LOCUS       sequence-318636-       17690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target Per2 gene with a color-switching luciferase cassette in mouse
            genome and the transfection of the recombinant DNA will lead genomic
            recombination and make a knock-in allele in mouse ES cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17690)
  AUTHORS   Shan Y, Abel JH, Li Y, Izumo M, Cox KH, Jeong B, Yoo SH, Olson DP, 
            Doyle FJ 3rd, Takahashi JS
  TITLE     Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus 
            Reveals a Circadian Role in Network Synchrony.
  JOURNAL   Neuron. 2020 Aug 4. pii: S0896-6273(20)30529-8. doi: 
            10.1016/j.neuron.2020.07.012.
  PUBMED    32768389
REFERENCE   2  (bases 1 to 17690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Aug 4. pii: S0896-6273(20)30529-8. doi: 
            10.1016/j.neuron.2020.07.012."
COMMENT     Sequence Label: Per2:iLuc
FEATURES             Location/Qualifiers
     source          1..17690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             263..835
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          912..977
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1038..1172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1075..1094)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1129..1148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1192..1208
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7486..7519)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7522..9147
                     /codon_start=1
                     /product="red-emitting variant of click beetle luciferase"
                     /label=CBRluc
                     /note="human codon-optimized"
                     /translation="VKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVVG
                     DESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPVN
                     ESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESLP
                     NFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDPR
                     YGTQLIPGVTVLVYLPFFHAFGFHITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRSV
                     INVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLTE
                     STSAIIQTLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYVN
                     NVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKNP
                     CIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVDS
                     IPRNVTGKITRKELLKQLLEKAGG"
     RBS             8469..8477
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    9158..9619
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     CDS             complement(9633..10436)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9744..9763)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10354..10373
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(10450..10949)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10498..10517)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     10891..10914
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(10973..11006)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             11009..12634
                     /codon_start=1
                     /product="green-emitting variant of click beetle 
