LOCUS       sequence-318640-        9030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-cassette for SapTrap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1816
FEATURES             Location/Qualifiers
     source          1..9030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(41..629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(121..140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(776..1585)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1499..1518
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1577..1596
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1698..1714
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1698..1714
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1719..1738
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1719..1737
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1767..1856)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     protein_bind    complement(1878..1911)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     protein_bind    2264..2288
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2289..3314)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2289..3314)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(3317..3341)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      5259..5582
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(7399..7428)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(7438..7471)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(8456..8473)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8456..8472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8465..8487)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      8536..8622
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8714..8741
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(8900..8917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
       61 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      121 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      181 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      241 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      301 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      361 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      421 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      481 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      661 acgctcagtg gaacgacgcg taactcacgt taagggattt tggtcatgag cttgcgccgt
      721 cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga
      781 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
      841 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
      901 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
      961 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     1021 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     1081 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     1141 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     1201 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     1261 ctaatacctg gaatgctgtt tttccgggga tcgcagtggt gagtaaccat gcatcatcag
     1321 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     1381 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     1441 ctggcgcatc gggcttccca tacaagcgat agattgtcgc acctgattgc ccgacattat
     1501 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg
     1561 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1621 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     1681 acacgggcca gagctgccag gaaacagcta tgaccatgta atacgactca ctatagggga
     1741 tatcagctgg atggcgctct tcgcgtcaag tcttcctcgc tgatcaactt ctgctcgagg
     1801 tcctcctcgg agatgagctt ttgctcaaga tcctcttcag aaataagttt ttgttcagat
     1861 ccggctccac cctgaaaata acttcgtata gtatacatta tacgaagtta ttaaaaattg
     1921 cggtcataaa ctgaaacgta acatatgata aggtattttg tgtgcggttt tttctatgat
     1981 gtttcaaaaa aatttatcag aagtaaaaaa cataagaagt gtacacagaa cattgtgtag
     2041 attttgggat agaattatta ttaatggggg gagggagcac aatttttttc gactttttat
     2101 tacgaattaa ttctattttt gaatctacac aatttcattg ttagaggtga cttaaaagaa
     2161 gctaaaaaac aaagaaatta agagaagttt tttgataata acaaaaatag ggggtgggag
