LOCUS       sequence-318762-       13503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector containing PIK3C2A tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13503)
  AUTHORS   Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, 
            McGarry L, Shanks E, Mostov KE, Bryant DM
  TITLE     The phospholipid PI(3,4)P2 is an apical identity determinant.
  JOURNAL   Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
  PUBMED    30487552
REFERENCE   2  (bases 1 to 13503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-28"; volume: "9(1):5041. doi"; pages: " 
            10.1038/s41467-018-07464-8"
COMMENT     Sequence Label: pLEX303 GFP-PIK3C2A
FEATURES             Location/Qualifiers
     source          1..13503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      612..621
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(618..620,621..623,624..1334)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(618..620,621..623,624..1334)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(663..684)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(924..943)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1271..1292
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6453..6470
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6513..7101
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6566..6586)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6984..6995)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7173..7406
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7484..7605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7521..7540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7575..7594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7645..7780
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7800..7819)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7801..7819)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7829..7846)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7829..7845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7838..7860)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7987..8442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8074..8093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8284..8305
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8468..8572
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8573..8641,8642..9433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8791..8810)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9604..10192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10093..10112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10346..10363
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10480..10501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10516..10521,10522..10539,10540..10546)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10554..10570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10559..10581
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10578..10594
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10578..10594
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10613..10633
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10615..10633
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10661..10887
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10888..11068
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11115..11240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11733..11966
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12151..12195
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12370..12870
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     12789..12807
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             12882..13277
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    13337..13454
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gggcccgggg ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agccctgcag ggccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      661 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      721 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      781 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      841 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      901 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      961 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1021 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     1081 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1141 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1201 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     1261 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1321 acgagctgta caagtactca gatctcgagc tccaccgcgg tggcggccgc tctagaacta
     1381 gtatggctca gatatttagc aacagcggat ttaaagaatg tccatcttca catccggaac
     1441 caacaagagc aaaagatgtg gacaaagaag aagcattaca gatggaagca gaggctttag
     1501 caaaactgca aaaggataga caagtgactg acaatcagag aggctttgag ttgtcaagca
     1561 gcaccagaaa aaaagcacag gtttataaca agcaggatta tgatctcatg gtgtttcctg
     1621 aatcagattc ccaaaaaaga gcattagata ttgatgtaga aaagctcacc caagctgaac
     1681 ttgagaaact attgctggat gacagtttcg agactaaaaa aacacctgta ttaccagtta
     1741 ctcctattct gagcccttcc ttttcagcac agctctattt tagacctact attcagagag
     1801 gacagtggcc acctggatta cctgggcctt ccacttatgc tttaccttct atttatcctt
     1861 ctacttacag taaacaggct gcattccaaa atggcttcaa tccaagaatg cccacttttc
     1921 catctacaga acctatatat ttaagtcttc cgggacaatc tccatatttc tcatatcctt
     1981 tgacacctgc cacacccttt catccacaag gaagcttacc tatctatcgt ccagtagtca
     2041 gtactgacat ggcaaaacta tttgacaaaa tagctagtac atcagaattt ttaaaaaatg
     2101 ggaaagcaag gactgatttg gagataacag attcaaaagt cagcaatcta caggtatctc
     2161 caaagtctga ggatatcagt aaatttgact ggttagactt ggatcctcta agtaagccta
     2221 aggtggataa tgtggaggta ttagaccatg aggaagagaa aaatgtttca agtttgctag
     2281 caaaggatcc ttgggatgct gttcttcttg aagagagatc gacagcaaat tgtcatcttg
