LOCUS       sequence-318730-        3234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0545
FEATURES             Location/Qualifiers
     source          1..3234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1265..1276
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1347..1363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1347..1363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1360..1382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1535..2123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1615..1634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2256..2350)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2371..3030)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2945..2964
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3031..3133)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     join(3220..3234,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3234,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa gtgccattcc
       61 gcctgaccta ggtctcacca tggtaagcgg ctggcgtctg ttcaagaaaa ttagcggaaa
      121 aatcgaagaa ggcaaattag tgatatggat caatggagat aagggataca atggcttagc
      181 ggaagtggga aagaagtttg aaaaggacac tgggatcaag gttacggtcg aacacccaga
      241 caagttagag gaaaagtttc cgcaagtagc agccacaggg gacggtccgg atatcatttt
      301 ctgggcccat gatcgtttcg gcggatatgc ccagagtggt cttttagcgg agattactcc
      361 tgataaggca tttcaagata aattgtaccc tttcacgtgg gacgcggttc gctataatgg
      421 aaaacttatc gcatatccta ttgcggtaga ggcactgagt ctcatctaca ataaggactt
      481 gctccctaat ccgcctaaga catgggaaga aattcccgcc ctcgacaagg agctcaaggc
      541 aaagggcaaa tccgccttga tgtttaatct ccaggagcca tattttactt ggccgttgat
      601 cgcggccgat ggcggctacg cctttaaata cgagaatggt aaatatgata tcaaggatgt
      661 cggagtagac aatgcgggtg caaaggctgg acttactttc ttagtggatt tgatcaagaa
      721 taagcatatg aacgctgata cggactatag tattgccgag gcggcgttca acaagggtga
      781 gactgccatg actattaatg gaccttgggc ttggtcgaat attgatacga gtaaggtcaa
      841 ctacggcgtg actgtgttac ctacatttaa aggccagcct agcaagccgt ttgtgggtgt
      901 tttgtcggcg ggcatcaatg cggcatcacc gaataaggaa ttagcgaagg aattcttgga
      961 gaattacctt ctcacggacg agggccttga ggccgtaaac aaggataagc cattaggcgc
     1021 ggtggcctta aagagctatg aagaggaact ggtcaaggac ccgcgcatcg cagctaccat
     1081 ggagaatgct caaaagggcg agattatgcc caatattcct caaatgtcgg ccttttggta
     1141 cgcggtccgc accgcagtaa ttaatgcagc gtccggacgc caaaccgtgg acgaggcgct
     1201 taaggatgct caaactaatt cgagctcgaa caacaacaac aataacaata acaacaacct
     1261 cgggatcgag ggaaggggaa tgagagacct aggctaggtg gaggctcagt gatgataagt
     1321 ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     1381 ctcagagggc acaatcctat tccgcgctat ccgacaatct ccaagacatt aggtggagtt
     1441 cagttcggcg tatggcatat gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg
     1501 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1561 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1621 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1681 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1741 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1801 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1861 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1921 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1981 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2041 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2101 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2161 tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt
     2221 ccccccatac gatataagtt gttactagtg cttggattct caccaataaa aaacgcccgg
     2281 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2341 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
     2401 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     2461 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     2521 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     2581 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     2641 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     2701 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     2761 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     2821 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     2881 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     2941 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     3001 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     3061 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     3121 tacgtgccga tcaacgtctc attgatacct gaaacaaaac ccatcgtacg gccaaggaag
     3181 tctccaataa ctgtgatcca ccacaagcgc cagggttttc ccagtcacga cgtt
//
