LOCUS       sequence-318641-       13128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating transgenic mice expressing ERK biosensor and RGECO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13128)
  AUTHORS   Imanishi A, Ichise H, Fan C, Nakagawa Y, Kuwahara K, Sumiyama K, 
            Matsuda M, Terai K
  TITLE     Visualization of Spatially Controlled Vasospasm by Sympathetic 
            Nerve-Mediated ROCK Activation.
  JOURNAL   Am J Pathol. 2020 Oct 15. pii: S0002-9440(20)30459-4. doi: 
            10.1016/j.ajpath.2020.09.012.
  PUBMED    33069718
REFERENCE   2  (bases 1 to 13128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Pathol. 2020 Oct 15. pii: S0002-9440(20)30459-4. doi: 
            10.1016/j.ajpath.2020.09.012."
COMMENT     Sequence Label: pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-...
FEATURES             Location/Qualifiers
     source          1..13128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1746..1779)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1783..2448
                     /codon_start=1
                     /product="dimeric Keima-Red fluorescent protein"
                     /label=dKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPLFQYGSIPFTKYPEDIPDYVKQSFPEGYTWERTMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGTNFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGYHYIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     CDS             3163..3189
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    complement(3200..3233)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3249..3959
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELY"
     CDS             4134..4481
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     primer_bind     complement(4596..4617)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4857..4876)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             6174..6194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6201..6257
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6273..6329
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     7025..7044
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7068..7511
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     misc_feature    7535..8123
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7588..7608)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8006..8017)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    8188..8322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8212..8231)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8266..8285
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8456..8618,8619..8630)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8660..8679)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8661..8679)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8689..8706)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8689..8705)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8698..8720)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8847..9301
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8933..8952)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9143..9164
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9327..9431
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9432..9500,9501..10292)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9650..9669)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10463..11051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10952..10971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11205..11222
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11339..11360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11375..11380,11381..11398,11399..11405)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     11437..11453
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11437..11453
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11472..11492
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11474..11492
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(11549..11560,11561..11748)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc atcattttgg
     1741 caaagataac ttcgtatagc atacattata cgaagttata ccatggtgag tgtgatcgct
     1801 aaacaaatga cctacaaggt ttatatgtca ggcacggtca atggacacta ctttgaggtc
     1861 gaaggcgatg gaaaaggaaa gccttacgag ggagagcaga cagtaaagct cactgtcacc