     2221 cacagggaga aagagcatgt agggatgttg aagagtaatt ggacccactt tgtacaagaa
     2281 agctgggtct attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
     2341 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
     2401 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
     2461 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
     2521 tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
     2581 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
     2641 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
     2701 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
     2761 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
     2821 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
     2881 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
     2941 gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt
     3001 tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc
     3061 tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
     3121 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
     3181 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
     3241 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
     3301 tcaggctttt tcatggagcc tgcttttttg tacaaacttg cggcaccgcc tgacatatta
     3361 ccttaaaatt caaaaattaa tttcagatca gtcataacca agaaaacaaa ataaacgtta
     3421 ggacagaaaa ttcaaattaa ttaaaaatta tgctgaataa gaaaattaca tgcaaagcct
     3481 tattttcctc atagaaacag ttgaataaca tattttttaa ttgaaaacat agcaaaattc
     3541 gtggtaaaat ttgtcaaaac cttggaaaaa atcgaaaaaa gaacatttcc cgatgcattt
     3601 tcctatttct cgttgccgac aacgtataaa cgttgaatac tcagtcaacg aatttggctg
     3661 ctggcttgga ctctcgcacg gaagagacgc gtgtgtagat ttacgacgaa agcaaaaatg
     3721 atcaccaaaa acggaacgtt gagctggacg gaaatagtgg taaagtgaca tgattatagt
     3781 ttgaagattt ctaatttcac aattagagca aatgttgttc ggtatttatt ttcaacggta
     3841 tttatactat tttccacctt tttctagaac attcgagctg cttgttgcaa aaggagggcg
     3901 actcacattc ggtacatgga aaagtagtgt acacaataaa gagacccaga tacattttcc
     3961 gtctgcgtct ctttgcaccc accgggagta ttttcaaacg aatgcatcta ggaccttcta
     4021 gaacattctg taaggctgca gaatgcgggt atataaggaa agcgggctca gaggaagcca
     4081 acacgctttg ttctagtgca tctaaaaaac ttcgaaaata tgtcaaatct tctcactgtt
     4141 catcaaaatc ttccagcact tccagttgac gcgacaagtg atgaagttcg caagaacctc
     4201 atggatatgt tccgtgacag acaagccttc tccgagcaca cctggaagat gcttctctca
     4261 gtgtgccgtt cttgggctgc ctggtgcaag cttaacaacc gcaagtggtt cccagccgag
     4321 ccagaagacg ttcgtgacta cttgctctac ctccaagctc gtggattggc ggtgaagacg
     4381 atccaacaac atctcggaca gctcaatatg ctccatcgcc gatctggact tccacgccca
     4441 tcagattcta atgccgtatc tctcgtaatg agacgtatcc gcaaggaaaa tgttgatgct
     4501 ggagagagag ccaagcaagc cctcgccttc gaaaggtaag tttaaatatt aatgtattca
     4561 atcctaagac ttgacgaaat tttcagaacc gacttcgatc aggtccgttc tcttatggaa
     4621 aattccgatc gttgccagga catccgaaac ctcgccttcc tcggaattgc ttacaacaca
     4681 cttctccgta tcgccgaaat tgcccgcatt cgcgtcaagg atattagtcg cacagacgga
     4741 ggaagaatgc tcatccacat cggacgtaca aaaacgctgg tctcgactgc cggagtcgaa
     4801 aaagcgctct cccttggagt taccaagctc gttgagcgct ggatttcggt ttccggagtc
     4861 gccgatgatc caaacaacta cctgttctgt cgtgttcgca agaacggagt tgctgccccg
     4921 agtgcaacat cacaactctc gactcgagct cttgaaggta agtttaaaca acatattagc
     4981 taagaataag ctgaaaataa attttcagga atcttcgaag ctacccaccg tctcatctac
     5041 ggagccaagg atgattccgg acaacgttac ttggcttggt ctggacattc cgcccgtgtc
     5101 ggagctgccc gtgacatggc tcgcgctgga gtttccatcc cagagattat gcaagccgga
     5161 ggttggacca acgtcaacat tgtgatgaac tatattagaa acttggattc tgaaactggc
     5221 gccatggttc gtcttctcga agacggtgat taagataaat gcaaaatcct ttcaagcatt
     5281 cccttcttct ctatcactct tctttctttt tgtcaaaaaa ttctctcgct aatttatttg
     5341 cttttttaat gttattattt tatgactttt tatagtcact gaaaagtttg catctgagtg
     5401 aagtgaatgc tatcaaaatg tgattctgtc tgatgtactt tcacaatctc tcttcaattc
     5461 cattttgaag tgctttaaac ccgaaaggtt gagaaaaatg cgagcgctca aatatttgta
     5521 ttgtgttcgt tgagtgaccc aacaaaaaga ggaaacttta ttgtgccgcc aagaaaaaag
     5581 tctcactgaa tctcaaatat tttattagaa aacaccaaca atttagatat ttctgtatcc
     5641 acggttggcg ttgacagcct ctgatggtgg agacttgtct gggcactggc agtactcggc