                     luciferase"
                     /label=CBG99luc
                     /note="human codon-optimized"
                     /translation="VKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVVG
                     DESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPVN
                     ESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESLP
                     NFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDPR
                     VGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRSV
                     INVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLTE
                     STSANIHSLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYVN
                     NVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKNP
                     CIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVDS
                     IPRNVTGKITRKELLKQLLEKAGG"
     RBS             11956..11964
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      15872..15881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        15925..16029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(16030..16098,16099..16890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(16248..16267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      17061..17649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17550..17569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaccgaa caactccgcc gcgcgcgctc cgtgtaggcc ggtgcgggcg gccccgtagc
       61 gcaagggagg gcgggaaagg aaggggcggg acacaagggc gaatctataa agggcgtcac
      121 tcagccagtt ctctcctcag aagcgccgag agcgcgaccg ggacggttgg agaagaaggt
      181 ggctcccgga agggggagag acaaactgcc gtaacctctg ccgttcagga tctgcgacct
      241 gcaggtcctc gccatggacc ctgatgatgt tgttgattct tctaaatctt ttgtgatgga
      301 aaacttttct tcgtaccacg ggactaaacc tggttatgta gattccattc aaaaaggtat
      361 acaaaagcca aaatctggta cacaaggaaa ttatgacgat gattggaaag ggttttatag
      421 taccgacaat aaatacgacg ctgcgggata ctctgtagat aatgaaaacc cgctctctgg
      481 aaaagctgga ggcgtggtca aagtgacgta tccaggactg acgaaggttc tcgcactaaa
      541 agtggataat gccgaaacta ttaagaaaga gttaggttta agtctcactg aaccgttgat
      601 ggagcaagtc ggaacggaag agtttatcaa aaggttcggt gatggtgctt cgcgtgtagt
      661 gctcagcctt cccttcgctg aggggagttc tagcgttgaa tatattaata actgggaaca
      721 ggcgaaagcg ttaagcgtag aacttgagat taattttgaa acccgtggaa aacgtggcca
      781 agatgcgatg tatgagtata tggctcaagc ctgtgcagga aatcgtgtca ggcgatctct
      841 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
      901 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
      961 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
     1021 cagatccact aggatctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1081 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1141 aactcatcaa tgtatcttat catgtctgga tcgtagttct agagcggacc ggtaaaacga
     1201 cggccagtgt taactctagg aattcgcagt gcagcccctg ccattcgctg cccctttggc
     1261 tcctgtcatg gccttcatgc tgcccagcta cccgttccca ccagcaaccc caaacctgcc
     1321 tcaggccttc ctccccagcc agcctcactt tccagcccac cccacacttg cctccgaaat
     1381 aactcctgcc tcccaggctg agttccctag tcggacctcg acgctcagac agccgtgcgc
     1441 ttgcccagtc acccctccag ccggcacagt ggccctgggc agagcctccc caccgctctt
     1501 ccagtccaga ggcagtagtc ccctacaact taacctgctt cagctagagg aggcgcctga