     1921 aagggtggac ctctgccatt tgcttgggat atattatcac cactgtttca gtacggaagc
     1981 ataccattca ccaagtaccc tgaagacatc cctgattatg taaagcagtc attccctgag
     2041 ggatatacat gggagaggac catgaacttt gaagatggtg cagtgtgtac tgtcagcaat
     2101 gattccagca tccaaggcaa ctgtttcatc tacaatgtca aaatctctgg tacgaacttt
     2161 cctcccaatg gacctgttat gcagaagaag acacagggct gggaacccag cactgagcgt
     2221 ctctttgcac gagatggaat gctgatagga aacgattata tggctctgaa gttggaagga
     2281 ggtggtcact atttgtgtga atttaaatct acttacaagg caaagaagcc tgtgaggatg
     2341 ccagggtatc actatattga ccgcaaactg gatgtaacca gtcacaacag ggattacaca
     2401 tctgttgagc agtgtgaaat agccattgca cgccactctt tgctcggtgg cggcagcggc
     2461 ggcggtaccg gcggcagcgg cggcatggtc tccgtcattg ccaagcaaat gacctacaag
     2521 gtttatatgt caggcacggt caatggacac tactttgagg tcgaaggcga tggaaaagga
     2581 aagccttacg agggagagca gacagtaaag ctcactgtca ccaagggtgg acctctgcca
     2641 tttgcttggg atatattatc accactgttt cagtacggaa gcataccatt caccaagtac
     2701 cctgaagaca tccctgatta tgtaaagcag tcattccctg agggatatac atgggagagg
     2761 accatgaact ttgaagatgg tgcagtgtgt actgtcagca atgattccag catccaaggc
     2821 aactgtttca tctacaatgt caaaatctct ggtacgaact ttcctcccaa tggacctgtt
     2881 atgcagaaga agacacaggg ctgggaaccc agcactgagc gtctctttgc acgagatgga
     2941 atgctgatag gaaacgatta tatggctctg aagttggaag gaggtggtca ctatttgtgt
     3001 gaatttaaat ctacttacaa ggcaaagaag cctgtgagga tgccagggta tcactatatt
     3061 gaccgcaaac tggatgtaac cagtcacaac agggattaca catctgttga gcagtgtgaa
     3121 atagccattg caaggcatag cctgttgggc tctagactgc agctgcctcc tctggaacgc
     3181 ctgactctgg attaagtaaa taacttcgta tagcatacat tatacgaagt tatttcgaag
     3241 cccttagcat gtctaaaggt gaagaattat tcactggtgt tgtcccaatt ttggttgaat
     3301 tagatggtga tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta
     3361 cgtacggtaa attgacctta aaattactct gtactactgg taaattgcca gttccatggc
     3421 caaccttagt cactacttta ggttatggtg ttcaatgttt tgctagatac ccagatcata
     3481 tgaaacaaca tgactttttc aagtctgcca tgccagaagg ttatgttcaa gaaagaacta
     3541 tttttttcaa agatgacggt aactacaaga ccagagctga agtcaagttt gaaggtgata
     3601 ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa agaagatggt aacattttag
     3661 gtcacaaatt ggaatacaac tataactctc acaatgttta catcactgct gacaaacaaa
     3721 agaatggtat caaagctaac ttcaaaatta gacacaacat tgaagatggt ggtgttcaat
     3781 tagctgacca ttatcaacaa aatactccaa ttggtgatgg tccagtcttg ttaccagaca
     3841 accattactt atcctatcaa tctgccttat tcaaagatcc aaacgaaaag agagaccaca
     3901 tggtcttgtt agaatttttg actgctgctg gtattaccga gggtatgaat gaattgtacc
     3961 tcgagatggc ggacgaggag aagctgccgc ccggctggga gaagcgcatg agccgcagct
     4021 caggccgagt gtactacttc aaccacatca ctaacgccag ccagtgggag cggcccagcg
     4081 gcaacagcag cagtggtggc aaaaacgggc agggggagcc tgccaggggt accagtgctg
     4141 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggttccggca
     4201 gtgctggtgg tagtgctggt ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg
     4261 gtggtagtgc tggtggtagt gctggtggtt ccggcagtgc tggtggtagt gctggtggta
     4321 gtaccagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg
     4381 gtggttccgg cagtgctggt ggtagtgctg gtggtagtac cagtgctggt ggtagtgctg
     4441 gtggtagtgc tggtggtagt gctggtggta gtgctggtgg ttccggacca gatgtcccta
     4501 gaactccagt ggataaagca aagctgtcat tccaatttcc gggcggccgc atggtgagca
     4561 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtca
     4621 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4681 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4741 ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     4801 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4861 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4921 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4981 acaactactt cagcgggaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     5041 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     5101 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5161 cccagtccgc cttaagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5221 tcttgaccgc cgccgggggt accaaggtgt acgaccccga gcagaggaag aggatgatca
     5281 ccggccccca gtggtgggcc aggtgcaagc agatgaacgt gctggacagc ttcatcaact
     5341 actacgacag cgagaagcac gccgagaacg ccgtgatctt cctgcacggc aacgccacta
     5401 gcagctacct gtggaggcac gtggtgcccc acatcgagcc cgtggccagg tgcatcatcc
