LOCUS       sequence-318740-        2747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS part for MoClo assembly, AATG-TTCG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0539
FEATURES             Location/Qualifiers
     source          1..2747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             82..789
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     primer_bind     complement(860..876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(860..876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(873..895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1048..1636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1128..1147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1769..1863)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1884..2543)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2458..2477
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2544..2646)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     join(2733..2747,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(2747,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtcat gcataatctg cacgcatctg gaataaggaa gtgccattcc
       61 gcctgaccta ggtctctaat gggtgagtct ctgttcaaag gcccacgcga ctataatccc
      121 atctcctcga cgatctgcca cttaaccaac gagtcggacg gacatacgac cagtctgtac
      181 ggcatcgggt tcggcccgtt tattatcact aataaacatc tgttccgtcg caacaacggt
      241 accttacttg tacagtctct tcacggcgtg tttaaagtga agaatactac tacccttcag
      301 cagcatctga tcgacggtcg cgatatgatt atcatccgta tgccgaaaga ctttccgccc
      361 ttcccgcaga aattaaagtt ccgcgaaccc cagcgcgagg aacgtatttg cttggttacc
      421 acaaattttc agacaaaatc aatgagctca atggttagcg atacgagctg tacttttccg
      481 agcagtgacg gtatcttttg gaaacactgg atccagacga aagacggcca atgcggatca
      541 ccgctggtga gtacccgtga cggctttatc gtgggcattc attccgccag caactttact
      601 aataccaata actactttac ctcagtaccc aagaatttta tggagctgtt aaccaaccaa
      661 gaagcacaac aatgggtgag cgggtggcgt ctgaacgccg atagcgttct gtggggtggt
      721 cacaaggtgt ttatggtcaa gcctgaggaa ccgttccaac ctgtgaaaga ggccacccag
      781 ttgatgaacg gttcgagaga cctaggctag gtggaggctc agtgatgata agtctgcgat
      841 ggtggatgca tgtgtcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcagag
      901 ggcacaatcc tattccgcgc tatccgacaa tctccaagac attaggtgga gttcagttcg
      961 gcgtatggca tatgtcgctg gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1021 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1081 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1141 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1201 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1261 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1321 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1381 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1441 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1501 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1561 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1621 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctctattcaa
     1681 caaagccgcc gtcccgtcaa gtcagcgtaa atgggtaggg ggcttcaaat cgtcccccca
     1741 tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     1801 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     1861 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     1921 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     1981 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     2041 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     2101 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     2161 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     2221 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     2281 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     2341 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     2401 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     2461 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     2521 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     2581 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     2641 cgatcaacgt ctcattgata cctgaaacaa aacccatcgt acggccaagg aagtctccaa
     2701 taactgtgat ccaccacaag cgccagggtt ttcccagtca cgacgtt
//
