LOCUS       sequence-318723-        9195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9195)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNS3_OE_1
FEATURES             Location/Qualifiers
     source          1..9195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        35..338
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        339..542
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     493..513
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    565..589
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2063..2087)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2089..2130
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2172..2760
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2225..2245)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2643..2654)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2832..3065
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3143..3264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3180..3199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3234..3253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3304..3439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3459..3478)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3460..3478)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3488..3505)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3488..3504)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3497..3519)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3646..4101
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3733..3752)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3943..3964
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4127..4231
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4232..4300,4301..5092)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4450..4469)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5263..5851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5752..5771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6005..6022
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6139..6160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6175..6180,6181..6198,6199..6205)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6213..6229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6218..6240
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6237..6253
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6237..6253
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6272..6292
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6274..6292
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6320..6546
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6547..6727
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6774..6899
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7392..7625
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7810..7854
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8029..8529
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8448..8466
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8541..8936
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8996..9113
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
       61 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      121 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      181 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      241 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      301 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      361 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      421 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      481 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      541 ctctctggct aactgtcggg atcaacaagt ttgtacaaaa aagttggcat ggtgtttggt
      601 gagtttttcc atcgccctgg acaagacgag gaacttgtca acctgaatgt ggggggcttt
      661 aagcagtctg ttgaccaaag caccctcctg cggtttcctc acaccagact ggggaagctg
      721 cttacttgcc attctgaaga ggccattctg gagctgtgtg atgattacag tgtggccgat
      781 aaggaatact actttgatcg gaatccctcc ttgttcagat atgttttgaa tttttattac
      841 acggggaagc tgcatgtcat ggaggagctg tgcgtattct cattctgcca ggagatcgag
      901 tactggggca tcaacgagct cttcattgat tcttgctgca gcaatcgcta ccaggaacgc
      961 aaggaggaaa accacgagaa ggactgggac cagaaaagcc atgatgtgag taccgactcc
     1021 tcgtttgaag agtcgtctct gtttgagaaa gagctggaga agtttgacac actgcgattt
     1081 ggtcagctcc ggaagaaaat ctggattaga atggagaatc cagcgtactg cctgtccgct
     1141 aagcttatcg ctatctcctc cttgagcgtg gtgctggcct ccatcgtggc catgtgcgtt
     1201 cacagcatgt cggagttcca gaatgaggat ggagaagtgg atgatccggt gctggaagga
     1261 ttggagatcg cgtgcattgc ctggttcacc ggggagcttg ccgtccggct ggctgccgct
     1321 ccttgtcaaa agaaattctg gaaaaaccct ctgaacatca ttgactttgt ctctattatt
     1381 cccttctatg ccacgttggc tgtagacacc aaggaggaag agagtgagga tattgagaac
     1441 atgggcaagg tggtccagat cctacggctt atgaggattt tccgaattct aaagcttgcc
     1501 cggcactcgg taggacttcg gtctctaggt gccacactga gacacagcta ccatgaagtt
     1561 gggcttctgc ttctcttcct ctctgtgggc atttccattt tctctgtgct tatctactcc
     1621 gtggagaaag atgaccacac atccagcctc accagcatcc ccatctgctg gtggtgggcc
     1681 accatcagca tgacaactgt gggctatgga gacacccacc cggtcacctt ggcgggaaag
     1741 ctcatcgcca gcacatgcat catctgtggc atcttggtgg tggcccttcc catcaccatc
     1801 atcttcaaca agttttccaa gtactaccag aagcaaaagg acattgatgt ggaccagtgc
     1861 agtgaggatg caccagagaa gtgtcatgag ctaccttact ttaacattag ggatatatat
     1921 gcacagcgga tgcacgcctt cattaccagt ctctcttctg taggcattgt ggtgagcgat
     1981 cctgactcca cagatgcttc aagcattgaa gacaatgagg acatttgtaa caccacctcc
     2041 ttggagaatt gcacagcaaa atgcccaact ttcttgtaca aagtggttgg taagcctatc
     2101 cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc
     2161 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2221 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2281 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2341 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2401 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2461 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2521 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2581 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2641 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2701 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2761 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     2821 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     2881 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2941 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     3001 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     3061 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     3121 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     3181 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3241 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     3301 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3361 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3421 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     3481 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3541 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3601 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     3661 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3721 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3781 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3841 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3901 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3961 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4021 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4081 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4141 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4201 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4261 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4321 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4381 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4441 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4501 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4561 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4621 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4681 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4741 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4801 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4861 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4921 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4981 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5041 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5101 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5161 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5221 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5281 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5341 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5401 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5461 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5521 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5581 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5641 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5701 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5761 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5821 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5881 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5941 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6001 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     6061 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6121 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6181 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6241 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     6301 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     6361 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     6421 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     6481 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     6541 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6601 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6661 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6721 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     6781 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     6841 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     6901 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     6961 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     7021 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     7081 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     7141 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     7201 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     7261 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     7321 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     7381 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     7441 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     7501 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     7561 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     7621 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     7681 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     7741 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     7801 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     7861 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     7921 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     7981 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc
     8041 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     8101 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     8161 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     8221 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     8281 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     8341 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     8401 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     8461 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     8521 tctccccagg gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg
     8581 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     8641 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     8701 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     8761 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     8821 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     8881 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc
     8941 agctgcagta gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     9001 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9061 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9121 attacaggga cagcagagat ccactttggc gccggctcga gggggcccgg ggttattaat
     9181 agtaatcaat tacgg
//