     1561 aggcagcact ggagccgcag ggaccctggg gaccacaggg acagcagctt ctggtctgga
     1621 ctgcacatct ggcacatctc gggatcggca gccaaaggca cctccaacag taaggcttct
     1681 ctgctgtgtc tctttctcag gcagtacaca tggtagtcag cacacaccca catgtcagag
     1741 gggctgctgg gtcgagggct tcctgccagc ctccaggcct gagttcagcc cgcagtgcct
     1801 tttctggagt gtgcacttca tagttatgag gtcctaccat gggacattat ttggagctct
     1861 atgaggagca aacgagggat catgcaaatg gagggaccaa gaataatgtg tgcctttggg
     1921 agcatttagg agcctcacat ttctcttctt tttttttttt tcctgttttt tttttctttt
     1981 agtttttcaa gacagggttt ctctgtagaa ccttggctat cttggaactc acagagatct
     2041 gcctgtgtct gcctctcaaa tgctggtatt aaaggcatgc accaccactg cccagatggg
     2101 cctcacattt ctaatgagtc ttaaaattgt ttttcctttt tcttttctct tttttctttt
     2161 cttttttctt ttctcttctt ctcttctctt ttcttttctt tttaaaattt gagacagagt
     2221 cttactctat ccctgattgt cctggaattt gctctgtaga ccaggttggc cttaaaatgc
     2281 acagagatct gtctgcctca gctctaagtg cggggattaa aagcatgccc ttgtgtggct
     2341 agtctacaaa gatgaatgga acccagcaca gggaaaaggg ccaggttcca gcctgcagtg
     2401 caggatcctg cccagtgaag tccaggctcc cggagctcac aaggatgaaa gaggacctcc
     2461 gcttttggct caggagccag tgtgcctgag agccttaagg atggtgccaa gggcttttct
     2521 ctagtaatgg tttccagaag aactacctat atagtagcag cagcccaagt cagccccgtg
     2581 cactgtgtgc cctgtgtgcc ctgtgtgccc tgtgtgccct gtgtgccctg tgtgcactgt
     2641 gtgccctgtg tgcmctgtgt gcmctgtgtg cmctgtgtgc cctgtgtgcm ctgtgtgcmc
     2701 tgtgtgcact gtgtgccctg tgtgccctgt gtgccctgtg tgccctgtgt gccctgtgtg
     2761 ccctgtgtgc cctgtgtgca ctgtgtgcac tatgtgcact gtgtgccctg tgtgcactgt
     2821 gtgccctgtg tgcactgtgt gcactgtgtg cactatgtgc actgtgtgcc ctgtgtgcac
     2881 tgtgtgcact atgtgcactg tgtgccctgt gtgcactgtg tgccctgtgt gcactgtgtg
     2941 ccctgtgtgc actatgtgca ctgtgtgcac tgtgtgccct gtgtgcactg tgtgcactgt
     3001 gtgccctgtg tgcmctgtgt gcmctgtgtg ccctgtgtgc mctgtgtgcm ctgtgtgccc
     3061 tgtgtgcmct gtgtgcactg tgtgccctgt gtgcactgtg tgcmctgtgt gccctgtgtg
     3121 cmctgtgtgc mctgtgtgcc ctgtgtgcmc tgtgtgccct gtgtgcmctg tgtgccctgt
     3181 gtgccctgtg tgcmctgtgt gccctgtgtg cactatgtgc actgtgtgcc ctgtgtgcac
     3241 tgtgtgccct gtgtgccctg tgtgcactgt gtgccctgtg tgcactgtgt gccctgtgtg
     3301 cactgtgtgc cctgtgtgtg tcagcgtaca gggctgagtg ctgccttcac aaggtgtcgg
     3361 cgctgctgtt cagaacatgg ggacgtgaga gtgggcagtg cttctcccgg agcctctctg
     3421 ccctctaaga cactaggacc ccgggtgcct cctaagccaa gcagccccac tggggctcag
     3481 agtctcagtc atgggtggct tactgccccg gaggcagagg agcatcatgg ctctttatcc
     3541 actggagtcc aagctatttg ctgtgaccgt cccaaagtca cattttattg ggtggtattc
     3601 atagggtacc agctactctc tccccataac cacaaaatct gcctgcctga cagacaggta
     3661 aaggaaaggc atgtgctcta taactgatct ttgcacaaaa gccaccctcg gcacgccctt
     3721 ggttccacct ccccttactg tattttgtat gctagactat cactgtaagc cctatggtca
     3781 gtagtgtctt tcctgtccct aaagtgcaac gagccctcag acactcagaa cagtgatgcc
     3841 atctccacgt caagtgacct gctcaacctc cttctgggcg aggacctctg ctcggccact
     3901 ggctcagccc tgtctagaag cggggcatcc gccacctcag actctctggg ctccagctcg
     3961 ctgggcttcg gcacatccca aagtggggca ggtatgtttg tgctgaggac acagctgtat
     4021 ggtagccagt gtagaagatg ctcatggaat attctatccc tggctgctct gcctggatgt
     4081 agctccttct gcccgaatca gcagcacaga gggctctagt catcagaatt gggagtccta
