LOCUS       sequence-318642-       11368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hyBRET-Epac, an Epac biosensor based on fluorescence resonance 
            energy transfer (FRET) and those based on bioluminescence energy 
            transfer (BRET).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Watabe T, Terai K, Sumiyama K, Matsuda M
  TITLE     Booster, a Red-Shifted Genetically Encoded Forster Resonance Energy 
            Transfer (FRET) Biosensor Compatible with Cyan Fluorescent 
            Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue 
            Light-Responsive Optogenetic Tools.
  JOURNAL   ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941.
  PUBMED    32101394
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Sens. 
            2020 Feb 26. doi: 10.1021/acssensors.9b01941."
COMMENT     Sequence Label: pPBbsr-6080NES(Epac-sensor)
FEATURES             Location/Qualifiers
     source          1..11368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1746..1748,1749..1751,1752..2459)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     CDS             6303..6329
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    6422..6971
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6566..6583)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6793..6812
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(7442..7459)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7448..7672
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7719..7735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7743..7759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7767..7773,7774..7791,7792..7797))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7812..7833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8067..8129
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8170..8190)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8170..8188)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8209..8225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8233..8249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8257..8263,8264..8281,8282..8287))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8302..8323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8440..8457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8611..9199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8691..8710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9370..10161,10162..10230))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9993..10012
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10231..10335)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10361..10816)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10498..10519)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10710..10729
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10943..10965
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10957..10974
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10958..10974
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10984..11003
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10984..11002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   11032..11066
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg ggatccgaat
     1741 tcggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1801 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1861 cctacggcaa gctgaccctg aagcttctat gcaccaccgg caagctgccc gtgccctggc
     1921 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
     1981 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2041 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2101 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2161 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
     2221 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     2281 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2341 accactacct gagctaccag tccgccctgt tcaaagaccc caacgagaag cgcgatcaca
     2401 tggtcctgct ggagttcctg accgccgccg ggatcactga gggcatgaac gagctgtacc
     2461 tcgaccccgt gggaactcat gagatggagg aggagttggc cgaagctgtg gccctgctct
     2521 cccagcgggg gcctgacgcc ctgctcactg tggcacttcg aaagccccca ggtcagcgca
     2581 cggatgaaga gctggacctc atctttgagg agctgctgca catcaaggct gtggcccacc
     2641 tctccaactc ggtgaagcga gaattagcgg ctgttctgct ctttgaacca cacagcaagg
     2701 cagggaccgt gttgttcagc cagggggaca agggcacttc gtggtacatt atctggaagg
     2761 gatctgtcaa cgtggtgacc catggcaagg ggctggtgac caccctgcat gagggagatg
     2821 attttggaca gctggctctg gtgaatgatg caccccgggc agccaccatc atcctgcgag
     2881 aagacaactg tcatttcctg cgtgtggaca agcaggactt caaccgtatc atcaaggatg
     2941 tggaggcaaa gaccatgcgg ctggaagaac atggcaaagt ggtgctggtg ctggagagag
     3001 cctctcaggg cgccggccct tcccgacccc caaccccagg caggaaccgg tatacagtga
     3061 tgtctggcac cccagagaag atcctagagc ttctgttgga ggccatggga ccagattcca
     3121 gtgctcatga cccaacagag acattcctca gcgacttcct cctgacccac agggtcttca
     3181 tgcccagcgc ccaactctgc gctgcccttc tgcaccactt ccatgtggag cctgcgggtg
     3241 gcagcgagca ggagcgcagc acctacgtct gcaacaagag gcagcagatc ttgcggctgg
     3301 tcagccagtg ggtggccctg tatggctcca tgctccacac tgaccctgtg gccaccagct
     3361 tcctccagaa actctcagac ctggtgggca gggacacccg actcagcaac ctgctgaggg
     3421 agcagtggcc agagaggcgg cgatgccaca ggttggagaa tggctgtggg aatgcatctc
     3481 ctcagatgaa ggcccggaac ttgcctgttt ggctccccaa ccaggacgag ccccttcctg
     3541 gcagcagctg tgccatccaa gttggggata aagtccccta tgacatctgc cggccagacc
     3601 actcagtgtt gaccctgcag ctgcctgtga cagcctccgt gagagaggtg atggcagcgt
     3661 tggcccagga ggatggctgg accaaggggc aggtgctggt gaaggtcaat tctgcaggtg
     3721 atgccattgg cctgcagcca gatgcccgtg gtgtggccac atctctgggg ctcaatgagc
     3781 gtctctttgt tgtcaaccca caggaagcgc atgagctgat cccacaccct gaccagctgg
     3841 ggcccactgt gggctctgct gaggggctgg acctggtgag tgccaaggac ctggcaggcc
     3901 agctgacgga ccacgactgg agcctcttca acagtatcca ccaggtggag ctgatccact
     3961 atgtgctggg cccccagcat ctgcgggatg tcaccaccgc caacctggag cgcttcatgc
     4021 gccgcttcaa tgagctgcag tactgggtgg ccaccgagct gtgtctctgc cccgtgcccg
     4081 gcccccgggc ccagctgctc aggaagttca ttaagctggc ggcccacctc aaggagcaga
     4141 agaatctcaa ttccttcttt gccgtcatgt ttggcctcag caactcggcc atcagccgcc
     4201 tagcccacac ctgggagcgg ctgccccaca aagtccggaa gctgtactcc gccctcgaga
     4261 ggctgctgga tccctcatgg aaccaccggg tataccgact ggccctcgcc aagctctccc
     4321 ctcctgtcat ccccttcatg ccccttcttc tcaaagacat gaccttcatt catgagggaa
     4381 accacacact agtggagaat ctcatcaact ttgagaagat gagaatgatg gccagagccg
     4441 cgcggatgct gcaccactgc cgaagccaca accctgtgcc tctctcacca ctcagaagcc
     4501 gagtttccca cctccacgag gacagccagg tggcgaggat ttccacatgc tcggagcagt
     4561 ccctgagcac ccggagtcca gccagcacct gggcttatgt ccagcagctg aaggtcattg
     4621 acaaccagcg ggaactctcc cgcctctccc gagagctgga gccaggcggc cgcatggtga