     5461 ccgatctgat cggcatgggc aagagcggca agagcagcaa cggcagctac aggctgctgg
     5521 accactacaa gtacctgacc gcctggttcg agcttctgaa cctgcccaag aagatcatct
     5581 tcgtgggcca cgactggggc gccgccctgg ccttccacta cgcctacgag caccaggaca
     5641 ggatcaaggc catcgtgcac atggagagcg tggtggacgt gatcgagagc tgggacgagt
     5701 ggccagacat cgaggaggac atcgccctga tcaagagcga ggagggcgag aagatggtgc
     5761 tggagaacaa cttcttcgtg gagaccgtgc tgcccagcaa gatcatgaga aagctggagc
     5821 ccgaggagtt cgccgcctac ctggagccct tcaaggagaa gggcgaggtg agaagaccca
     5881 ccctgagctg gcccagagag atccccctgg tgaagggcgg caagcccgac gtggtgcaga
     5941 tcgtgagaaa ctacaacgcc tacctgagag ccagcgacga cctgcccaag ctgttcatcg
     6001 agggcgaccc cggcttcttc agcaacgcca tcgtggaggg cgccaagaag ttccccaaca
     6061 ccgagttcgt gaaggtgaag ggcctgcact tcctccagga ggacgccccc gacgagatgg
     6121 gcaagtacat caagagcttc gtggagagag tgctgaagaa cgagcagtct agacctaaga
     6181 agaagcgtaa ggtggctagc gcgacgaatt ttagtctact gaaacaagcg ggagacgtgg
     6241 aggaaaaccc tggacctatg gtcgactcat cacgtcgtaa gtggaataag gcaggtcacg
     6301 cagtcagagc tataggtcgg ctgagctcac ccgtggtttc cgagcggatg taccccgagg
     6361 acggcgccct gaagagcgag atcaagaagg ggctgaggct gaaggacggc ggccactacg
     6421 ccgccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     6481 tcgtcgacat caagttggac atcgtgtccc acaacgagga ctacaccatc gtggaacagt
     6541 gcgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggaggta
     6601 caggcgggag tctggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     6661 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     6721 agggcgaggg ccgcccctac gaggcctttc agaccgctaa gctgaaggtg accaagggtg
     6781 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     6841 acattaagca cccagccgac atccccgact acttcaagct gtccttcccc gagggcttca
     6901 ggtgggagcg cgtgatgaac ttcgaggacg gcggcattat tcacgttaac caggactcct
     6961 ccctgcagga cggcgtattc atctacaagg tgaagctgcg cggcaccaac ttcccccccg
     7021 acggccccgt aatgcagaag aagaccatgg gctgggaggc tacgcgtgac caactgactg
     7081 aagagcagat cgcagaattt aaagaggctt tctccctatt tgacaaggac ggggatggga
     7141 cgataacaac caaggagctg gggacggtga tgcggtctct ggggcagaac cccacagaag
     7201 cagagctgca ggacatgatc aatgaagtag atgccgacgg tgacggcaca ttcgacttcc
     7261 ctgagttcct gacgatgatg gcaagaaaaa tgaatgacac agacagtgaa gaggaaatta
     7321 gagaagcgtt ccgcgtgttt gataaggacg gcaatggcta catcggcgca gcagagcttc
     7381 gccacgtgat gacagacctt ggagagaagt taacagatga ggaggttgat gaaatgatca
     7441 gggtagcaga catcgatggg gatggtcagg taaactacga agagtttgta caaatgatga
     7501 cagcgaagta atcgcgagtc gacggatcct cgataatcaa cctctggatt acaaaatttg
     7561 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7621 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7681 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7741 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7801 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7861 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7921 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     7981 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8041 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8101 ctccctttgg gccgcctccc cgcatctcga caaaggatcc aatggaggcc gccaccgcgg
     8161 tggagctcga attaattcat cgatgatgat ccagacatga taagatacat tgatgagttt
     8221 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     8281 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     8341 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     8401 tacaaatgtg gtatggctga ttatgatcct ctagatcaga tcggcgcgcc gatctttctt
     8461 gcttttactt ttacttcctt aatactcaag tacaatttta atggagtact tttttacttt
     8521 tactcaagta agattctagc cagatacttt tacttttaat tgagtaaaat tttccctaag
     8581 tacttgtact ttcacttgag taaaattttt gagtactttt tacacctctg tcaagaacca
     8641 tatgccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     8701 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     8761 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     8821 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     8881 tgtggtggtt acgcgcagcg tgaccaccac acccgccgcg ccctagcgcc cgctcctttc
