LOCUS       sequence-318690-        9826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag ITK
FEATURES             Location/Qualifiers
     source          1..9826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    805..1004
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1205..1621
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1551..1573
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1589..1605
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1645..1686
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1711..1734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1741..1765
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3626..3650)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3709..4170
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3765..3782)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3992..4011
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4175..4969
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4229..4248)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4839..4858
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5156..5625
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5661..5765
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5766..5834,5835..6626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5984..6003)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8392..8980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8881..8900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9134..9151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9255..9682)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9365..9386)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9577..9596
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgcg
      601 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
      661 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
      721 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
      781 accaccgacc caccaccggg aggcaagctg gccagcaact tatctgtgtc tgtccgattg
      841 tctagtgtct atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg
      901 tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag
      961 acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg
     1021 gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc
     1081 gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca
     1141 gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     1201 ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1261 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1321 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1381 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1441 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1501 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1561 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1621 cggccggatc cagaattcgc caccatgggg tcttcaaaat ctaaaccaaa ggaccccagc
     1681 cagcgccggc gcaggatccg aggttacctt gactacaaag acgatgacga caagcaattg
     1741 acaagtttgt acaaaaaagt tggcatgaac aactttatcc tcctggaaga acagctcatc
     1801 aagaaatccc aacaaaagag aagaacttct ccctcgaact ttaaagtccg cttctttgtg
     1861 ttaaccaaag ccagcctggc atactttgaa gatcgtcatg ggaagaagcg cacgctgaag
     1921 gggtccattg agctctcccg aatcaaatgt gttgagattg tgaaaagtga catcagcatc
     1981 ccatgccact ataaataccc gtttcaggtg gtgcatgaca actacctcct atatgtgttt
     2041 gctccagatc gtgagagccg gcagcgctgg gtgctggccc ttaaagaaga aacgaggaat
     2101 aataacagtt tggtgcctaa atatcatcct aatttctgga tggatgggaa gtggaggtgc
     2161 tgttctcagc tggagaagct tgcaacaggc tgtgcccaat atgatccaac caagaatgct
     2221 tcaaagaagc ctcttcctcc tactcctgaa gacaacaggc gaccactttg ggaacctgaa
     2281 gaaactgtgg tcattgcctt atatgactac caaaccaatg atcctcagga actcgcactg
     2341 cggcgcaacg aagagtactg cctgctggac agttctgaga ttcactggtg gagagtccag
     2401 gacaggaatg ggcatgaagg atatgtacca agcagttatc tggtggaaaa atctccaaat
     2461 aatctggaaa cctatgagtg gtacaataag agtatcagcc gagacaaagc tgaaaaactt
     2521 cttttggaca caggcaaaga aggagccttc atggtaaggg attccaggac tgcaggaaca
     2581 tacaccgtgt ctgttttcac caaggctgtt gtaagtgaga acaatccctg tataaagcat
     2641 tatcacatca aggaaacaaa tgacaatcct aagcgatact atgtggctga aaagtatgtg
     2701 ttcgattcca tccctcttct catcaactat caccaacata atggaggagg cctggtgact
     2761 cgactccggt atccagtttg ttttgggagg cagaaagccc cagttacagc agggctgaga
     2821 tacgggaaat gggtgatcga cccctcagag ctcacttttg tccaagagat tggcagtggg
     2881 caatttgggt tggtgcatct gggctactgg ctcaacaagg acaaggtggc tatcaaaacc
     2941 attcgggaag gggctatgtc agaagaggac ttcatagagg aggctgaagt aatgatgaaa
     3001 ctctctcatc ccaaactggt gcagctgtat ggggtgtgcc tggagcaggc ccccatctgc
     3061 ctggtgtttg agttcatgga gcacggctgc ctgtcagatt atctacgcac ccagcgggga
     3121 ctttttgctg cagagaccct gctgggcatg tgtctggatg tgtgtgaggg catggcctac
     3181 ctggaagagg catgtgtcat ccacagagac ttggctgcca gaaattgttt ggtgggagaa
     3241 aaccaagtca tcaaggtgtc tgactttggg atgacaaggt tcgttctgga tgatcagtac
     3301 accagttcca caggcaccaa attcccggtg aagtgggcat ccccagaggt tttctctttc
     3361 agtcgctata gcagcaagtc cgatgtgtgg tcatttggtg tgctgatgtg ggaagttttc
     3421 agtgaaggca aaatcccgta tgaaaaccga agcaactcag aggtggtgga agacatcagt
     3481 accggatttc ggttgtacaa gccccggctg gcctccacac acgtctacca gattatgaat
     3541 cactgctgga aagagagacc agaagatcgg ccagccttct ccagactgct gcgtcaactg
     3601 gctgaaattg cagaatcagg acttttgcca actttcttgt acaaagtggt ttgatctagt
     3661 cgaccaattc cggttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     3721 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3781 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3841 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3901 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3961 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     4021 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     4081 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
     4141 gtggttttcc tttgaaaaac acgatgataa taccatgatt gaacaagatg gattgcacgc
