LOCUS       sequence-318662-        9648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating transgenic mice expressing ROCK biosensor based on 
            FRET..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Imanishi A, Ichise H, Fan C, Nakagawa Y, Kuwahara K, Sumiyama K, 
            Matsuda M, Terai K
  TITLE     Visualization of Spatially Controlled Vasospasm by Sympathetic 
            Nerve-Mediated ROCK Activation.
  JOURNAL   Am J Pathol. 2020 Oct 15. pii: S0002-9440(20)30459-4. doi: 
            10.1016/j.ajpath.2020.09.012.
  PUBMED    33069718
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Pathol. 2020 Oct 15. pii: S0002-9440(20)30459-4. doi: 
            10.1016/j.ajpath.2020.09.012."
COMMENT     Sequence Label: pT2ADW-4146NES(ROCK)
FEATURES             Location/Qualifiers
     source          1..9648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1734..1736,1737..1739,1740..2447)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     CDS             2883..3230
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             join(3291..3293,3294..3296,3297..3809)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             4020..4046
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    4070..4658
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4123..4143)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4541..4552)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4723..4857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4747..4766)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4801..4820
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(4979..5141,5142..5153)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5183..5202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5184..5202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5212..5229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5212..5228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5221..5243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5370..5824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5456..5475)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5666..5687
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5850..5954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5955..6023,6024..6815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6173..6192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6986..7574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7475..7494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7728..7745
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7862..7883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7898..7903,7904..7921,7922..7928)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     7960..7976
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7960..7976
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7995..8015
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7997..8015
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(8072..8083,8084..8271)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc ggcatggtga
     1741 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1801 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1861 tgaccctgaa gcttctatgc accaccggca agctgcccgt gccctggccc accctcgtga
     1921 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     1981 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2041 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2101 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2161 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     2221 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     2281 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2341 gctaccagtc cgccctgttc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2401 agttcctgac cgccgccggg atcactgagg gcatgaacga gctgtacctc gagaagtttt
     2461 ctcaagaaca gatcggcgaa aacattgtgt gcagggtcat ttgtaccacg ggtcaaattc
     2521 ccatccgaga tttgtcagct gatatttcac aagtgcttaa ggaaaaacga tccataaaga
     2581 aagtttggac atttggtaga aacccagcct gtgactatca tttaggaaac atttcaagac
     2641 tgtcaaataa gcatttccaa atactactag gagaagacgg taacctttta ttgaatgaca
     2701 tttccactaa tgggacctgg ttaaatgggc aaaaagtcga gaagaacagc aatcagttac
     2761 tgtcccaagg tgatgaaata accgttggtg taggcgtgga atcagatatt ttatctctgg
     2821 tcattttcat aaacgacaaa tttaagcagt gcctggagca gaacaaagtt gatcgcggta
     2881 ccagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg
     2941 gttccggcag tgctggtggt agtgctggtg gtagtaccag tgctggtggt agtgctggtg
     3001 gtagtgctgg tggtagtgct ggtggtagtg ctggtggttc cggcagtgct ggtggtagtg
     3061 ctggtggtag taccagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg
     3121 gtagtgctgg tggttccggc agtgctggtg gtagtgctgg tggtagtacc agtgctggtg
     3181 gtagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggt tccggaaaga
     3241 gaagaaacag aagaaagacc ctggtggacc tgcctctgga tggcggccgc atggtgagca
     3301 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     3361 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     3421 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     3481 ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     3541 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3601 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3661 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3721 acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     3781 ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3841 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacttgagca
