LOCUS       sequence-318664-        9436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high 
            affinity G-protein coupled receptor that mobilizes Ca2+ from 
            intracellular stores upon binding extracellular ATP.48,49 By using 
            the Gibson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Mazzarda F, D'Elia A, Massari R, De Ninno A, Bertani FR, Businaro L,
            Ziraldo G, Zorzi V, Nardin C, Peres C, Chiani F, Tettey-Matey A, 
            Raspa M, Scavizzi F, Soluri A, Salvatore AM, Yang J, Mammano F
  TITLE     Organ-on-chip model shows that ATP release through connexin 
            hemichannels drives spontaneous Ca(2+) signaling in non-sensory 
            cells of the greater epithelial ridge in the developing cochlea.
  JOURNAL   Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc00427h.
  PUBMED    32700707
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Lab Chip. 
            2020 Jul 23. doi: 10.1039/d0lc00427h."
COMMENT     Sequence Label: pHOT-P2Y2R
FEATURES             Location/Qualifiers
     source          1..9436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             88..795
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(233..251)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     501..520
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             805..861
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             883..936
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    2099..2687
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2152..2172)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2570..2581)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2778..3011
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3083..3204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3120..3139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3174..3193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3244..3379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3399..3418)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3400..3418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3428..3445)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3428..3444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3437..3459)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3586..4041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3673..3692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3883..3904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4067..4171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4172..4240,4241..5032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4390..4409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5203..5791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5692..5711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5945..5962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6079..6100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6115..6120,6121..6138,6139..6145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6153..6169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6158..6180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6177..6193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6177..6193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6212..6232
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6214..6232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6260..6486
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6487..6667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6714..6839
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7332..7565
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7750..7794
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8092..8209
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(8274..9436,1..49)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     9322..9341
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc
       61 tgcaggtccg atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
      121 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      181 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      241 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      301 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      361 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      421 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      481 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      541 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
      601 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
      661 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
      721 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
      781 gacgagctgt acaagggatc tggagcaaca aacttctcac tactcaaaca agcaggtgac
      841 gtggaggaga atcccgggcc ttctagagga tccggctccg gcgagggcag gggaagtcta
      901 ctaacatgcg gggacgtgga ggaaaatccc ggcccaggca tggcagcaga cctgggcccc
      961 tggaatgaca ccatcaatgg cacctgggat ggggatgagc tgggctacag gtgccgcttc
     1021 aacgaggact tcaagtacgt gctgctgcct gtgtcctacg gcgtggtgtg cgtgcttggg
     1081 ctgtgtctga acgccgtggc gctctacatc ttcttgtgcc gcctcaagac ctggaatgcg
     1141 tccaccacat atatgttcca cctggctgtg tctgatgcac tgtatgcggc ctccctgccg
     1201 ctgctggtct attactacgc ccgcggcgac cactggccct tcagcacggt gctctgcaag
     1261 ctggtgcgct tcctcttcta caccaacctt tactgcagca tcctcttcct cacctgcatc
     1321 agcgtgcacc ggtgtctggg cgtcttacga cctctgcgct ccctgcgctg gggccgggcc
     1381 cgctacgctc gccgggtggc cggggccgtg tgggtgttgg tgctggcctg ccaggccccc
     1441 gtgctctact ttgtcaccac cagcgcgcgc gggggccgcg taacctgcca cgacacctcg
     1501 gcacccgagc tcttcagccg cttcgtggcc tacagctcag tcatgctggg cctgctcttc
     1561 gcggtgccct ttgccgtcat ccttgtctgt tacgtgctca tggctcggcg actgctaaag
     1621 ccagcctacg ggacctcggg cggcctgcct agggccaagc gcaagtccgt gcgcaccatc
     1681 gccgtggtgc tggctgtctt cgccctctgc ttcctgccat tccacgtcac ccgcaccctc
     1741 tactactcct tccgctcgct ggacctcagc tgccacaccc tcaacgccat caacatggcc
     1801 tacaaggtta cccggccgct ggccagtgct aacagttgcc ttgaccccgt gctctacttc
     1861 ctggctgggc agaggctcgt acgctttgcc cgagatgcca agccacccac tggccccagc
     1921 cctgccaccc cggctcgccg caggctgggc ctgcgcagat ccgacagaac tgacatgcag
     1981 aggataggag atgtgttggg cagcagtgag gactctaggc ggacagagtc cacgccggct
     2041 ggtagcgaga acactaagga cattcggctg tagaacgttc cggagtcgtc gactcgacaa
     2101 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2161 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2221 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     2281 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     2341 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     2401 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2461 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     2521 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     2581 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcatcttcg
     2641 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     2701 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     2761 aaaagaaaag gggggactgg aagggctacg taactcccaa cgaagacaag atctgctttt
     2821 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2881 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     2941 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3001 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     3061 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     3121 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3181 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     3241 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3301 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3361 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     3421 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3481 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3541 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     3601 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3661 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3721 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3781 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3841 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3901 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3961 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4021 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4081 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4141 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4201 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4261 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4321 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4381 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4441 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4501 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4561 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4621 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4681 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4741 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4801 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4861 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4921 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4981 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5041 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5101 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5161 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5221 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5281 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5341 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5401 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5461 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5521 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5581 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5641 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5701 ctggtatctt tatagtcctg tcgggtttcg ccacctctca cttgagcgtc gatttttgtg
     5761 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5821 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5881 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5941 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     6001 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6061 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6121 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6181 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     6241 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     6301 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     6361 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     6421 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     6481 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6541 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6601 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6661 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     6721 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     6781 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     6841 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     6901 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     6961 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     7021 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     7081 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     7141 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     7201 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     7261 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     7321 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     7381 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     7441 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     7501 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     7561 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     7621 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     7681 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     7741 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     7801 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     7861 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     7921 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     7981 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     8041 tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag
     8101 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     8161 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     8221 cagggacagc agagatccag tttggttaat taacccgtgt cggctccaga tctggcctcc
     8281 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
     8341 agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg
     8401 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
     8461 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
     8521 gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata
     8581 taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt
     8641 cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg
     8701 gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc
     8761 tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa
     8821 atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt
     8881 gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat
     8941 gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga
     9001 agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg
     9061 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt
     9121 cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg
     9181 cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg
     9241 cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat
     9301 gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga
     9361 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
     9421 attttcagtg ttagac
//
