LOCUS       sequence-318721-        8649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses FAM26E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8649)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_FAM26E_OE_1
FEATURES             Location/Qualifiers
     source          1..8649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    588..612
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1540..1564)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1566..1607
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1649..2237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1702..1722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2120..2131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2309..2542
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2620..2741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2657..2676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2711..2730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2781..2916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2936..2955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2937..2955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2965..2982)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2965..2981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2974..2996)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3123..3578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3210..3229)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3420..3441
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3604..3708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3709..3777,3778..4569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3927..3946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4740..5328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5229..5248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5482..5499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5616..5637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5652..5657,5658..5675,5676..5682)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5690..5706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5695..5717
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5714..5730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5714..5730
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5749..5769
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5751..5769
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5797..6023
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6024..6204
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6251..6376
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6869..7102
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7287..7331
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7506..8006
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7925..7943
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8018..8413
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8473..8590
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctctctg gctaactgtc gggatcaaca agtttgtaca
      601 aaaaagttgg catggatgct tttcagggca ttttaaaatt cttccttaat cagaaaactg
      661 ttattggcta cagcttcatg gctctgctga ccgtgggaag tgagcgtctc ttttctgttg
      721 tggcttttaa gtgcccctgc agcactgaga atatgaccta tgggctggtt ttcctctttg
      781 ctcctgcctg ggtgttactg atcctgggat tctttctgaa caataggtcg tggagactct
      841 tcacaggctg ctgtgtgaat cccaggaaaa tctttcccag aggccacagc tgccgtttct
      901 tctacgtcct cggccagatc actctgagct cattggtggc tccagtgatg tggctttctg
      961 tggctctgct caatggaact ttctatgaat gtgccatgag cgggacgaga agttcaggac
     1021 tcctggaact gatttgcaag ggtaagccca aagagtgctg ggaagaactt cacaaagtat
     1081 cttgtggcaa aactagcatg ctacctaccg tcaatgaaga actgaaactc tcccttcagg
     1141 cccagtctca gattctagga tggtgcctga tttgttcagc gtctttcttc tctctgctca
     1201 ccacatgtta tgctcgctgc cgatctaaag ttagctacct tcagctgagt ttttggaaga
     1261 catatgcaca aaaggagaag gagcagttgg aaaatacatt tctggactat gccaacaagc
     1321 tgagcgagag gaacctgaaa tgtttttttg aaaacaagag gccagatcct tttcccatgc
     1381 ctacgtttgc tgcctgggag gctgcttcag agctgcattc tttccaccaa agccagcaac
     1441 actatagcac cctccacaga gtggtggaca atggtctgca acttagccct gaggatgatg
     1501 agacgacaat ggtccttgtg ggtactgccc acaatatgta cccaactttc ttgtacaaag
     1561 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
     1621 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     1681 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     1741 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     1801 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     1861 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     1921 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     1981 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2041 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     2101 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2161 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2221 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     2281 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     2341 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     2401 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     2461 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     2521 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     2581 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     2641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2701 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     2761 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2821 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2881 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     2941 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     3001 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3061 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3121 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3181 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3241 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3301 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3361 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3421 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3481 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3541 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     3601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3721 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3781 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3841 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3901 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3961 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4021 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4081 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4141 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4201 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4261 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4321 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4381 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4441 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4501 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4561 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4621 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4681 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4861 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5101 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5341 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5401 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5461 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5521 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5581 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     5641 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     5701 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     5761 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     5821 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     5881 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     5941 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     6001 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     6061 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6121 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6181 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     6241 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     6301 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     6361 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     6421 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     6481 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     6541 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     6601 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     6661 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     6721 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     6781 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     6841 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     6901 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     6961 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7021 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7081 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7141 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7201 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7261 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7321 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7381 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     7441 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     7501 gacccttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     7561 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     7621 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     7681 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     7741 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     7801 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     7861 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     7921 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     7981 accgaatcac cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga
     8041 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     8101 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     8161 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     8221 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     8281 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     8341 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     8401 tgtgtgggag ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt
     8461 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     8521 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     8581 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     8641 ggcccgggg
//
