LOCUS       sequence-318739-        8449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMVp-EGFP-[barcode cloning site]-PGKp-Puro-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Nakayama Y, Fujiu K, Oshima T, Matsuda J, Sugita J, Matsubara TJ, 
            Liu Y, Goto K, Kani K, Uchida R, Takeda N, Morita H, Xiao Y, Hayashi
            M, Maru Y, Hasumi E, Kojima T, Ishiguro S, Kijima Y, Yachie N, 
            Yamazaki S, Yamamoto R, Kudo F, Nakanishi M, Iwama A, Fujiki R, 
            Kaneda A, Ohara O, Nagai R, Manabe I, Komuro I
  TITLE     Heart failure promotes multimorbidity through innate immune memory.
  JOURNAL   Sci Immunol. 2024 May 24;9(95):eade3814. doi: 
            10.1126/sciimmunol.ade3814. Epub 2024 May 24.
  PUBMED    38787963
REFERENCE   2  (bases 1 to 8449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2024-05-24"; volume: "9(95):eade3814. doi"; pages:
            " 10.1126/sciimmunol.ade3814. Epub 2024 May 24"
COMMENT     Sequence Label: pBC001 v3
FEATURES             Location/Qualifiers
     source          1..8449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(167..271)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    319..453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(356..375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     410..429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             621..1254
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1301..1426
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1923..2156
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2341..2385
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2648..2763
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2821..3124
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3125..3328
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3279..3299
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3325..3349
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3429..3431,3432..3434,3435..4148)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3429..3431,3432..3434,3435..4145)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3474..3495)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3735..3754)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4082..4103
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4305..4804
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4340..4363)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4737..4756
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4824..5423
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4824..4843)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5320..5340
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5437..6025
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5490..5510)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5908..5919)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6232..6465
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6594..6610)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6594..6610)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6607..6629)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6618..6634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6642..6648,6649..6666,6667..6672))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6687..6708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6825..6842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6996..7584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7076..7095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7755..8449,1..97,98..166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8378..8397
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
       61 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
      121 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
      181 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
      241 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      301 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      361 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      421 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
      481 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
      541 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
      601 tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct
      661 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
      721 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
      781 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
      841 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
      901 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
      961 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     1021 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     1081 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     1141 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     1201 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1261 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1321 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1381 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1441 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1501 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1561 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1621 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1681 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1741 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1801 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1861 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1921 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1981 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2041 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2101 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2161 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2221 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2281 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2341 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2401 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2461 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2521 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2581 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     2641 tatcgccttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2701 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2761 ttttcgggtt tattacaggg acagcagaga tccagtttat cgataagctt gggagttccg
     2821 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2881 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2941 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3001 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3061 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3121 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3181 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3241 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3301 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     3361 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tctatttccg
     3421 gtgaattcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3481 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3541 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     3601 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     3661 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3721 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     3781 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     3841 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     3901 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     3961 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     4021 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     4081 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     4141 acaagtaata actataacgg tcctaaggta gcgaatctag atgggatcca ttaccctgtt
     4201 atccctactc gaactcgcat acctctgata acaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4261 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gatccaattc taccgggtag gggaggcgct
     4321 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     4381 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     4441 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     4501 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     4561 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     4621 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     4681 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     4741 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     4801 ttcgacctgc agttaacgcc gccatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     4861 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     4921 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     4981 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     5041 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     5101 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     5161 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     5221 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     5281 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     5341 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     5401 tgacccgcaa gcccggtgcc tagacgcgtc tggaacaatc aacctctgga ttacaaaatt
     5461 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5521 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5581 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5641 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5701 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5761 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5821 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     5881 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5941 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6001 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     6061 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     6121 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     6181 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     6241 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6301 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6361 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6421 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6481 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     6541 gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6601 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6661 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6721 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6781 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7621 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7681 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7741 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7801 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7861 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7921 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7981 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8041 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8101 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8161 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8221 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8281 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8341 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8401 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactct
//