     4141 gctggtagcg tccaatttct gtgtcactga agaggttaca ttcatgccag cccttgcagg
     4201 gttagtctgt gggttccctg ggcattgggc acacatgtgc agacaaaact aacctctaat
     4261 ggaaggaatc acccacattt gtcctagagc ctccagggac aaggccaccc tcaaaatcct
     4321 gatgcctttt ctgccctaaa gtgagctttc cttaacctgc tcccaggcct ctctgcccag
     4381 gggcagcagc tgggcacaag tcaggccaac aaggccctct taggacaact cctctctggg
     4441 tttctaaagg tggatggatt tgtttccaga gtcaacagag agtgaaaacc tgggacaaag
     4501 gtcagggctc caagaaaggc actgcctgag ttcaggttca ccccaggcaa gagtcacctc
     4561 ctctgtgcta gcaggtatcc cgcgttcgaa tcattgagat cattaagact gaagagcaaa
     4621 ggggaagggg ggccagcaca ggctgtttct acccaaggaa ccccactctc cagagctgcc
     4681 atggctacgc attagcacat tgtgcaagag tcactctcta atgtcaaggt tataacgatc
     4741 ggcgtttcgt gtatttgaag gcagtagtga cacaagtcac accagcaaat actttggaag
     4801 cattgactct tcagagaata atcacaaagc aaaaatgatc ccagacacgg aggaaagcga
     4861 gcagttcatt aagtacgtct tgcaggaccc catctggctg ctgatggcca acacagacga
     4921 cagcatcatg atgacatacc agctgccctc ccggtaactg ttacacttcc tgggaagcct
     4981 ggagggctgg gtaggcgtgt ccaacaccaa ggtcatccat gcaggcagga taccttggaa
     5041 cactggctag acttggcaat ggagagggaa gaggcttctc cttcataacc ccactgggcc
     5101 tgcagcagcc tgcctactgc cctgaggcag ttagaaccca ggagatcaac ctcttgccag
     5161 cagcacactc ttcttttgcc atacattggt gcagtgttgc cccttgaact gtgttacatc
     5221 acccacctta ggggtaaatg ggatgtgtgt ctgtctgagt caaaaaaaaa aaaaaaaaac
     5281 aagggtcaaa gtgggtgatc agcccatgtc tgtggtaacc ctgggcctgc cagagttact
     5341 cagagcccgt cagagtatag gtcaggcgtc cctgtgaagc cagtgaggtc actatctttg
     5401 cagaacagcc ccagacaggg gctaagagaa aggaagggcg ccctgaggtt cctgaaggca
     5461 gcagacctct ggtcagtgac acgggagtaa aggctgggga gtctcagacc tctggtggga
     5521 gacagctgtg ctccaggctg gtgttgacgc aggaaggaga cagcatggtc ccagagtcta
     5581 gatggttaag tctcctaggc tgaccacaca gtgagagcca gcccagggct ccagacagca
     5641 tctgaagatg cccggctccc ctagctagtt tcatgaaaaa ctgaaattgt gacctaaaga
     5701 atatttacta gagagatgca gttactttat ctcaaggata atctactact atattgttga
     5761 tattgaaaaa aaattgatga atgaattatc cctttggcct gtgacactca cctataccta
     5821 actcttggtg aagggatctc caggcggtgt tgaaggagga ccaggagaag ctgaagctgc
     5881 tgcagaggtc ccagccccgg ttcacagagg gccagaggcg agagctccga gaggttcatc
     5941 cgtgggtcca cactgggggc ctgcctacgg ccatcgatgt gacagtaagt ttgcaggacc
     6001 ttccttctca catagcactc cgagattata cctggggcta agcatggtag cacacacctg
     6061 ccaccccagc acttgggaga ctgaggcagg aggactgcag gtctgtgtct aatctggcta
     6121 catagaagat tctgccacaa aacaagaaaa ggttctaggg acctgtctta gtcagggttt
     6181 ctattcctgc acaaacatca tgaccaagaa gcaagttggg gaggaaaggg tttattcggc
     6241 ttacacttct atactgctgt tcaccaagga agtcaggact ggaactcaag taggtcagaa
     6301 agcaggagct gatgcagagg ccatggaggg atgttcttta ctggcttgct tcccctggct
     6361 tgctcagcct tctctcttat agaaccaaga ctaccagccc agagatggtc ccacccacaa
     6421 ggggcctttc cccctcgatc acttattgag aaaatgcctt acagttggat ctcatggagg
     6481 catttcctca actgaagctc ctttctctgg gataactcca gcctgtgtca agttgacaca
     6541 aaactagcca gtacagtacc cttaggggtg atcccttcca gaggtttaag tggccggctt
     6601 gaacagcttg ctggcgtcca tggctaccct ttccaccctt ctggtttctg ttgagcaaca
     6661 taaacatttc tctgtccacc ttgttcttcc tgtcatggct cctgggcctt aaaatcccag
     6721 agccatatat gctccccagg cctctgccca tctgctggct cataggcggg gggggggggg