     2341 aaagaaaggt gaatggaaaa tccctttctg tggcaactgt tacaagaagc cagtctttaa
     2401 atattcgaac aactcagctt gcaaaagccc agggccatat atctcagaaa gacccaaatg
     2461 ggaccagtag tttgccaact ggaagttctc ttcttcaaga agttgaagta cagaatgagg
     2521 agatggcagc tttttgtcga tccattacaa aattgaagac caaatttcca tataccaatc
     2581 accgcacaaa cccaggctat ttgttaagtc cagtcacagc gcaaagaaac atatgcggag
     2641 aaaatgctag tgtgaaggtc tccattgaca ttgaaggatt tcagctacca gttactttta
     2701 cgtgtgatgt gagttctact gtagaaatca ttataatgca agccctttgc tgggtacatg
     2761 atgacttgaa tcaagtagat gttggcagct atgttctaaa agtttgtggt caagaggaag
     2821 tgctgcagaa taatcattgc cttggaagtc atgagcatat tcaaaactgt cgaaaatggg
     2881 acacagaaat tagactacaa ctcttgacct tcagtgcaat gtgtcaaaat ctggcccgaa
     2941 cagcagaaga tgatgaaaca cccgtggatt taaacaaaca cctgtatcaa atagaaaaac
     3001 cttgcaaaga agccatgacg agacaccctg ttgaagaact cttagattct tatcacaacc
     3061 aagtagaact ggctcttcaa attgaaaacc aacaccgagc agtagatcaa gtaattaaag
     3121 ctgtaagaaa aatctgtagt gctttagatg gtgtcgagac tcttgccatt acagaatcag
     3181 taaagaagct aaagagagca gttaatcttc caaggagtaa aactgctgat gtgacttctt
     3241 tgtttggagg agaagacact agcaggagtt caactagggg ctcacttaat cctgaaaatc
     3301 ctgttcaagt aagcataaac caattaactg cagcaattta tgatcttctc agactccatg
     3361 caaattctgg taggagtcct acagactgtg cccaaagtag caagagtgtc aaggaagcat
     3421 ggactacaac agagcagctc cagtttacta tttttgctgc tcatggaatt tcaagtaatt
     3481 gggtatcaaa ttatgaaaaa tactacttga tatgttcact gtctcacaat ggaaaggatc
     3541 tttttaaacc tattcaatca aagaaggttg gcacttacaa gaatttcttc tatcttatta
     3601 aatgggatga actaatcatt tttcctatcc agatatcaca attgccatta gaatcagttc
     3661 ttcaccttac tctttttgga attttaaatc agagcagtgg aagttcccct gattctaata
     3721 agcagagaaa gggaccagaa gctttgggca aagtttcttt acctcttttt gactttaaac
     3781 ggtttttaac atgtggaact aaacttctat atctttggac ttcatcacat acaaattctg
     3841 ttcctggaac agttaccaaa aaaggatatg tcatggaaag aatagtgcta caggttgatt
     3901 ttccttctcc tgcatttgat attatttata caactcctca agttgacaga agcattatac
     3961 agcaacataa cttagaaaca ctagagaatg atataaaagg gaaacttctt gatattcttc
     4021 ataaagactc atcacttgga ctttctaaag aagataaagc ttttttatgg gagaaacgtt
     4081 attattgctt caaacaccca aattgtcttc ctaaaatatt agcaagcgcc ccaaactgga
     4141 aatgggttaa tcttgccaaa acttactcat tgcttcacca gtggcctgca ttgtacccac
     4201 taattgcatt ggaacttctt gattcaaaat ttgctgatca ggaagtaaga tccctagctg
     4261 tgacctggat tgaggccatt agtgatgatg agctaacaga tcttcttcca cagtttgtac
     4321 aagctttgaa atatgaaatt tacttgaata gttcattagt gcaattcctt ttgtccaggg
     4381 cattgggaaa tatccagata gcacacaatt tatattggct tctcaaagat gccctgcatg
     4441 atgtacagtt tagtacccga tacgaacatg ttttgggtgc tctcctgtca gtaggaggaa
     4501 aacgacttag agaagaactt ctaaaacaga cgaaacttgt acagctttta ggaggagtag
     4561 cagaaaaagt aaggcaggct agtggatcag ccagacaggt tgttctccaa agaagtatgg
     4621 aacgagtaca gtcctttttt cagaaaaata aatgccgtct ccctctcaag ccaagtctag
     4681 tggcaaaaga attaaatatt aagtcgtgtt ccttcttcag ttctaatgct gtccccctaa
     4741 aagtcacaat ggtgaatgct gaccctatgg gagaagaaat taatgtcatg tttaaggttg
     4801 gtgaagatct tcggcaagat atgttagctt tacagatgat aaagattatg gataagatct
     4861 ggcttaaaga aggactagat ctgaggatgg taattttcaa atgtctctca actggcagag
     4921 atcgaggcat ggtggagctg gttcctgctt ccgataccct caggaaaatc caagtggaat
     4981 atggtgtgac aggatccttt aaagataaac cacttgcaga gtggctaagg aaatacaatc
     5041 cctctgaaga agaatatgaa aaggcttcag agaactttat ctattcctgt gctggatgct
     5101 gtgtagccac ctatgtttta ggcatctgtg atcgacacaa tgacaatata atgcttcgaa
     5161 gcacgggaca catgtttcac attgactttg gaaagttttt gggacatgca cagatgtttg
     5221 gcagcttcaa aagggatcgg gctccttttg tgctgacctc tgatatggca tatgtcatta
     5281 atgggggtga aaagcccacc attcgttttc agttgtttgt ggacctctgc tgtcaggcct
     5341 acaacttgat aagaaagcag acaaaccttt ttcttaacct cctttcactg atgattcctt
     5401 cagggttacc agaacttaca agtattcaag atttgaaata cgttagagat gcacttcaac
     5461 cccaaactac agacgcagaa gctacaattt tctttactag gcttattgaa tcaagtttgg
     5521 gaagcattgc cacaaagttt aacttcttca ttcacaacct tgctcagctt cgtttttctg
     5581 gtcttccttc taatgatgag cccatccttt cattttcacc taaaacatac tcctttagac
     5641 aagatggtcg aatcaaggaa gtctctgttt ttacatatca taagaaatac aacccagata
     5701 aacattatat ttatgtagtc cgaattttga gggaaggaca gattgaacca tcatttgtct
     5761 tccgaacatt tgacgaattt caggaacttc acaataagct cagtattatt tttccacttt
     5821 ggaagttacc aggctttcct aataggatgg ttctaggaag aacacacata aaagatgtag
     5881 cagccaaaag gaaaattgag ttaaacagtt acttacagag tttgatgaat gcttcaacgg
     5941 atgtagcaga gtgtgatctt gtttgtactt tcttccaccc tttacttcgt gatgagaaag
     6001 ctgaagggat agctaggtct gcagatgcag gttccttcag tcctactcca ggccaaatag
     6061 gaggagctgt gaaattatcc atctcttacc gaaatggtac tcttttcatc atggtgatgc
     6121 atatcaaaga tcttgttact gaagatggag ctgacccaaa tccatatgtc aaaacatacc
     6181 tacttccaga taaccacaaa acatccaaac gtaaaaccaa aatttcacga aaaacgagga
     6241 atccgacatt caatgaaatg cttgtataca gtggatatag caaagaaacc ctaagacagc
     6301 gagaacttca actaagtgta ctcagtgcag aatctctgcg ggagaatttt ttcttgggtg
     6361 gagtaaccct gcctttgaaa gatttcaact tgagcaaaga gacggttaaa tggtatcagc
     6421 tgactgcggc aacatacttg taattaatta accaccacca ccaccaccac taaggatccc