     3901 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3961 tcgtgaccgc cgccgggatc actctcggca tggacgagct gggccgctct agactgcagc
     4021 tgcctcctct ggaacgcctg actctggatt aagtcgacgg atcctcgata atcaacctct
     4081 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4141 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4201 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4261 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4321 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4381 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4441 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     4501 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4561 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4621 tcagacgagt cggatctccc tttgggccgc ctccccgcat ctcgacaaag gatccaatgg
     4681 aggccgccac cgcggtggag ctcgaattaa ttcatcgatg atgatccaga catgataaga
     4741 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     4801 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     4861 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     4921 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga tcagatcttt
     4981 cttgctttta cttttacttc cttaatactc aagtacaatt ttaatggagt acttttttac
     5041 ttttactcaa gtaagattct agccagatac ttttactttt aattgagtaa aattttccct
     5101 aagtacttgt actttcactt gagtaaaatt tttgagtact ttttacacct ctgtcaagaa
     5161 ccatatgccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     5221 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5281 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5341 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     5401 gggtgtggtg gttacgcgca gcgtgaccac cacacccgcc gcgccctagc gcccgctcct
     5461 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5521 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5581 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5641 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5701 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5761 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5821 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5881 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5941 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6001 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6061 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6121 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6181 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6241 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6301 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6361 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6421 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6481 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6541 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6601 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6661 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6721 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6781 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6841 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6901 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7621 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7681 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7741 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7801 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7861 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7921 gtatgttgtg tggaattgtg atcggataac aatttcacac aggaaacagc tatgaccatg
     7981 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcggatctg
     8041 ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt aaagtacttg agtaatttta
     8101 cttgattact gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa
     8161 aaatcaatac ttttactttt acttaattac atttttttag aaaaaaaagt actttttact
     8221 ccttacaatt ttatttacag tcaaaaagta cttatttttt ggagatcact tgggccctct
     8281 agatgcatgc cggctggccc aggccatcgg cattccggag cccagggtcc agatttggtt
     8341 tcagaatgag aggtcacgcc agctgaggca gcaccggcgg gaatctcggc cctggcccgg
     8401 gagacgcggc ccgccagaag gccggcgaaa gcggaccgcc gtcaccggat cccagaccgc
     8461 cctgctcctc cgagcctttg agaaggatcg ctttccaggc atcgccgccc gggaggagct
     8521 ggccagagag acgggcctcc cggagtccag gattcagatc tggtttcaga atcgaagggc
     8581 caggcacccg ggacagggtg gcagggcgcc cgcgcaggca ggcggcctgt gcagcgcggc
     8641 ccccggcggg ggtcaccctg ctccctcgtg ggtcgccttc gcccacaccg gcgcgtgggg
     8701 aacggggctt cccgcacccc acgtgccctg cgcgcctggg gctctcccac agggggcttt
     8761 cgtgagccag gcagcgaggg ccgcccccgc gctgcagccc agccaggccg cgccggcaga
     8821 ggggatctcc caacctgccc cggcgcgcgg ggatttcgcc tacgccgccc cggctcctcc
     8881 ggacggggcg ctctcccacc ctcaggctcc tcgctggcct ccgcacccgg gcaaaagccg
     8941 ggaggaccgg gacccgcagc gcgacggcct gccgggcccc tgcgcggtgg cacagcctgg
     9001 gcccgctcaa gcggggccgc agggccaagg ggtgcttgcg ccacccacgt cccaggggag
     9061 tccgtggtgg ggctggggcc ggggtcccca ggtcgccggg gcggcgtggg aaccccaagc
     9121 cggggcagct ccacctcccc agcccgcgcc cccggacgcc tccgcctccg cgcggcaggg
     9181 gcagatgcaa ggcatcccgg cgccctccca ggcgctccag gagccggcgc cctggtctgc
     9241 actcccctgc ggcctgctgc tggatgagct cctggcgagc ccggagtttc tgcagcaggc
     9301 gcaacctctc ctagaaacgg aggccccggg ggagctggag gcctcggaag aggccgcctc
     9361 gctggaagca cccctcagcg aggaagaata ccgggctctg ctggaggagc tttaggacgc
     9421 ggggttggga cggggtcggg tggttcgggg cagggccgtg gcctctcttt cgcggggaac
     9481 acctggctgg ctacggaggg gcgtgtctcc gccccgcccc ctccaccggg ctgaccggcc
     9541 tgggattcct gccttctagg tctaggcccg gtgagagact ccacaccgcg gagaactgcc
     9601 attctttcct gggcatcccg gggatcccag agccggccca gcatgctc
//
