LOCUS       sequence-318763-       10654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q 
            mutant) driven by human EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Au YZ, Gu M, De Braekeleer E, Gozdecka M, Aspris D, Tarumoto Y, 
            Cooper J, Yu J, Ong SH, Chen X, Tzelepis K, Huntly BJP, Vassiliou G,
            Yusa K
  TITLE     KAT7 is a genetic vulnerability of acute myeloid leukemias driven by
            MLL rearrangements.
  JOURNAL   Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. 
            Epub 2020 Aug 6.
  PUBMED    32764680
REFERENCE   2  (bases 1 to 10654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-020-1001-z"; journalName: "Leukemia"; date: 
            "2021-04"; volume: "35"; issue: "4"; pages: "1012-1022"
COMMENT     Sequence Label: pKLV-EF1aGFP2AKAT7(E508Q)-W
FEATURES             Location/Qualifiers
     source          1..10654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             218..398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    445..570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1067..1300
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1485..1529
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1827..1944
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2415..3596
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2646..3588
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3545..3565
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3609..3618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3615..3617,3618..3620,3621..4331)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3615..3617,3618..3620,3621..4331)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3660..3681)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3921..3940)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4268..4289
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4350..4403
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6311..6331)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6729..6740)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7192..7209)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7198..7422
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7448..7468)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7448..7466)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7487..7503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7487..7503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7500..7522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7511..7527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7535..7541,7542..7559,7560..7565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7580..7601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7718..7735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7889..8477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7969..7988)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8648..9439,9440..9508))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9271..9290
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9509..9613)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9639..10094)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9776..9797)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9988..10007
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10221..10243
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10235..10252
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10236..10252
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10262..10281
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10262..10280
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        10351..10654
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      901 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
      961 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1021 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1081 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1141 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1201 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1261 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1321 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1381 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1441 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1501 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1561 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1621 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1681 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1741 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     1801 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     1861 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     1921 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     1981 ttagtaccgg gccctacgcg tgaggggcgc ggaagccggg gcggaggagg agccaacccc
     2041 gggctgggct gagacccgca gaggaagacg ctctagggat ttgtcccgga ctagcgagat
     2101 ggcaaggctg aggacgggag gctgattgag aggcgaaggt acaccctaat ctcaatacaa
     2161 cctttggagc taagccagca atggtagagg gaagattctg cacgtccctt ccaggcggcc
     2221 tccccgtcac cacccccccc aacccgcccc gaccggagct gagagtaatt catacaaaag
     2281 gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact aacgtagaac
     2341 ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac tgaggtggag
     2401 aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     2461 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaaag tggcgcgggg
     2521 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     2581 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     2641 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     2701 gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga tcccgagctt
     2761 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2821 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2881 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2941 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     3001 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     3061 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     3121 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     3181 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     3241 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3301 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3361 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3421 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3481 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3541 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtggaag
