LOCUS       sequence-318666-        2712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0541
FEATURES             Location/Qualifiers
     source          1..2712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             80..733
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     691..713
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             740..757
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(825..841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(825..841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(838..860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1013..1601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1093..1112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1734..1828)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1849..2508)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2423..2442
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2509..2611)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     join(2698..2712,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(2712,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa gtgccattcc
       61 gcctgaccta ggtctctcca tgtcaccgat tttaggctac tggaaaatta agggtcttgt
      121 gcaacccaca cgtctgctgc tggagtactt agaggagaag tacgaggagc acctctacga
      181 acgcgacgag ggcgacaagt ggcgtaataa gaaattcgag ttaggcttag aattccctaa
      241 cctgccgtac tacatcgacg gcgacgtcaa gctgacccaa agtatggcga ttatccgcta
      301 cattgccgat aaacataata tgctcggcgg ctgcccgaag gaacgcgctg aaatctccat
      361 gttagagggc gctgtcctgg acatccgtta tggggtgtca cgtatcgcgt actcgaagga
      421 tttcgagact ctgaaggtgg acttcctgtc gaaattaccc gagatgttaa agatgtttga
      481 ggaccgcttg tgccacaaga cttacctgaa cggcgaccac gtgactcacc cggattttat
      541 gctgtacgat gccttggacg tcgtactgta tatggatccg atgtgtttgg acgcctttcc
      601 gaagctggtg tgcttcaaga agcgcatcga ggccattccg cagatcgaca aatatttaaa
      661 gtcgtcaaaa tacattgcgt ggccgcttca gggctggcaa gccacgtttg gtggcgggga
      721 tcacccacct aagagcgacc tggttccgcg tggatcaatg agagacctag gctaggtgga
      781 ggctcagtga tgataagtct gcgatggtgg atgcatgtgt catggtcata gctgtttcct
      841 gtgtgaaatt gttatccgct cagagggcac aatcctattc cgcgctatcc gacaatctcc
      901 aagacattag gtggagttca gttcggcgta tggcatatgt cgctggaaag aacatgtgag
      961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1441 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1501 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1561 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1621 ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag cgtaaatggg
     1681 tagggggctt caaatcgtcc ccccatacga tataagttgt tactagtgct tggattctca
     1741 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1801 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     1861 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     1921 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     1981 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     2041 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     2101 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     2161 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     2221 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     2281 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     2341 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     2401 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     2461 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     2521 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     2581 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tgatacctga aacaaaaccc
     2641 atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca gggttttccc
     2701 agtcacgacg tt
//