     6781 gggggcgctg cagtccagtg gcaacactag ccatatccat gctcttccca gctcacagga
     6841 tggaggccca ccttgccaag ctgctaacct tagggccatc tagcatgcct ctcctactcc
     6901 atctgttcac agattacagc actcaacttt actaggtaga cagccagttc ccaccacctc
     6961 caacacggcc ccctgaatgc ccaatcttag aagtcataca cttcccaagt ctaagcattc
     7021 tttatgggga aagaagtgag actctgccta ttgcaggagg agtggccttt gcaaagactt
     7081 tgtggtcatc tggccaccta cccttgtccc tcacactcac ataccctctt cacctgagca
     7141 tgtgccatga aacacttctg atggacatcc ctggaaaggg actcatgacc aggagccagc
     7201 ttcctgaggc agggagtgcc tctttggtta gcaacctcca ggtgcatggc taacattcct
     7261 ccctatcctg ctgagtggga atgacagaga agattccagc tgcttgcttc ttactcatgt
     7321 gacctgttct ctctgaaggg ctgtgtttac tgcgagagtg aggagaaagg caacatttgc
     7381 ctgccatatg aggaagacag tccttccccg ggactctgtg atacctcaga agccaaagag
     7441 gaggaaggtg aacagctgac aggccccagg atagaggccc agacgataac ttcgtatagc
     7501 atacattata cgaagttatt ggtaaagcgt gagaaaaatg tcatctatgg ccctgagcct
     7561 ctccatcctt tggaggattt gactgccggc gaaatgctgt ttcgtgctct ccgcaagcac
     7621 tctcatttgc ctcaagcctt ggtcgatgtg gtcggcgatg aatctttgag ctacaaggag
     7681 ttttttgagg caaccgtctt gctggctcag tccctccaca attgtggcta caagatgaac
     7741 gacgtcgtta gtatctgtgc tgaaaacaat acccgtttct tcattccagt catcgccgca
     7801 tggtatatcg gtatgatcgt ggctccagtc aacgagagct acattcccga cgaactgtgt
     7861 aaagtcatgg gtatctctaa gccacagatt gtcttcacca ctaagaatat tctgaacaaa
     7921 gtcctggaag tccaaagccg caccaacttt attaagcgta tcatcatctt ggacactgtg
     7981 gagaatattc acggttgcga atctttgcct aatttcatct ctcgctattc agacggcaac
     8041 atcgcaaact ttaaaccact ccacttcgac cctgtggaac aagttgcagc cattctgtgt
     8101 agcagcggta ctactggact cccaaaggga gtcatgcaga cccatcaaaa catttgcgtg
     8161 cgtctgatcc atgctctcga tccacgctac ggcactcagc tgattcctgg tgtcaccgtc
     8221 ttggtctact tgcctttctt ccatgctttc ggctttcata ttactttggg ttactttatg
     8281 gtcggtctcc gcgtgattat gttccgccgt tttgatcagg aggctttctt gaaagccatc
     8341 caagattatg aagtccgcag tgtcatcaac gtgcctagcg tgatcctgtt tttgtctaag
     8401 agcccactcg tggacaagta cgacttgtct tcactgcgtg aattgtgttg cggtgccgct
     8461 ccactggcta aggaggtcgc tgaagtggcc gccaaacgct tgaatcttcc agggattcgt
     8521 tgtggcttcg gcctcaccga atctaccagt gcgattatcc agactctcgg ggatgagttt
     8581 aagagcggct ctttgggccg tgtcactcca ctcatggctg ctaagatcgc tgatcgcgaa
     8641 actggtaagg ctttgggccc gaaccaagtg ggcgagctgt gtatcaaagg ccctatggtg
     8701 agcaagggtt atgtcaataa cgttgaagct accaaggagg ccatcgacga cgacggctgg
     8761 ttgcattctg gtgattttgg atattacgac gaagatgagc atttttacgt cgtggatcgt
     8821 tacaaggagc tgatcaaata caagggtagc caggttgctc cagctgagtt ggaggagatt
     8881 ctgttgaaaa atccatgcat tcgcgatgtc gctgtggtcg gcattcctga tctggaggcc
     8941 ggcgaactgc cttctgcttt cgttgtcaag cagcctggta aagaaattac cgccaaagaa
     9001 gtgtatgatt acctggctga acgtgtgagc catactaagt acttgcgtgg cggcgtgcgt
     9061 tttgttgact ccatccctcg taacgtaaca ggcaaaatta cccgcaagga gctgttgaaa
     9121 caattgttgg agaaggccgg cggttagccg cggcagcttc tgatggaatt agaacttggc
     9181 aaaacaatac tgagaatgaa gtgtatgtgg aacagaggct gctgatctcg ttcttcaggc
     9241 tatgaaactg acacatttgg aaaccacagt acttagaacc acaaagtggg aatcaagaga
     9301 aaaacaatga tcccacgaga gatctataga tctatagatc atgagtggga ggaatgagct
     9361 ggcccttaat ttggttttgc ttgtttaaat tatgatatcc aactatgaaa cattatcata