     3601 cttggcgcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3661 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3721 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     3781 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     3841 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3901 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     3961 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4021 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     4081 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     4141 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4201 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     4261 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4321 agctgtacaa gggcggcggg tccggaggag agggcagagg aagtctccta acatgcggtg
     4381 acgtggagga gaatcctggc ccaatgccgc gaaggaagag gaatgcaggc agtagttcag
     4441 atggaaccga agattccgat ttttctacag atctcgagca cacagacagt tcagaaagtg
     4501 atggcacatc ccgacgatct gctcgagtca cccgctcctc agccaggcta agccagagtt
     4561 ctcaagattc cagtcctgtt cgaaatctgc agtcttttgg cactgaggag cctgcttact
     4621 ctaccagaag agtgacccgt agtcagcagc agcctacccc agtgacaccg aaaaaatacc
     4681 ctcttcggca gactcgttca tctggttcag aaactgagca agtggttgat ttttcagata
     4741 gagaaactaa aaatacagct gatcatgatg agtcaccgcc tcgaactcca actggaaatg
     4801 cgccttcttc tgagtctgac atagacatct ccagccccaa tgtatctcac gatgagagca
     4861 ttgccaagga catgtccctg aaggactcag gcagtgatct ctctcatcgc cccaagcgcc
     4921 gtcgcttcca tgaaagctac aacttcaata tgaagtgtcc tacaccaggc tgtaactctc
     4981 taggacacct tacaggaaaa catgagagac atttctccat ctcaggatgc ccactgtatc
     5041 ataacctctc agctgacgaa tgcaaggtga gagcacagag ccgggataag cagatagaag
     5101 aaaggatgct gtctcacagg caagatgaca acaacaggca tgcaaccagg caccaggcac
     5161 caacggagag acagcttcga tataaggaaa aagtggctga actcaggaag aaaagaaatt
     5221 ctggactgag caaagaacag aaagagaaat atatggaaca cagacagacc tatgggaaca
     5281 cacgggaacc tcttttagaa aacctgacaa gcgagtatga cttggatctt ttccgaagag
     5341 cacaagcccg ggcttcagag gatttggaga agttaaggct gcaaggccaa atcacagagg
     5401 gaagcaacat gattaaaaca attgcttttg gccgctacga gctggacaca tggtatcatt
     5461 ctccatatcc tgaagaatat gcacggctgg gacgtctcta tatgtgtgaa ttctgtttaa
     5521 aatatatgaa gagccaaacg atactccgcc ggcacatggc caaatgtgtg tggaaacacc
     5581 cacctggtga tgagatatat cgcaaaggtt caatctctgt gtttgaagtg gatggcaaga
     5641 aaaacaagat ctactgccaa aacctgtgcc tgttggccaa actttttctg gaccacaaga
     5701 cattatatta tgatgtggag cccttcctgt tctatgttat gacagaggcg gacaacactg
     5761 gctgtcacct gattggatat ttttctaagg aaaagaattc attcctcaac tacaacgtct
     5821 cctgtatcct tactatgcct cagtacatga gacagggcta tggcaagatg cttattgatt
     5881 tcagttattt gctttccaaa gtcgaagaaa aagttggctc cccacagcgt ccactctcag
     5941 atctggggct tataagctat cgcagttact ggaaagaagt acttctccgc tatctccaca
     6001 acttccaagg caaagagatt tctatcaaag aaatcagtca ggagacggct gtgaatcctg
     6061 tggacattgt cagcactctg caagcccttc agatgctcaa atactggaag ggaaaacacc
     6121 tagttttaaa gagacaggac ctgattgatg agtggatagc caaagaggcc aaaaggtcca
     6181 actccaataa aaccatggat cccagctgct taaaatggac ccctcccaag ggcacttaaa
     6241 agtaagaatt cgcggccgct caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6301 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6361 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6421 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6481 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6541 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6601 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     6661 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6721 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6781 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6841 ccccgcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6901 taaaagaaaa ggggggactg gaagggctaa ttcactccca aagaagtcaa gatctgcttt
     6961 ttgcctgtac tgggtctctc tggttagacc agagtctctc tggttagacc agatctgagc
     7021 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7081 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7141 acccttttag tcagtgtgga aaatctctag cagtttaaac ccgctgatca gcctcgactg
     7201 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     7261 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     7321 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     7381 aagtcaatag caggcatgct ggggatgcgg tgggctctat gggcggccgt taattaagct
     7441 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     7501 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccggg agcataaagt
     7561 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7621 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7681 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7741 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7801 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7861 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7921 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8941 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9601 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     9661 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     9721 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     9781 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     9841 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     9901 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     9961 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
    10021 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
    10081 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10141 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10201 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
    10261 gtaatacgac tcactatagg gcgaattgac tagttattaa tagtaatcaa ttacggggtc
    10321 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
    10381 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
    10441 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
    10501 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
    10561 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
    10621 gtacatctac gtattagtca tcgctattac catg
//
