LOCUS       sequence-318732-        3168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0546
FEATURES             Location/Qualifiers
     source          1..3168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1193..1213
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(1281..1297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1281..1297)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1294..1316)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1469..2057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1549..1568)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2190..2284)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2305..2964)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2879..2898
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2965..3067)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     join(3154..3168,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3168,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa gtgccattcc
       61 gcctgaccta ggtctctcca tgaaaatcga agaaggcaaa ttagtgatat ggatcaatgg
      121 agataaggga tacaatggct tagcggaagt gggaaagaag tttgaaaagg acactgggat
      181 caaggttacg gtcgaacacc cagacaagtt agaggaaaag tttccgcaag tagcagccac
      241 aggggacggt ccggatatca ttttctgggc ccatgatcgt ttcggcggat atgcccagag
      301 tggtctttta gcggagatta ctcctgataa ggcatttcaa gataaattgt accctttcac
      361 gtgggacgcg gttcgctata atggaaaact tatcgcatat cctattgcgg tagaggcact
      421 gagtctcatc tacaataagg acttgctccc taatccgcct aagacatggg aagaaattcc
      481 cgccctcgac aaggagctca aggcaaaggg caaatccgcc ttgatgttta atctccagga
      541 gccatatttt acttggccgt tgatcgcggc cgatggcggc tacgccttta aatacgagaa
      601 tggtaaatat gatatcaagg atgtcggagt agacaatgcg ggtgcaaagg ctggacttac
      661 tttcttagtg gatttgatca agaataagca tatgaacgct gatacggact atagtattgc
      721 cgaggcggcg ttcaacaagg gtgagactgc catgactatt aatggacctt gggcttggtc
      781 gaatattgat acgagtaagg tcaactacgg cgtgactgtg ttacctacat ttaaaggcca
      841 gcctagcaag ccgtttgtgg gtgttttgtc ggcgggcatc aatgcggcat caccgaataa
      901 ggaattagcg aaggaattct tggagaatta ccttctcacg gacgagggcc ttgaggccgt
      961 aaacaaggat aagccattag gcgcggtggc cttaaagagc tatgaagagg aactggccaa
     1021 ggacccgcgc atcgcagcta ccatggagaa tgctcaaaag ggcgagatta tgcccaatat
     1081 tcctcaaatg tcggcctttt ggtacgcggt ccgcaccgca gtaattaatg cagcgtccgg
     1141 acgccaaacc gtggacgagg cgcttaagga tgctcaaacc aactcttcaa gcgagaacct
     1201 gtattttcag tcaatgagag acctaggcta ggtggaggct cagtgatgat aagtctgcga
     1261 tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaga
     1321 gggcacaatc ctattccgcg ctatccgaca atctccaaga cattaggtgg agttcagttc
     1381 ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1441 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1501 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1561 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1621 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1681 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1741 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1801 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1861 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1921 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1981 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2041 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctctattca
     2101 acaaagccgc cgtcccgtca agtcagcgta aatgggtagg gggcttcaaa tcgtcccccc
     2161 atacgatata agttgttact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     2221 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     2281 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
     2341 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     2401 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     2461 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     2521 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     2581 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     2641 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     2701 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     2761 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     2821 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     2881 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     2941 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     3001 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     3061 ccgatcaacg tctcattgat acctgaaaca aaacccatcg tacggccaag gaagtctcca
     3121 ataactgtga tccaccacaa gcgccagggt tttcccagtc acgacgtt
//
