tccggaaggc
     1201 ccggcattct gcaagcttca aaagngacgt t
//
