LOCUS       sequence-318742-        5139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.G34V
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     304..323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     557..574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(733..760)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(852..938)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     987..1009
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1001..1018
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1002..1018
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1034..1133
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1136..3481
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSVIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     protein_bind    complement(3483..3582)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3599..3618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3600..3618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3623..3639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3623..3639)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4070..4861
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4954..5139,1..403)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
       61 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      181 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      361 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      421 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      481 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      541 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      601 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      661 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
      721 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      781 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      841 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      901 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      961 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
     1021 taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
     1081 aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagttg gcaccatggc
     1141 tactcaagct gatttgatgg agttggacat ggccatggaa ccagacagaa aagcggctgt
     1201 tagtcactgg cagcaacagt cttacctgga ctctgtaatc cattctggtg ccactaccac
     1261 agctccttct ctgagtggta aaggcaatcc tgaggaagag gatgtggata cctcccaagt
     1321 cctgtatgag tgggaacagg gattttctca gtccttcact caagaacaag tagctgatat
     1381 tgatggacag tatgcaatga ctcgagctca gagggtacga gctgctatgt tccctgagac
     1441 attagatgag ggcatgcaga tcccatctac acagtttgat gctgctcatc ccactaatgt
     1501 ccagcgtttg gctgaaccat cacagatgct gaaacatgca gttgtaaact tgattaacta
     1561 tcaagatgat gcagaacttg ccacacgtgc aatccctgaa ctgacaaaac tgctaaatga
     1621 cgaggaccag gtggtggtta ataaggctgc agttatggtc catcagcttt ctaaaaagga
     1681 agcttccaga cacgctatca tgcgttctcc tcagatggtg tctgctattg tacgtaccat
     1741 gcagaataca aatgatgtag aaacagctcg ttgtaccgct gggaccttgc ataacctttc
     1801 ccatcatcgt gagggcttac tggccatctt taagtctgga ggcattcctg ccctggtgaa
     1861 aatgcttggt tcaccagtgg attctgtgtt gttttatgcc attacaactc tccacaacct
     1921 tttattacat caagaaggag ctaaaatggc agtgcgttta gctggtgggc tgcagaaaat
     1981 ggttgccttg ctcaacaaaa caaatgttaa attcttggct attacgacag actgccttca
     2041 aattttagct tatggcaacc aagaaagcaa gctcatcata ctggctagtg gtggacccca
     2101 agctttagta aatataatga ggacctatac ttacgaaaaa ctactgtgga ccacaagcag
     2161 agtgctgaag gtgctatctg tctgctctag taataagccg gctattgtag aagctggtgg
     2221 aatgcaagct ttaggacttc acctgacaga tccaagtcaa cgtcttgttc agaactgtct
     2281 ttggactctc aggaatcttt cagatgctgc aactaaacag gaagggatgg aaggtctcct
     2341 tgggactctt gttcagcttc tgggttcaga tgatataaat gtggtcacct gtgcagctgg
     2401 aattctttct aacctcactt gcaataatta taagaacaag atgatggtct gccaagtggg
     2461 tggtatagag gctcttgtgc gtactgtcct tcgggctggt gacagggaag acatcactga
     2521 gcctgccatc tgtgctcttc gtcatctgac cagccgacac caagaagcag agatggccca
     2581 gaatgcagtt cgccttcact atggactacc agttgtggtt aagctcttac acccaccatc
     2641 ccactggcct ctgataaagg ctactgttgg attgattcga aatcttgccc tttgtcccgc
     2701 aaatcatgca cctttgcgtg agcagggtgc cattccacga ctagttcagt tgcttgttcg
     2761 tgcacatcag gatacccagc gccgtacgtc catgggtggg acacagcagc aatttgtgga
     2821 gggggtccgc atggaagaaa tagttgaagg ttgtaccgga gcccttcaca tcctagctcg
     2881 ggatgttcac aaccgaattg ttatcagagg actaaatacc attccattgt ttgtgcagct
     2941 gctttattct cccattgaaa acatccaaag agtagctgca ggggtcctct gtgaacttgc
     3001 tcaggacaag gaagctgcag aagctattga agctgaggga gccacagctc ctctgacaga
     3061 gttacttcac tctaggaatg aaggtgtggc gacatatgca gctgctgttt tgttccgaat
     3121 gtctgaggac aagccacaag attacaagaa acggctttca gttgagctga ccagctctct
     3181 cttcagaaca gagccaatgg cttggaatga gactgctgat cttggacttg atattggtgc
     3241 ccagggagaa ccccttggat atcgccagga tgatcctagc tatcgttctt ttcactctgg
     3301 tggatatggc caggatgcct tgggtatgga ccccatgatg gaacatgaga tgggtggcca
     3361 ccaccctggt gctgactatc cagttgatgg gctgccagat ctggggcatg cccaggacct
     3421 catggatggg ctgcctccag gtgacagcaa tcagctggcc tggtttgata ctgacctgta
     3481 attgacaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     3541 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     3601 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     3661 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     3721 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     3781 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     3841 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     3901 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     3961 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     4021 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     4081 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     4141 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     4201 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     4261 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     4321 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     4381 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     4441 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     4501 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     4561 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     4621 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     4681 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     4741 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     4801 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     4861 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     4921 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4981 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5041 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5101 tcttctagtg tagccgtagt taggccacca cttcaagaa
//
