LOCUS       sequence-318759-       10803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation transfer vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Larkins CE, Aviles GD, East MP, Kahn RA, Caspary T
  TITLE     Arl13b regulates ciliogenesis and the dynamic localization of Shh 
            signaling proteins.
  JOURNAL   Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5.
  PUBMED    21976698
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2011-12"; volume: "22(23)"; pages: "4694-703. Epub 
            2011 Oct 5"
COMMENT     Sequence Label: L13-Arl13bGFP
FEATURES             Location/Qualifiers
     source          1..10803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2684..2987
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2988..3191
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    3224..3248
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             3260..3280
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(4587..4611)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4637..4646
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4643..4645,4646..4648,4649..5362)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4643..4645,4646..4648,4649..5359)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4688..4709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4949..4968)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5296..5317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5495..6083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5548..5568)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5966..5977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6086..6102)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6087..6103)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6608..6788
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6814..6831)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6820..7044
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7090..7518
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7177..7196)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7387..7408
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7527..7547)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7532..7861
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7712..7847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7774..7793
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7909..7956
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7975..8349
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8479..8600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8516..8535)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8570..8589
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8649..8665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8649..8665)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8662..8684)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8673..8689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8697..8703,8704..8721,8722..8727))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8742..8763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8880..8897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9051..9639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9131..9150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9810..10601,10602..10670))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10433..10452
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10671..10775)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg ataaccgtat taccgccatg cattagttat taatagtaat
     2641 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2701 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2761 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2821 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2881 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     2941 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3001 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3061 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3121 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3181 taagcagagc tggtttagtg aaccgtcaga tccgctagct caaacaagtt tgtacaaaaa
     3241 agcaggctcc gcggccgcct tgtttaactt taagaaggag cccttcacca tgttcagtct
     3301 gatggccaac tgctgcaact tgttcaagcg gtggcgggag cctgtcagaa aggtgacact
     3361 tgtgatggtc ggccttgata atgctggtaa aacagccaca gcaaagggaa tccaaggaga
     3421 gcatcctgaa gacgtagctc ccactgttgg cttttctaaa attgatctga gacaaggaaa
     3481 gttccaagtt accatctttg acttaggagg tggaaaaaga attcggggaa tttggaagaa
     3541 ttattatgct gaatcctatg gggtaatatt tgttgtggat tccagtgatg aggagagaat
     3601 ggaagaaaca aaggagacaa tgtccgaagt gctaagacac ccgaggatat caggaaagcc
     3661 tatattggtg ctggccaaca agcaagacaa ggagggggct ttaggcgaag cggatgtgat
     3721 tgagtgtctc tcgctggaga agctggtcaa cgagcacaag tgcctgtgtc agatcgaacc
     3781 ttgttcagca gtcttgggat atggaaagaa aattgacaag tccattaaaa aggggcttta
     3841 ttggctactg catatcattg caaaggactt tgatgcctta agtgaacgca tccagaaaga
     3901 cacaactgaa cagcgagctc ttgaggaaca agagaaacgt gagagggctg aacgagtccg
     3961 gaagttaagg gaagaaagag aacgagagca gacggaactt gatgggacca gtggtctggc
     4021 tgagattgac tcaggaccag ttcttgcgaa tcctttccaa cccattgcag ctgtaatcat
     4081 tgagaatgaa aagaagcagg aaaaagagaa aaagaagcag accgtggaga aggacagtga
     4141 tgttggtctc ctagaacata aagtggagcc agagcaagca gcgcctcaga gtgaggcaga
     4201 ttgctgcctc caaaaccctg acgagcgagt ggtggacagt tacagggagg cactgtcgca
     4261 gcagctggac agtgaggatg agcaggacca gcgagggtca gagtccgggg aaaacagtaa
     4321 gaagaaaacc aagaaactaa gaatgaaaag gagtcatcgg gtagaaccag tcaatacaga
     4381 cgagtctact ccaaagagtc ccacgcctcc ccaacctccc cctcctgttg gctggggaac
     4441 ccccaaagtc actagacttc caaaacttga gcctcttggt gaaacacgtc acaatgattt
     4501 ctatgggaag ccgctgcctc ccctggctgt gcgacagaga cctaacggtg atgctcagga
     4561 cacgatctca aagggtgggc gcgccgaccc agctttcttg tacaaagtgg ttcgatctag
     4621 aatggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     4681 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4741 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     4801 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     4861 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4921 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     4981 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5041 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5101 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     5161 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5221 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     5281 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5341 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     5401 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5461 aaaatgaatg caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     5521 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5581 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5641 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5701 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5761 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5821 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5881 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     5941 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6001 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6061 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     6121 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     6181 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     6241 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6301 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     6361 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     6421 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     6481 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     6541 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     6601 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6661 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6721 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6781 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     6841 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6901 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6961 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7021 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     7081 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7141 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7201 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7261 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7321 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7381 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7441 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7501 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7561 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7621 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7681 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7741 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7801 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7861 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     7921 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     7981 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     8041 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     8101 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     8161 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     8221 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     8281 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     8341 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8401 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8461 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8521 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8581 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     8641 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8701 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8761 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8821 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8881 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8941 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9001 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9061 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9121 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9181 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9241 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9301 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9361 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9421 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9481 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9541 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9601 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9661 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9721 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9781 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9841 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9901 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9961 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10021 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10081 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10141 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10201 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10261 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10321 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10381 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10441 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10501 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10561 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10621 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10681 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10741 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10801 gac
//
