LOCUS       sequence-318632-        2363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Untagged Kan resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 41 pCOLI_K_NoTag
FEATURES             Location/Qualifiers
     source          1..2363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(61..80)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     756..775
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        756..774
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             812..834
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(960..978)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      974..1021
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1251..2066)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1251..2066)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1974..1993
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2067..2158)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(2174..2363,1..560))
                     /direction=LEFT
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     complement(2184..2201)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctgacgagca tcacaaaaat cgacgctcaa gccagaggtg gcgaaacccg acaggactat
       61 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      121 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      181 cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      241 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      301 cggtaagaca cgacttatcg ccactggcag cagccattgg taactgattt agaggacttt
      361 gtcttgaagt tatgcacctg ttaaggctaa actgaaagaa cagattttgg tgagtgcggt
      421 cctccaaccc acttaccttg gttcaaagag ttggtagctc agcgaacctt gagaaaacca
      481 ccgttggtag cggtggtttt tctttattta tgagatgatg aatcaatcgg tctatcaagt
      541 caacgaacag ctattccgtt actctagatt tcagtgcaat ttatctcttc aaatgtagca
      601 cctgaagtca gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc
      661 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
      721 aatgagtgag ctaacttaca ttaattgcgt tgcgctaata cgactcacta tagggagacc
      781 acaacggttt ccctccggct ccggaaataa ttttgtttaa ctttaagaag gagactggat
      841 ccgaatctag agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atgtacaacg
      901 cgtgcgcgct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      961 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     1021 gctgaaacct caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc
     1081 ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgacaag
     1141 ctgacgaccg ggtctccgca agtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1201 gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac
     1261 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     1321 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     1381 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     1441 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     1501 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
     1561 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     1621 agacgaaata cgcggtcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     1681 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     1741 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     1801 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     1861 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     1921 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     1981 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa
     2041 gacgtttccc gttgaatatg gctcatactc ttcctttttc aatattattg aagcatttat
     2101 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2161 ggcatgcagc gctcttccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     2221 cggcgagcgg tgtcagctca ctcaaaagcg gtaatacggt tatccacaga atcaggggat
     2281 aaagccggaa agaacatgtg agcaaaaagc aaagcaccgg aagaagccaa cgccgcaggc
     2341 gtttttccat aggctccgcc ccc
//