     9421 aagcaatagt aaagagcctt cagtaaagag caggcattta tctaatccca ccccaccccc
     9481 acccccgtag ctccaatcct tccattcaaa atgtaggtac tctgttctca cccttcttaa
     9541 caaagtatga caggaaaaac ttccatttta gtggacatct ttattgttta atagatcatc
     9601 aatttctgca gacttacagc ggatcgatcc cctcagaaga actcgtcaag aaggcgatag
     9661 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     9721 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     9781 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     9841 atattcggca agcaggcatc gccatgggtc acgacgagat catcgccgtc gggcatgcgc
     9901 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     9961 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
    10021 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
    10081 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
    10141 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
    10201 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
    10261 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
    10321 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
    10381 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatggccga tcccatattg
    10441 gctgcaggtc gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt
    10501 ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag
    10561 cccgcccctg agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa
    10621 ggagcaaagc tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt
    10681 gctgtccatc tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga
    10741 cgcgagctgc ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa
    10801 ggggccacca aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg
    10861 ccagaggcca cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga
    10921 ctgccttggg aaaagcgcct cccctacccg gtagaattca ctctagagtt agataacttc
    10981 gtatagcata cattatacga agttattggt gaagcgtgag aaaaatgtca tctatggccc
    11041 tgagcctctc catcctttgg aggatttgac tgccggcgaa atgctgtttc gtgctctccg
    11101 caagcactct catttgcctc aagccttggt cgatgtggtc ggcgatgaat ctttgagcta
    11161 caaggagttt tttgaggcaa ccgtcttgct ggctcagtcc ctccacaatt gtggctacaa
    11221 gatgaacgac gtcgttagta tctgtgctga aaacaatacc cgtttcttca ttccagtcat
    11281 cgccgcatgg tatatcggta tgatcgtggc tccagtcaac gagagctaca ttcccgacga
    11341 actgtgtaaa gtcatgggta tctctaagcc acagattgtc ttcaccacta agaatattct
    11401 gaacaaagtc ctggaagtcc aaagccgcac caactttatt aagcgtatca tcatcttgga
    11461 cactgtggag aatattcacg gttgcgaatc tttgcctaat ttcatctctc gctattcaga
    11521 cggcaacatc gcaaacttta aaccactcca cttcgaccct gtggaacaag ttgcagccat
    11581 tctgtgtagc agcggtacta ctggactccc aaagggagtc atgcagaccc atcaaaacat
    11641 ttgcgtgcgt ctgatccatg ctctcgatcc acgcgtgggc actcagctga ttcctggtgt
    11701 caccgtcttg gtctacttgc ctttcttcca tgctttcggc tttagcatta ctttgggtta
    11761 ctttatggtc ggtctccgcg tgattatgtt ccgccgtttt gatcaggagg ctttcttgaa
    11821 agccatccaa gattatgaag tccgcagtgt catcaacgtg cctagcgtga tcctgttttt
    11881 gtctaagagc ccactcgtgg acaagtacga cttgtcttca ctgcgtgaat tgtgttgcgg
    11941 tgccgctcca ctggctaagg aggtcgctga agtggccgcc aaacgcttga atcttccagg
    12001 gattcgttgt ggcttcggcc tcaccgaatc taccagcgct aacattcact ctctcgggga
    12061 tgagtttaag agcggctctt tgggccgtgt cactccactc atggctgcta agatcgctga
    12121 tcgcgaaact ggtaaggctt tgggcccgaa ccaagtgggc gagctgtgta tcaaaggccc
