LOCUS       sequence-318667-        2748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0542
FEATURES             Location/Qualifiers
     source          1..2748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             119..769
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKF
                     ELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYG
                     VSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLY
                     MDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     727..749
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             776..793
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(861..877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(861..877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(874..896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1049..1637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1129..1148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1770..1864)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1885..2544)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2459..2478
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2545..2647)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     join(2734..2748,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(2748,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa gtgccattcc
       61 gcctgaccta ggtctcacca tggtaagcgg ctggcgtctg ttcaagaaaa ttagcggatc
      121 accgatttta ggctactgga aaattaaggg tcttgtgcaa cccacacgtc tgctgctgga
      181 gtacttagag gagaagtacg aggagcacct ctacgaacgc gacgagggcg acaagtggcg
      241 taataagaaa ttcgagttag gcttagaatt ccctaacctg ccgtactaca tcgacggcga
      301 cgtcaagctg acccaaagta tggcgattat ccgctacatt gccgataaac ataatatgct
      361 cggcggctgc ccgaaggaac gcgctgaaat ctccatgtta gagggcgctg tcctggacat
      421 ccgttatggg gtgtcacgta tcgcgtactc gaaggatttc gagactctga aggtggactt
      481 cctgtcgaaa ttacccgaga tgttaaagat gtttgaggac cgcttgtgcc acaagactta
      541 cctgaacggc gaccacgtga ctcacccgga ttttatgctg tacgatgcct tggacgtcgt
      601 actgtatatg gatccgatgt gtttggacgc ctttccgaag ctggtgtgct tcaagaagcg
      661 catcgaggcc attccgcaga tcgacaaata tttaaagtcg tcaaaataca ttgcgtggcc
      721 gcttcagggc tggcaagcca cgtttggtgg cggggatcac ccacctaaga gcgacctggt
      781 tccgcgtgga tcaatgagag acctaggcta ggtggaggct cagtgatgat aagtctgcga
      841 tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaga
      901 gggcacaatc ctattccgcg ctatccgaca atctccaaga cattaggtgg agttcagttc
      961 ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1021 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1081 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1261 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1381 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1441 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1501 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1561 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1621 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctctattca
     1681 acaaagccgc cgtcccgtca agtcagcgta aatgggtagg gggcttcaaa tcgtcccccc
     1741 atacgatata agttgttact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     1801 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     1861 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
     1921 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     1981 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     2041 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     2101 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     2161 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     2221 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     2281 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     2341 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     2401 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     2461 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     2521 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     2581 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     2641 ccgatcaacg tctcattgat acctgaaaca aaacccatcg tacggccaag gaagtctcca
     2701 ataactgtga tccaccacaa gcgccagggt tttcccagtc acgacgtt
//