     6481 gtacgtgatc accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     6541 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6601 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6661 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6721 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6781 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6841 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6901 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6961 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7021 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7081 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     7141 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7201 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7261 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7321 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7381 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     7441 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7501 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7561 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7621 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7681 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7741 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     7801 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     7861 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     7921 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7981 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8041 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8101 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8161 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8221 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8281 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8341 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8401 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     8461 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8521 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8581 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8641 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8701 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8761 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8821 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8881 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8941 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9001 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9061 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9121 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9181 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9241 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9301 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9361 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9421 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9481 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9541 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9721 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10201 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10261 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10321 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    10381 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    10441 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    10501 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    10561 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
    10621 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
    10681 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
    10741 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
    10801 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
    10861 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
    10921 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10981 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    11041 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
    11101 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
    11161 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
    11221 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
    11281 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
    11341 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
    11401 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
    11461 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
    11521 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
    11581 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
    11641 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
    11701 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
    11761 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
    11821 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
    11881 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
    11941 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    12001 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    12061 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    12121 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    12181 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    12241 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    12301 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    12361 aggggaccct tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
    12421 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
    12481 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
    12541 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
    12601 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
    12661 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
    12721 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
    12781 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
    12841 gttgaccgaa tcaccgacct ctctccccag ggggtaccac catggccaag cctttgtctc
    12901 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
    12961 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
    13021 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
    13081 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
    13141 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
    13201 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
    13261 gttatgtgtg ggagggcctg cagctgcagt agtaagaatt ctagatcttg agacaaatgg
    13321 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
    13381 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
    13441 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
    13501 agg
//