     4201 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4261 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4321 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     4381 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4441 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4501 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4561 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4621 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4681 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     4741 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4801 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4861 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4921 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     4981 gggttcgcat cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     5041 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
     5101 aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa
     5161 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     5221 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     5281 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
     5341 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     5401 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     5461 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     5521 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     5581 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacaggc cagtggggcc
     5641 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5701 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5761 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5821 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5881 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5941 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6001 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6061 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6121 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6181 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6241 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6301 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6361 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6421 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6481 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6541 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6601 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6661 ttgatttgcg gccgcgaaaa ggacaagcag cgaaaattca cgcccccttg ggaggtggcg
     6721 gcatatgcaa aggatagcac tcccactcta ctactgggta tcatatgctg actgtatatg
     6781 catgaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
     6841 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     6901 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     6961 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta tccagatatt
     7021 tgggtagtat atgctaccca tggcaacatt agcccaccgt gctctcagcg acctcgtgaa
     7081 tatgaggacc aacaaccctg tgcttggcgc tcaggcgcaa gtgtgtgtaa tttgtcctcc
     7141 agatcgcagc aatcgcgccc ctatcttggc ccgcccacct acttatgcag gtattccccg
     7201 gggtgccatt agtggttttg tgggcaagtg gtttgaccgc agtggttagc ggggttacaa
     7261 tcagccaagt tattacaccc ttattttaca gtccaaaacc gcagggcggc gtgtgggggc
     7321 tgacgcgtgc ccccactcca caatttcaaa aaaaagagtg gccacttgtc tttgtttatg
     7381 ggccccattg gcgtggagcc ccgtttaatt ttcgggggtg ttagagacaa ccagtggagt
     7441 ccgctgctgt cggcgtccac tctctttccc cttgttacaa atagagtgta acaacatggt
     7501 tcacctgtct tggtccctgc ctgggacaca tcttaataac cccagtatca tattgcacta
     7561 ggattatgtg ttgcccatag ccataaattc gtgtgagatg gacatccagt ctttacggct
     7621 tgtccccacc ccatggattt ctattgttaa agatattcag aatgtttcat tcctacacta
     7681 gtatttattg cccaaggggt ttgtgagggt tatattggtg tcatagcaca atgccaccac
     7741 tgaacccccc gtccaaattt tattctgggg gcgtcacctg aaaccttgtt ttcgagcacc
     7801 tcacatacac cttactgttc acaactcagc agttattcta ttagctaaac gaaggagaat
     7861 gaagaagcag gcgaagattc aggagagttc actgcccgct ccttgatctt cagccactgc
     7921 ccttgtgact aaaatggttc actaccctcg tggaatcctg accccatgta aataaaaccg
     7981 tgacagctca tggggtggga gatatcgctg ttccttagga cccttttact aaccctaatt
     8041 cgatagcata tgcttcccgt tgggtaacat atgctattga attagggtta gtctggatag
     8101 tatatactac tacccgggaa gcatatgcta cccgtttagg gttaacaagg gggccttata
     8161 aacactattg ctaatgccct cttgagggtc cgcttatcgg tagctacaca ggcccctctg
     8221 attgacgttg gtgtagcctc ccgtagtctt cctgggcccc tgggaggtac atgtcggccg
     8281 caaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8341 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8401 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8461 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8521 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8581 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8641 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8701 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8761 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8821 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8881 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8941 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9001 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9061 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9121 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9181 gcctctcccc gcgcgttggc cgattcatta atgcaactat ggttggcgcg ttgtaaacgt
     9241 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     9301 gaccgaaatc ggcaaaatcc cttataaatc aaaagaatag cccgagatag agttgagtgt
     9361 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     9421 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt
     9481 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     9541 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     9601 cgctaaggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     9661 taatgcgccg ctacagggcg cgccgatctt caatattggc cattagccat attattcatt
     9721 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat
     9781 aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgtt
//
