LOCUS       sequence-318738-        2304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPMY0SP0002
FEATURES             Location/Qualifiers
     source          1..2304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             327..1118
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1289..1877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1778..1797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2031..2048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(2127..2150)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(2160..2177)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2219..2237)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac
      121 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
      181 tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
      241 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
      301 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
      361 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
      421 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg
      481 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
      541 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
      601 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
      661 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
      721 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
      781 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
      841 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
      901 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
      961 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     1021 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt
     1081 acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa gtttactcat
     1141 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1201 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1261 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1321 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1381 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1441 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1501 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1561 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1621 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1681 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1741 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1801 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1861 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1921 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1981 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2041 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2101 ttcattaatc actcgtggtc tcacattggg ccctgaaaca gaacttccag accgctgctg
     2161 tgatggtggt gatggtgtgc catggtgaga ccacagtggc tcttcagtgg acgaaagggc
     2221 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2281 ggtggcactt ttcggggaaa tgtg
//