    12181 tatggtgagc aagggttatg tcaataacgt tgaagctacc aaggaggcca tcgacgacga
    12241 cggctggttg cattctggtg attttggata ttacgacgaa gatgagcatt tttacgtcgt
    12301 ggatcgttac aaggagctga tcaaatacaa gggtagccag gttgctccag ctgagttgga
    12361 ggagattctg ttgaaaaatc catgcattcg cgatgtcgct gtggtcggca ttcctgatct
    12421 ggaggccggc gaactgcctt ctgctttcgt tgtcaagcag cctggtaaag aaattaccgc
    12481 caaagaagtg tatgattacc tggctgaacg tgtgagccat actaagtact tgcgtggcgg
    12541 cgtgcgtttt gttgactcca tccctcgtaa cgtaacaggc aaaattaccc gcaaggagct
    12601 gttgaaacaa ttgttggaga aggccggcgg ttaggatatc aagcttgggg aagatcttgt
    12661 ggtttctaat cacatggacc catacctaca ctgctttttt ttgttttagg aaaaacaaaa
    12721 aacaaaaaca ccatagtttt ctggcggtgg aacaaaactg aggggaggtt taggaggaaa
    12781 tccatttttg tattaaaata gaaatacgga atttggggga tggggtgaga ttcgtcattg
    12841 aacttgagac tgaggtggtc tgtgttgtca tggaggctgc ctcatggtcc tcaggagtgt
    12901 cttgacctcc atgaaacctc tttccagtgt gccaatgtcc tctggcccct gtggattgtt
    12961 ctgaaacata acaccaggat gtggcaggta acagggaagc cacaagaggc tatccaccaa
    13021 gggcccagct ttctggaact ttctcacagt gtgattgtat ctcccaagca gagagaccat
    13081 ctctcctgac atcctctcag tgtgttccct tacgtggttt ggagcatggt gtagcagctt
    13141 tggcttcagg tcctgcctgt ggtggtcaac attccagtct gacatggctt ctgttcgtca
    13201 acaaagttga aatgcctgct ctggactagt ggagctcagt ggcttctgca aacgatgccc
    13261 accatcagac tagcaccccc acactgtaca tttctctgct gttcttgtat cctttttaga
    13321 ccattgtggc cagtgtgcag agagagctgt ggcatcatca gccatgttgc cgtgtctgca
    13381 tggtggcctc tgcaagccag gctttgttgc tgtagaggac accgtcacgt gtttgttctt
    13441 tggttggact ctctcagaca ttagctccca gcagaaagca gccactgagc acggaggaga
    13501 gggcacccac actgctgccc tgagttctcc agtttgcagg gagctcagcc tcccactcta
    13561 tatgtatata ctcctagcta ctgtggcttc gggtctctgt cacatctatc tgtgctgctg
    13621 gtcctcagat cactggaacc tgtggagaga aggggacctc tctgcccagc tctacaaaac
    13681 tttatgctgc atcagacatc caaagattgt tccgacatgc ttgcgggtga cccctggtgg
    13741 aacgagacat caccagtgag gaatcattgg acttaaaagt agacaaagcc tggagcagag
    13801 gaagctgttt cctgagtctg aagtggctac tggggacatg tcctgctgta gttggttttc
    13861 atggtaaagc catctgaggc ctgaatatta cccctatttt tcataaacac aagaactcta
    13921 ttttttttat taaagcaaca ccacctttca cagtgatcag gtagtagcca tgttttaaag
    13981 gaaattcaat gttacagaca gctgcctctc tgaccagtct gatcctaagg gtagatagaa
    14041 gatggtctaa gcctacgctt gttacttaaa cacaaaactg ccaaaacctt ctctcttctc
    14101 tcttgaatgt ttaccatcag cgttatttta tgattattta atatatagtc cttgattgtt
    14161 aactgctaag aagttgactt cctaggataa ttttgtgaat ctgtttacaa gatgccaagc
    14221 atccagccct gttttcttta gaatgtgtgc ttacacgggt gtcctaagac attctctatt
    14281 ttaaactgag ccttcttttt aatgtaaata agctctcaga gtttgtgcga tgatgattcg
    14341 tgagccttgc cggacaagag gtttgttcat gcgcaaacca aacgtacctt cacccagtgc
    14401 aatatatttg tgtgactgct tgtgtctttt tatgactttt ttgcctttta gaaaattgtt
    14461 aaataaagca agtatatttt tattttcaac tgtatgcttt gtttgatgtg atggtgggga
    14521 tttaacccag gacctcatgc aaattaagcg tgtgttctaa cattgaccta taccctagtg
    14581 ctgtatatat ttttaacata acatagacca ataagccaaa aagaatgaca ttgtcaaaaa
    14641 taataaaaga aaaaaaccca cacaaacaac aacaacaaca aaccttttta agaacttcaa
    14701 aatggtacat taggaaatct ccagttccag aagccagcag ctcttacttc ccttgcactg