     4681 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4741 tcaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4801 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4861 ccaccctgtc ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     4921 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4981 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     5041 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     5101 agtacaacta cttcagcggg aacgtctata tcaccgccga caagcagaag aacggcatca
     5161 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     5221 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     5281 gcacccagtc cgccttaagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     5341 agttcttgac cgccgccggg ggtaccaagg tgtacgaccc cgagcagagg aagaggatga
     5401 tcaccggccc ccagtggtgg gccaggtgca agcagatgaa cgtgctggac agcttcatca
     5461 actactacga cagcgagaag cacgccgaga acgccgtgat cttcctgcac ggcaacgcca
     5521 ctagcagcta cctgtggagg cacgtggtgc cccacatcga gcccgtggcc aggtgcatca
     5581 tccccgatct gatcggcatg ggcaagagcg gcaagagcgg caacggcagc tacaggctgc
     5641 tggaccacta caagtacctg accgcctggt tcgagcttct gaacctgccc aagaagatca
     5701 tcttcgtggg ccacgactgg ggcgccgccc tggccttcca ctacgcctac gagcaccagg
     5761 acaggatcaa ggccatcgtg cacatggaga gcgtggtgga cgtgatcgag agctgggacg
     5821 agtggccaga catcgaggag gacatcgccc tgatcaagag cgaggagggc gagaagatgg
     5881 tgctggagaa caacttcttc gtggagaccg tgctgcccag caagatcatg agaaagctgg
     5941 agcccgagga gttcgccgcc tacctggagc ccttcaagga gaagggcgag gtgagaagac
     6001 ccaccctgag ctggcccaga gagatccccc tggtgaaggg cggcaagccc gacgtggtgc
     6061 agatcgtgag aaactacaac gcctacctga gagccagcga cgacctgccc aagctgttca
     6121 tcgagggcga ccccggcttc ttcagcaacg ccatcgtgga gggcgccaag aagttcccca
     6181 acaccgagtt cgtgaaggtg aagggcctgc acttcctcca ggaggacgcc cccgacgaga
     6241 tgggcaagta catcaagagc ttcgtggaga gagtgctgaa gaacgagcag tctagactgc
     6301 agctgcctcc tctggaacgc ctgactctgg attaagtcga cgggccgcgg taacaattgt
     6361 taactaactt aagctagcaa cggtttccct ctagcgggat caattccgcc ccccccccct
     6421 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     6481 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     6541 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     6601 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     6661 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     6721 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     6781 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     6841 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     6901 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     6961 acacgataat accatggtca tgaaaacatt taacatttct caacaagatc tagaattagt
     7021 agaagtagcg acagagaaga ttacaatgct ttatgaggat aataaacatc atgtgggagc
     7081 ggcaattcgt acgaaaacag gagaaatcat ttcggcagta catattgaag cgtatatagg
     7141 acgagtaact gtttgtgcag aagccattgc gattggtagt gcagtttcga atggacaaaa
     7201 ggattttgac acgattgtag ctgttagaca cccttattct gacgaagtag atagaagtat
     7261 tcgagtggta agtccttgtg gtatgtgtag ggagttgatt tcagactatg caccagattg
     7321 ttttgtgtta atagaaatga atggcaagtt agtcaaaact acgattgaag aactcattcc
     7381 actcaaatat acccgaaatt aaaagtcgag atgcatcgat gatctagagc tcgctgatca
     7441 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7501 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7561 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7621 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     7681 gcggaaagaa cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     7741 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7801 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7861 cagtcgggaa acctgtcgtg ccagcggatc ttaaaagttt tgttacttta tagaagaaat
     7921 tttgagtttt tgtttttttt taataaataa ataaacataa ataaattgtt tgttgaattt
     7981 attattagta tgtaagtgta aatataataa aacttaatat ctattcaaat taataaataa
     8041 acctcgatat acagaccgat aaaacacatg cgtcaatttt acgcatgatt atctttaacg
     8101 tacgtcacaa tatgattatc tttctagggt taatctagta tacgcgttta attaatccag
     8161 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     8221 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8281 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8341 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8401 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8461 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8521 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8581 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9121 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9181 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9241 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9301 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9361 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9421 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9481 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9541 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9601 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9661 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9721 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9781 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9841 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9901 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9961 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10021 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10081 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10141 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10201 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10261 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10321 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
    10381 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
    10441 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
    10501 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
    10561 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
    10621 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
    10681 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
    10741 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
    10801 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
    10861 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    10921 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
    10981 gcgtaatacg actcactata gggcgaattg gggcgcgcca ttctagatta accctagaaa
    11041 gatagtctgc gtaaaattga cgcatgcatt cttgaaatat tgctctctct ttctaaatag
    11101 cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc gcttggagct cccgtgaggc
    11161 gtgcttgtca atgcggtaag tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa
    11221 tgacgcatga ttatctttta cgtgactttt aagatttaac tcatacgata attatattgt
    11281 tatttcatgt tctacttacg tgataactta ttatatatat attttcttgt tatagatatc
    11341 aactagaatg ctagcatggg cccatctc
//