     5701 atcctttcct ggtcttccgt ctggtccagt ctctccctgg gttccgtctg gtccttgacg
     5761 tccttgcttt ccagatggtc cttctggtcc aacggttcct ggctctcctg gtggtccgtg
     5821 aagaccgttc tgtccagctg gtcctgttgg tccttgatct cctcttggtc cctttggtcc
     5881 tggtcttccg attggatgct cggcgtcgcg tcccttctct cctggtggtc ctggctctcc
     5941 gatctttccg atttgaccgg atgatccttg ctctcctgga tgtcctggtt gtccatctct
     6001 tcctgggttt ccattttgtc ctcttggtcc tggaagtcca cgtggtcctg gtcttccaag
     6061 agctcctggt ggtccaactg gtccttgtgg gcaagtgaag catccgacag actcgcgttg
     6121 tggagcgata tcatcagctc caactcctgg aacaccgtca agtcctggaa ctccgtcgag
     6181 tcctggaact cctggaactc cctttggtcc ggatggtcca gctgggcact tgttatcagc
     6241 attgcagttg caggttggtc catttccatt ggctccattt ggagtctttg gctggttgaa
     6301 gactggtgga actgctggtg gttgacctgg tggagcagat ggggatccat cagcatgtgg
     6361 agttggggta gcgttggtct cttcatattg acaacggaca cgcttgctag atcttccgat
     6421 gacaactatg tccttccaca atccattggt agattgctag aaaattaaaa attatttaaa
     6481 attcaaaata taatttaact cactctgaac acattcatct cggtatcaag cttctctctg
     6541 aatccatcaa cctccgagta gatggttgac atggtcatga agcataccat aagagtggcg
     6601 acagtgaccg aagccgtcac ataacacgca agttttacag acatctctga aattatcaaa
     6661 tttaggattt caaaataatt cggccaaaga aatgtaactc acctagtctt cgagaagcag
     6721 agacttgtcg atttcctacc ggattcgtct ccttttatta ggaaatcttt ccgcaagttt
     6781 tcaagttttt tttgtttttt ttttacatta ccgacgagac agtgtgcgag acgcagagac
     6841 gataaaagac tggataagaa cgggagataa ggagtacgga tgtaggtgag gaatgtggcc
     6901 gaaaggacag atgggcttta ttcgggaaga ttggttggga aatgaaccag agaagaatga
     6961 ctgaataaag ttgttgaaac ttggtgagaa tggaagatag acaatcgagg gcagctagtt
     7021 tccttcctcc tctatatatt tttatgtttt cttttaaacc ttcctccata tttctgaatt
     7081 gaatgttgtt gcatattgtg tgtccttatt tcatttccat catccttttg tgtgtttccc
     7141 ccttccacca attcccccca cgtcctaatg aaatgaaagc gagaagataa ataaacttgc
     7201 acattgtgtt cagatgagag gagcggaata cgaattggga gacggaaaga gaaaaaaaaa
     7261 tgtttagatt tggattgaat taatttttta gaaagcaaga aaaaaaacac aaagagaata
     7321 aataaataat aggaaaaaaa acaaatattc agtatttaaa atttgaaaat tcaacgacgt
     7381 tggcgtcgat catccttaga ggtcctcctc ggagataagc ttttgctctc cctgaaaata
     7441 acttcgtata gtatacatta tacgaagtta ttttaaactt accttgtaca gctcgtccat
     7501 tcctccggtc gaatgacgtc cctcggatcg ttcgtattgt tctacaactg tgtagtcttc
     7561 attatgggaa gtaatatcaa gcttgcggtc aacgttatat gctcctggca tttgaactgg
     7621 cttctttgcc ttgtatgtgg tcttgaaatc cgccaggtaa cgtcctgaaa agtaccatta
     7681 gtgtatataa gacgaaaccg tttcatcaaa cttacctccg tccttgaggc gtaacgccat
     7741 tttgatgtct cccttgagaa ctccatcttc tgggtacaat cgttcggtgg aagcctccca
     7801 tcccatcgtc ttcttctgca taactggtcc atctggtggg aaattggtac cacggagctt
     7861 gaccttgtag atcaaagtgc catcctgaaa aaatgatgtc aaacaaccat tgttaaaagc
     7921 ggagtaaaaa cttacctcga gagaagtgtc ctgagttact gtaactgctc caccatcctc
     7981 gaagttcatc acacgctccc acttgaagcc ttctgggaaa gattgcttgt agtaatctgg
     8041 aatgtcggct gggtgtttga tgaaagcacg ggagccgtac atgaattgtg gggagagaat
     8101 atcccatgag aatggaagtg gtcctgaaaa aacgatgacc attgcgaatt gaaattggat
     8161 tatcacaaac ttacctccct tggtgacctt cagcttggcg gtctgagttc cctcgtacgg
     8221 gcggccctcc ccttctcctt cgatttcgaa ctcgtgacca ttcatgcttc cttccatgtg
     8281 aaccttgaag cgcatgaact ccttgatcac agcttctcct ttggagacca tggatccgcc
     8341 tcctcctgaa ccaccgccgc cacttccgcc acctccactg cctccgccac ccgaacctcc
     8401 tcctcccgat ccaccgccac ccgccgaaga gcattatttg gggcccgagc ttaagactgg
     8461 ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc tagcgttaac gcgagagtag
     8521 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt
     8581 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     8641 aacgttgtga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     8701 catcaaacta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     8761 cctggctagc ggtacgcgta ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8821 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8881 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8941 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9001 caggaaagaa catgtgagca aaaggccagc
//
