LOCUS       sequence-318688-        3198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0544
FEATURES             Location/Qualifiers
     source          1..3198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1229..1240
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1311..1327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1311..1327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1324..1346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1499..2087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1579..1598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2220..2314)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2335..2994)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2909..2928
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2995..3097)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     join(3184..3198,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3198,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa gtgccattcc
       61 gcctgaccta ggtctctcca tgaaaatcga agaaggcaaa ttagtgatat ggatcaatgg
      121 agataaggga tacaatggct tagcggaagt gggaaagaag tttgaaaagg acactgggat
      181 caaggttacg gtcgaacacc cagacaagtt agaggaaaag tttccgcaag tagcagccac
      241 aggggacggt ccggatatca ttttctgggc ccatgatcgt ttcggcggat atgcccagag
      301 tggtctttta gcggagatta ctcctgataa ggcatttcaa gataaattgt accctttcac
      361 gtgggacgcg gttcgctata atggaaaact tatcgcatat cctattgcgg tagaggcact
      421 gagtctcatc tacaataagg acttgctccc taatccgcct aagacatggg aagaaattcc
      481 cgccctcgac aaggagctca aggcaaaggg caaatccgcc ttgatgttta atctccagga
      541 gccatatttt acttggccgt tgatcgcggc cgatggcggc tacgccttta aatacgagaa
      601 tggtaaatat gatatcaagg atgtcggagt agacaatgcg ggtgcaaagg ctggacttac
      661 tttcttagtg gatttgatca agaataagca tatgaacgct gatacggact atagtattgc
      721 cgaggcggcg ttcaacaagg gtgagactgc catgactatt aatggacctt gggcttggtc
      781 gaatattgat acgagtaagg tcaactacgg cgtgactgtg ttacctacat ttaaaggcca
      841 gcctagcaag ccgtttgtgg gtgttttgtc ggcgggcatc aatgcggcat caccgaataa
      901 ggaattagcg aaggaattct tggagaatta ccttctcacg gacgagggcc ttgaggccgt
      961 aaacaaggat aagccattag gcgcggtggc cttaaagagc tatgaagagg aactggtcaa
     1021 ggacccgcgc atcgcagcta ccatggagaa tgctcaaaag ggcgagatta tgcccaatat
     1081 tcctcaaatg tcggcctttt ggtacgcggt ccgcaccgca gtaattaatg cagcgtccgg
     1141 acgccaaacc gtggacgagg cgcttaagga tgctcaaact aattcgagct cgaacaacaa
     1201 caacaataac aataacaaca acctcgggat cgagggaagg ggaatgagag acctaggcta
     1261 ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg
     1321 tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca
     1381 atctccaaga cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca
     1441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1681 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1921 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2101 tcttttctac ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta
     2161 aatgggtagg gggcttcaaa tcgtcccccc atacgatata agttgttact agtgcttgga
     2221 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     2281 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     2341 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     2401 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     2461 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     2521 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     2581 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     2641 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     2701 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     2761 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     2821 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     2881 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     2941 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     3001 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     3061 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattgat acctgaaaca
     3121 aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt
     3181 tttcccagtc acgacgtt
//