    14761 tgggctttgc tggccgatct cctgtctgtg tatctctcac atgctcagtc tctctgggcc
    14821 attcaaaccc tgtagggatt tcctatgatg cagatctagc caggcatccc caggtccctt
    14881 tctcttggtg tctctttaca tgcacacagt ttagcagatt cactaggctg cccctcagct
    14941 tagcatgacc cccgcccagc acgcactctc ccaataatcc cgcctttggc tgtttgcctg
    15001 tgtgttggtc tcagggctga ttgcagagaa atcagacaag taccatgcta gaaaggacct
    15061 atataccgtt aggatcagta tataggcatc actggggaca caggcctcct acctgaccct
    15121 tcctgattga cagccttgtg agagctactc tgaagagacc tgcttccctt tcctctctcc
    15181 aaaccagggt cccagggacc ggagcagagg ggcaacatga cactggacct cactcacggt
    15241 ctgcttctgg gtgctctgaa cctgccttct gactgcaagt actggtgcag tctgacacaa
    15301 agttctcgtg gcaggtagga ctgcttcact gttcctttgg aggactctta attgaacttt
    15361 acggccagaa ggactgcccc tctcctttct cttcaaacca aaaggaaatt cttggggtct
    15421 cctgggcaga ttgcccacaa agcccttgga agtctgcagg cctacagctg cctttgtccc
    15481 tctggggatt tacagcctgt taatttctgc tgctaactag tcaccctgtg gccatcatgg
    15541 ccttgcctag cctctggggt tctctgttcc tgcctggggg gggggggggg gattaaaagg
    15601 ccctactact aaggaaatct gcatgcaggc aaactttgaa ggtagggtat ggttcagggg
    15661 acaaatggca ggaactagta gtttaactga gggaggtgaa caggagaagg gtttcctgag
    15721 cctgagatgg ggtgagtagt cacgctgctg ggaccaatgg agtctaagcc acttggctaa
    15781 tggagtcact aaaataaaat aaattgatgc ctgcttgtac tttcccttcc tcaaaggtct
    15841 taagcttcct agacccgggg gggtcgacgc ggccgccatg gtcatagctg tttgacgtca
    15901 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    15961 tcaaatatgt atccgctcac gagacaataa ccctgataaa tgcttcaata atattgaaaa
    16021 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    16081 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    16141 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    16201 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    16261 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    16321 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    16381 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    16441 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    16501 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    16561 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    16621 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    16681 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    16741 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    16801 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    16861 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    16921 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    16981 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    17041 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    17101 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    17161 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    17221 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    17281 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    17341 agacgatagt taccggataa ggcgcagcgg tcgtgctgaa cggggggttc gtgcacacag
    17401 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    17461 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    17521 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    17581 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    17641 ctatggaaaa acgccagcaa cgcggccttt ttacggttct ggccttttgc
//
