LOCUS       sequence-318741-        6592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of AuroraA kinase-dead biosensor under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard
            O, Le Bras S, Gagne JP, Poirier GG, Le Borgne R, Prigent C, Tramier 
            M
  TITLE     Aurora kinase A localises to mitochondria to control organelle 
            dynamics and energy production.
  JOURNAL   eLife. 2018 Aug 2;7. pii: 38111. doi: 10.7554/eLife.38111.
  PUBMED    30070631
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-08-2"; volume: "7. pii: 38111. doi"; pages: " 
            10.7554/eLife.38111"
COMMENT     Sequence Label: pCMV superYFP-AURKA K162M-mTurq2
FEATURES             Location/Qualifiers
     source          1..6592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        136..327
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     284..304
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(329..433)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      460..915
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(547..566)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     757..778
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    922..1043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(933..952)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     987..1006
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1177..1198)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(1333..1845,1846..1848,1849..1851))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     1526..1545
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1785..1806
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2170..2181)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(3118..3777)
                     /codon_start=1
                     /product="tetrameric yellow fluorescent protein from 
                     Branchiostoma lanceolatum, also known as blFP-Y3"
                     /label=LanYFP
                     /translation="MASLPATHELHIFGSFNGVDFDMVGRGTGNPNDGYEELNLKSTKG
                     DLQFSPWILVPQIGYGFHQYLPFPDGMSPFQAAMKDGSGYQVHRTMQFEDGASLTSNYR
                     YTYEGSHIKGEFQVKGTGFPADGPVMTNSLTAADWCVTKMLYPNDKTIISTFDWTYTTG
                     NGKRYQSTARTTYTFAKPMAANILKNQPMFVFRKTELKHSKTELNFKEWQKAFTDVM"
     promoter        complement(3855..4058)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3884..3904)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4059..4362
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4537..5125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4617..4636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5454..5501
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5526..5545
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5733..6527)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5733..6527)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     6454..6473
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(6562..6592,1..327))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(6576..6592,1..119)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
       61 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
      121 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
      181 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
      241 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
      301 ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt
      361 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      421 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
      481 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      541 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      601 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      661 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      721 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      781 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      841 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
      901 taacgcttac aatttacgcc ttaagataca ttgatgagtt tggacaaacc acaactagaa
      961 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1021 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1081 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     1141 attatgatct agagtcgcgg ccgcttaggc ggcggtcacg aactccagca ggaccatgtg
     1201 atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt agtggttgtc
     1261 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     1321 cacgccgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
     1381 cttgtcggcg gtgatataga cgttgtcgct aaagtagttg tactccagct tgtgccccag
     1441 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     1501 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     1561 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1621 gtcggggtag cgggcgaagc actgcacgcc ccaggacagg gtggtcacga gggtgggcca
     1681 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     1741 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     1801 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca ttgcggccgc
     1861 catcggatcc ccagactgtt tgctagctga ttctttgttt tggcaatttg atggttttga
     1921 tgaatttgct gtgatccagg ggtgttcaag tacttctctg agcattggcc tctggctggg
     1981 attatgcttc aacagtcttg aaatgaggtc cctggctccc tctgttacaa agtcagggaa
     2041 tgtgaactca acccgtgata ttcttttgta ggtctcttgg tatgtgtttg cctcaaaagg
     2101 aggcttccca actaaaaatt catagcaaag aactccaagg ctccagagat ccaccttctc
     2161 atcatgcatc cgaccttcaa tcatttcagg gggcaggtag tccagggtgc cacagagagt
     2221 ggtcctcctg gaagatggag catgtactga ccacccaaaa tctgcaattt taagctctcc
     2281 agctgatcca agaagtaagt tctctggctt aatgtctcta tgaataactc tcttcgaatg
     2341 gcaatatgat agtgcatttg ccaattctgt tatataagta gcagttctct gctcatcaaa
     2401 ctttgaaagt ttctgaagtt ctctataaac tgttccaagt ggtgcatatt ccagaattag
     2461 gtagactctg gtagcatcat ggaaataacc atacagtcta agaatattag gatgccgaag
     2521 gtgggactgt atttctactt ctcttctgag ctgatgctcc actccggctt tctccagctg
     2581 agctttaaat aacaccataa gagccagaat aaacttgctt tgcttttctc ttgccaaata
     2641 aacattacca aactttcctt tacccagagg gcgaccaatt tcaaagtctt ccaaagccca
     2701 ctgcctcttt tttgattctt catttttctg ttttgatgcc agttcctcct caggattatt
     2761 ttcaggtgcc gatggcaggg gctgcttgct cttttgggtg ttattcagtg gcctggagac
     2821 aggatgaggt acactggttg cctgcaattg cttctgcttc tgattctgaa ccggcttgtg
     2881 actggagaca agtttttgtg cttgcaaagg aatgcgctgg gaagaatttg aaggacacaa
     2941 gacccgctga gcctggccac tatttacagg taatggattc tgacaaggaa attgctgagt
     3001 cacgagaaca cgttttggac ctccaactgg agctgtagcc ttaacaggtc ctgaaatgca
     3061 gttttcttta gatcggtcca tgaattcgaa gcttcccttg tacagctcgt ccatgcccat
     3121 cacatcggta aatgcctttt gccactcctt gaagttgagt tcggtcttgg agtgcttgag
     3181 ttccgtctta cggaacacga acatcggctg gttcttcagg atgttggccg ccattggctt
     3241 ggcaaaggtg taggtggtcc gcgcagtgct ctggtagcgc ttgccatttc cagtggtgta
     3301 agtccagtca aaggtgctga tgatggtttt gtcgttgggg tacagcatct tggtcacgca
     3361 ccagtccgca gcggtcagcg agttggtcat cacaggaccg tcagcaggga aaccagtccc
     3421 cttcacctga aactctcctt tgatgtggct tccctcgtag gtgtagcggt agttggaagt
     3481 cagggaggca ccgtcttcaa actgcattgt gcgatggact tggtatccgg agccatcttt
     3541 catggcggcc tggaaaggcg acatcccgtc ggggaagggc aggtactgat ggaatccata
     3601 cccgatttga gggaccagaa tccaggggga gaactggagg tcacccttgg tggacttcag
     3661 gtttaactcc tcataaccat catttggatt gccggtgcca cgacccacca tgtcaaagtc
     3721 cacaccgttg aaggagccaa agatgtgtaa ctcatgtgtc gctgggagag aggccatgtt
     3781 atcctcctct cctttggaga ccatctcgag atctgagtcc ggtagcgcta gcggatctga
     3841 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     3901 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     3961 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     4021 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     4081 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     4141 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     4201 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     4261 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     4321 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     4381 ctatgaacta atgaccccgt aattgattac tattaataac taatgcatgg cggtaatacg
     4441 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4501 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4561 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4621 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4681 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4741 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4801 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4861 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4921 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4981 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5041 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5101 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5161 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5221 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5281 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     5341 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     5401 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     5461 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     5521 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     5581 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     5641 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     5701 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     5761 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     5821 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     5881 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     5941 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     6001 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     6061 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     6121 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     6181 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     6241 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     6301 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     6361 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     6421 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     6481 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     6541 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ct
//