     8941 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9001 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9061 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9121 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9181 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9241 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     9301 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9361 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9421 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9481 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9541 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9601 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9661 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9721 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     9781 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9841 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9901 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9961 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10021 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10081 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10141 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10201 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10261 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10321 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10381 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10441 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10501 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10561 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10621 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10681 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10741 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10801 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10861 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10921 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    10981 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11041 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11101 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11161 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11221 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11281 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11341 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    11401 tgttgtgtgg aattgtgatc ggataacaat ttcacacagg aaacagctat gaccatgatt
    11461 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc ggatctgctg
    11521 ggcttgctga aggtaggggg tcaagaacca gaggtgtaaa gtacttgagt aattttactt
    11581 gattactgta cttaagtatt atttttgggg atttttactt tacttgagta caattaaaaa
    11641 tcaatacttt tacttttact taattacatt tttttagaaa aaaaagtact ttttactcct
    11701 tacaatttta tttacagtca aaaagtactt attttttgga gatcacttgg gccctctaga
    11761 tgttaattaa cggctggccc aggccatcgg cattccggag cccagggtcc agatttggtt
    11821 tcagaatgag aggtcacgcc agctgaggca gcaccggcgg gaatctcggc cctggcccgg
    11881 gagacgcggc ccgccagaag gccggcgaaa gcggaccgcc gtcaccggat cccagaccgc
    11941 cctgctcctc cgagcctttg agaaggatcg ctttccaggc atcgccgccc gggaggagct
    12001 ggccagagag acgggcctcc cggagtccag gattcagatc tggtttcaga atcgaagggc
    12061 caggcacccg ggacagggtg gcagggcgcc cgcgcaggca ggcggcctgt gcagcgcggc
    12121 ccccggcggg ggtcaccctg ctccctcgtg ggtcgccttc gcccacaccg gcgcgtgggg
    12181 aacggggctt cccgcacccc acgtgccctg cgcgcctggg gctctcccac agggggcttt
    12241 cgtgagccag gcagcgaggg ccgcccccgc gctgcagccc agccaggccg cgccggcaga
    12301 ggggatctcc caacctgccc cggcgcgcgg ggatttcgcc tacgccgccc cggctcctcc
    12361 ggacggggcg ctctcccacc ctcaggctcc tcgctggcct ccgcacccgg gcaaaagccg
    12421 ggaggaccgg gacccgcagc gcgacggcct gccgggcccc tgcgcggtgg cacagcctgg
    12481 gcccgctcaa gcggggccgc agggccaagg ggtgcttgcg ccacccacgt cccaggggag
    12541 tccgtggtgg ggctggggcc ggggtcccca ggtcgccggg gcggcgtggg aaccccaagc
    12601 cggggcagct ccacctcccc agcccgcgcc cccggacgcc tccgcctccg cgcggcaggg
    12661 gcagatgcaa ggcatcccgg cgccctccca ggcgctccag gagccggcgc cctggtctgc
    12721 actcccctgc ggcctgctgc tggatgagct cctggcgagc ccggagtttc tgcagcaggc
    12781 gcaacctctc ctagaaacgg aggccccggg ggagctggag gcctcggaag aggccgcctc
    12841 gctggaagca cccctcagcg aggaagaata ccgggctctg ctggaggagc tttaggacgc
    12901 ggggttggga cggggtcggg tggttcgggg cagggccgtg gcctctcttt cgcggggaac
    12961 acctggctgg ctacggaggg gcgtgtctcc gccccgcccc ctccaccggg ctgaccggcc
    13021 tgggattcct gccttctagg tctaggcccg gtgagagact ccacaccgcg gagaactgcc
    13081 attctttcct gggcatcccg gggatcccag agccggccca gcatgctc
//
